Nitric oxide enhances paracellular permeability of opossum kidney cells
Nitric oxide enhances paracellular permeability of opossum kidney cells. Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tub...
Gespeichert in:
| Veröffentlicht in: | Kidney international 1999-06, Vol.55 (6), p.2215-2223 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2223 |
|---|---|
| container_issue | 6 |
| container_start_page | 2215 |
| container_title | Kidney international |
| container_volume | 55 |
| creator | Liang, Mingyu Knox, Franklyn G. |
| description | Nitric oxide enhances paracellular permeability of opossum kidney cells.
Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tubule cell line that possesses a leaky paracellular pathway resembling that of the in vivo proximal tubule.
Paracellular permeability of OK cells cultured on permeable supports was measured as the apparent paracellular permeability coefficient (Papp) for 3[H]-D-mannitol. Changes in cell viability, cellular adenosine triphosphate (ATP) content, cGMP levels, and lipid peroxidation were assessed.
Incubation with 2mm sodium nitroprusside (SNP), an NO donor, for 24hours significantly enhanced the Papp of OK cell sheets by 30.6 ± 7.0% (N = 8, P < 0.05). This effect was largely blunted by hemoglobin, a NO scavenger. Cell viability was not compromised. This effect of SNP was concomitant with a moderate reduction of cellular ATP content, an increase in lipid peroxidation, and an increase in cellular cGMP levels. The antioxidant superoxide dismutase (SOD) significantly attenuated the effect of SNP on cellular ATP content and blunted the increase in Papp caused by SNP. A soluble guanylate cyclase inhibitor did not affect the effect of SNP on the Papp.
NO enhances the paracellular permeability of OK cells possibly via mechanisms involving decreases in cellular ATP content. |
| doi_str_mv | 10.1046/j.1523-1755.1999.00465.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69799597</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0085253815461848</els_id><sourcerecordid>69799597</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-91583dc936c9405e50ea639b20f1b0839ec641fff216bed8d17f5d6ec22e3673</originalsourceid><addsrcrecordid>eNqFkMFOxCAQhonR6Lr6CoaD8dYKpbTlqEZXE6OXvRMKQ2RttxVas_v2Unej3jxNZvhm-PMhhClJKcmL61VKecYSWnKeUiFESuKUp5sDNPt5OEQzQiqeZJxVJ-g0hBWJvWDkGJ1QwnielWSGFi9u8E7jbuMMYFi_qbWGgHvllYamGRvlcQ--BVW7xg1b3Fnc9V0IY4vfnVnDFk9cOENHVjUBzvd1jpYP98u7x-T5dfF0d_Oc6DwXQyIor5jRghVa5IQDJ6AKJuqMWFqTignQRU6ttRktajCVoaXlpgCdZcCKks3R1e5s77uPEcIgWxemAGoN3RhkIUohuJjAagdqH8N6sLL3rlV-KymRk0O5kpMqOamSk0P57VBu4urF_o-xbsH8WdxJi8DlHlBBq8b66MyFX64iPMtFxG53GEQfnw68DNpB1GucBz1I07n_w3wBiBCQRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69799597</pqid></control><display><type>article</type><title>Nitric oxide enhances paracellular permeability of opossum kidney cells</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Liang, Mingyu ; Knox, Franklyn G.</creator><creatorcontrib>Liang, Mingyu ; Knox, Franklyn G.</creatorcontrib><description>Nitric oxide enhances paracellular permeability of opossum kidney cells.
Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tubule cell line that possesses a leaky paracellular pathway resembling that of the in vivo proximal tubule.
Paracellular permeability of OK cells cultured on permeable supports was measured as the apparent paracellular permeability coefficient (Papp) for 3[H]-D-mannitol. Changes in cell viability, cellular adenosine triphosphate (ATP) content, cGMP levels, and lipid peroxidation were assessed.
Incubation with 2mm sodium nitroprusside (SNP), an NO donor, for 24hours significantly enhanced the Papp of OK cell sheets by 30.6 ± 7.0% (N = 8, P < 0.05). This effect was largely blunted by hemoglobin, a NO scavenger. Cell viability was not compromised. This effect of SNP was concomitant with a moderate reduction of cellular ATP content, an increase in lipid peroxidation, and an increase in cellular cGMP levels. The antioxidant superoxide dismutase (SOD) significantly attenuated the effect of SNP on cellular ATP content and blunted the increase in Papp caused by SNP. A soluble guanylate cyclase inhibitor did not affect the effect of SNP on the Papp.
