Purification and characterization of an ADP-dependent phosphofructokinase from Thermococcus zilligii

The ADP-dependent phosphofructokinase (PFK) from Thermococcus zilligii has been purified 950 fold; it had a specific activity of 190 Umg(-1). The enzyme required Mg2+ ions for optimal activity and was specific for ADP. The forward reaction kinetics were hyperbolic for both cosubstrates (pH optimum o...

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Veröffentlicht in:	Extremophiles : life under extreme conditions 1999-05, Vol.3 (2), p.121-129
Hauptverfasser:	RONIMUS, R. S, KONING, J, MORGAN, H. W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - metabolism Bacteriology Biological and medical sciences Cations Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Metabolism. Enzymes Microbiology Molecular Weight Phosphofructokinase-1 - isolation & purification Phosphofructokinase-1 - metabolism Space life sciences Thermococcus - enzymology
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