NO enhances the paracellular permeability of OK cells possibly via mechanisms involving decreases in cellular ATP content.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1046/j.1523-1755.1999.00465.x</identifier><identifier>PMID: 10354270</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; antioxidants ; Antioxidants - pharmacology ; Biological and medical sciences ; Cell Membrane Permeability - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Cyclic GMP - metabolism ; cytotoxicity ; Investigative techniques, diagnostic techniques (general aspects) ; Kidney Tubules, Proximal - cytology ; Kidney Tubules, Proximal - drug effects ; Kidney Tubules, Proximal - metabolism ; L-Lactate Dehydrogenase - metabolism ; Lipid Peroxidation - drug effects ; Mannitol - metabolism ; Medical sciences ; Nitric Oxide - metabolism ; Nitric Oxide Donors - pharmacology ; Nitroprusside - pharmacology ; Opossums ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; proximal tubule ; Superoxide Dismutase - pharmacology ; tight junctions ; transport ; Urinary system</subject><ispartof>Kidney international, 1999-06, Vol.55 (6), p.2215-2223</ispartof><rights>1999 International Society of Nephrology</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-91583dc936c9405e50ea639b20f1b0839ec641fff216bed8d17f5d6ec22e3673</citedby><cites>FETCH-LOGICAL-c449t-91583dc936c9405e50ea639b20f1b0839ec641fff216bed8d17f5d6ec22e3673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23910,23911,25119,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1805249$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10354270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Mingyu</creatorcontrib><creatorcontrib>Knox, Franklyn G.</creatorcontrib><title>Nitric oxide enhances paracellular permeability of opossum kidney cells</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>Nitric oxide enhances paracellular permeability of opossum kidney cells.
Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tubule cell line that possesses a leaky paracellular pathway resembling that of the in vivo proximal tubule.
Paracellular permeability of OK cells cultured on permeable supports was measured as the apparent paracellular permeability coefficient (Papp) for 3[H]-D-mannitol. Changes in cell viability, cellular adenosine triphosphate (ATP) content, cGMP levels, and lipid peroxidation were assessed.
Incubation with 2mm sodium nitroprusside (SNP), an NO donor, for 24hours significantly enhanced the Papp of OK cell sheets by 30.6 ± 7.0% (N = 8, P < 0.05). This effect was largely blunted by hemoglobin, a NO scavenger. Cell viability was not compromised. This effect of SNP was concomitant with a moderate reduction of cellular ATP content, an increase in lipid peroxidation, and an increase in cellular cGMP levels. The antioxidant superoxide dismutase (SOD) significantly attenuated the effect of SNP on cellular ATP content and blunted the increase in Papp caused by SNP. A soluble guanylate cyclase inhibitor did not affect the effect of SNP on the Papp.
NO enhances the paracellular permeability of OK cells possibly via mechanisms involving decreases in cellular ATP content.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cyclic GMP - metabolism</subject><subject>cytotoxicity</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kidney Tubules, Proximal - cytology</subject><subject>Kidney Tubules, Proximal - drug effects</subject><subject>Kidney Tubules, Proximal - metabolism</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Mannitol - metabolism</subject><subject>Medical sciences</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Nitroprusside - pharmacology</subject><subject>Opossums</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>proximal tubule</subject><subject>Superoxide Dismutase - pharmacology</subject><subject>tight junctions</subject><subject>transport</subject><subject>Urinary system</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFOxCAQhonR6Lr6CoaD8dYKpbTlqEZXE6OXvRMKQ2RttxVas_v2Unej3jxNZvhm-PMhhClJKcmL61VKecYSWnKeUiFESuKUp5sDNPt5OEQzQiqeZJxVJ-g0hBWJvWDkGJ1QwnielWSGFi9u8E7jbuMMYFi_qbWGgHvllYamGRvlcQ--BVW7xg1b3Fnc9V0IY4vfnVnDFk9cOENHVjUBzvd1jpYP98u7x-T5dfF0d_Oc6DwXQyIor5jRghVa5IQDJ6AKJuqMWFqTignQRU6ttRktajCVoaXlpgCdZcCKks3R1e5s77uPEcIgWxemAGoN3RhkIUohuJjAagdqH8N6sLL3rlV-KymRk0O5kpMqOamSk0P57VBu4urF_o-xbsH8WdxJi8DlHlBBq8b66MyFX64iPMtFxG53GEQfnw68DNpB1GucBz1I07n_w3wBiBCQRg</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Liang, Mingyu</creator><creator>Knox, Franklyn G.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Nitric oxide enhances paracellular permeability of opossum kidney cells</title><author>Liang, Mingyu ; Knox, Franklyn G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-91583dc936c9405e50ea639b20f1b0839ec641fff216bed8d17f5d6ec22e3673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cyclic GMP - metabolism</topic><topic>cytotoxicity</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kidney Tubules, Proximal - cytology</topic><topic>Kidney Tubules, Proximal - drug effects</topic><topic>Kidney Tubules, Proximal - metabolism</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Mannitol - metabolism</topic><topic>Medical sciences</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Nitroprusside - pharmacology</topic><topic>Opossums</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>proximal tubule</topic><topic>Superoxide Dismutase - pharmacology</topic><topic>tight junctions</topic><topic>transport</topic><topic>Urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Mingyu</creatorcontrib><creatorcontrib>Knox, Franklyn G.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Mingyu</au><au>Knox, Franklyn G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide enhances paracellular permeability of opossum kidney cells</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>55</volume><issue>6</issue><spage>2215</spage><epage>2223</epage><pages>2215-2223</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract>Nitric oxide enhances paracellular permeability of opossum kidney cells.
Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tubule cell line that possesses a leaky paracellular pathway resembling that of the in vivo proximal tubule.
Paracellular permeability of OK cells cultured on permeable supports was measured as the apparent paracellular permeability coefficient (Papp) for 3[H]-D-mannitol. Changes in cell viability, cellular adenosine triphosphate (ATP) content, cGMP levels, and lipid peroxidation were assessed.
Incubation with 2mm sodium nitroprusside (SNP), an NO donor, for 24hours significantly enhanced the Papp of OK cell sheets by 30.6 ± 7.0% (N = 8, P < 0.05). This effect was largely blunted by hemoglobin, a NO scavenger. Cell viability was not compromised. This effect of SNP was concomitant with a moderate reduction of cellular ATP content, an increase in lipid peroxidation, and an increase in cellular cGMP levels. The antioxidant superoxide dismutase (SOD) significantly attenuated the effect of SNP on cellular ATP content and blunted the increase in Papp caused by SNP. A soluble guanylate cyclase inhibitor did not affect the effect of SNP on the Papp.
NO enhances the paracellular permeability of OK cells possibly via mechanisms involving decreases in cellular ATP content.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10354270</pmid><doi>10.1046/j.1523-1755.1999.00465.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0085-2538 |
| ispartof | Kidney international, 1999-06, Vol.55 (6), p.2215-2223 |
| issn | 0085-2538 1523-1755 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69799597 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - metabolism Animals antioxidants Antioxidants - pharmacology Biological and medical sciences Cell Membrane Permeability - drug effects Cell Survival - drug effects Cells, Cultured Cyclic GMP - metabolism cytotoxicity Investigative techniques, diagnostic techniques (general aspects) Kidney Tubules, Proximal - cytology Kidney Tubules, Proximal - drug effects Kidney Tubules, Proximal - metabolism L-Lactate Dehydrogenase - metabolism Lipid Peroxidation - drug effects Mannitol - metabolism Medical sciences Nitric Oxide - metabolism Nitric Oxide Donors - pharmacology Nitroprusside - pharmacology Opossums Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques proximal tubule Superoxide Dismutase - pharmacology tight junctions transport Urinary system |
| title | Nitric oxide enhances paracellular permeability of opossum kidney cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T20%3A48%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nitric%20oxide%20enhances%20paracellular%20permeability%20of%20opossum%20kidney%20cells&rft.jtitle=Kidney%20international&rft.au=Liang,%20Mingyu&rft.date=1999-06-01&rft.volume=55&rft.issue=6&rft.spage=2215&rft.epage=2223&rft.pages=2215-2223&rft.issn=0085-2538&rft.eissn=1523-1755&rft.coden=KDYIA5&rft_id=info:doi/10.1046/j.1523-1755.1999.00465.x&rft_dat=%3Cproquest_cross%3E69799597%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69799597&rft_id=info:pmid/10354270&rft_els_id=S0085253815461848&rfr_iscdi=true |