N-glycans are not the signal for apical sorting of corticosteroid binding globulin in MDCK cells
It has been suggested that N-glycans act as a general sorting signal for secretory proteins in MDCK cells [Scheiffele et al. (1995) Nature 378, 96–98]. Human corticosteroid binding globulin contains six consensus sites for N-glycosylation and is known to be secreted to the apical side of MDCK cells....
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| Veröffentlicht in: | FEBS letters 1999-05, Vol.451 (1), p.19-22 |
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| container_title | FEBS letters |
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| creator | Larsen, Jakob E. Avvakumov, George V. Hammond, Geoffrey L. Vogel, Lotte K. |
| description | It has been suggested that
N-glycans act as a general sorting signal for secretory proteins in MDCK cells [Scheiffele et al. (1995) Nature 378, 96–98]. Human corticosteroid binding globulin contains six consensus sites for
N-glycosylation and is known to be secreted to the apical side of MDCK cells. Our results show that wild-type corticosteroid binding globulin is
N-glycosylated when it is recombinantly expressed in MDCK cells. Six mutants, each lacking one of the
N-glycosylation sites, and a mutant lacking all six
N-glycosylation sites were also secreted to the apical side of MDCK cells in a polarized manner. Thus, the
N-glycans on corticosteroid binding globulin do not act as an apical sorting signal in MDCK cells. |
| doi_str_mv | 10.1016/S0014-5793(99)00526-8 |
| format | Article |
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N-glycans act as a general sorting signal for secretory proteins in MDCK cells [Scheiffele et al. (1995) Nature 378, 96–98]. Human corticosteroid binding globulin contains six consensus sites for
N-glycosylation and is known to be secreted to the apical side of MDCK cells. Our results show that wild-type corticosteroid binding globulin is
N-glycosylated when it is recombinantly expressed in MDCK cells. Six mutants, each lacking one of the
N-glycosylation sites, and a mutant lacking all six
N-glycosylation sites were also secreted to the apical side of MDCK cells in a polarized manner. Thus, the
N-glycans on corticosteroid binding globulin do not act as an apical sorting signal in MDCK cells.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)00526-8</identifier><identifier>PMID: 10356976</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Apical secretion ; Cell Line ; Corticosteroid binding globulin ; Dogs ; Glycosylation ; Humans ; MDCK cell ; N-glycosylation ; Polysaccharides - metabolism ; Rabbits ; Transcortin - metabolism</subject><ispartof>FEBS letters, 1999-05, Vol.451 (1), p.19-22</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 451 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4268-ae56ea52dfe36edf7a323165573d112bd0f72196240ecf091b214c0ebcb919bb3</citedby><cites>FETCH-LOGICAL-c4268-ae56ea52dfe36edf7a323165573d112bd0f72196240ecf091b214c0ebcb919bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2899%2900526-8$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(99)00526-8$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,782,786,1419,1435,3554,27933,27934,45583,45584,46004,46418,46842</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10356976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Larsen, Jakob E.</creatorcontrib><creatorcontrib>Avvakumov, George V.</creatorcontrib><creatorcontrib>Hammond, Geoffrey L.</creatorcontrib><creatorcontrib>Vogel, Lotte K.</creatorcontrib><title>N-glycans are not the signal for apical sorting of corticosteroid binding globulin in MDCK cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>It has been suggested that
N-glycans act as a general sorting signal for secretory proteins in MDCK cells [Scheiffele et al. (1995) Nature 378, 96–98]. Human corticosteroid binding globulin contains six consensus sites for
N-glycosylation and is known to be secreted to the apical side of MDCK cells. Our results show that wild-type corticosteroid binding globulin is
N-glycosylated when it is recombinantly expressed in MDCK cells. Six mutants, each lacking one of the
N-glycosylation sites, and a mutant lacking all six
N-glycosylation sites were also secreted to the apical side of MDCK cells in a polarized manner. Thus, the
N-glycans on corticosteroid binding globulin do not act as an apical sorting signal in MDCK cells.</description><subject>Animals</subject><subject>Apical secretion</subject><subject>Cell Line</subject><subject>Corticosteroid binding globulin</subject><subject>Dogs</subject><subject>Glycosylation</subject><subject>Humans</subject><subject>MDCK cell</subject><subject>N-glycosylation</subject><subject>Polysaccharides - metabolism</subject><subject>Rabbits</subject><subject>Transcortin - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1P3DAQhi1UBMvHT6DyqSqHgD8SJz4huuyWiqU9AGfjj8nWVTZe7GzR_vsmG1RxayVLnvG88874QeiMkgtKqLh8IITmWVFK_lnKc0IKJrJqD01oVfKM56L6gCZ_JYfoKKVfpM8rKg_QISW8ELIUE_T8PVs2W6vbhHUE3IYOdz8BJ79sdYPrELFee9uHKcTOt0scamyH0IbUQQzeYeNbN1SWTTCbxre4P_c30ztsoWnSCdqvdZPg9O0-Rk_z2eP0Nlv8-Ppter3IbM5ElWkoBOiCuRq4AFeXmjNORVGU3FHKjCN1yagULCdgayKpYTS3BIw1kkpj-DH6NPquY3jZQOrUyqdhA91C2CTVf1dKVvJeWIxCG0NKEWq1jn6l41ZRoga0aodWDdyUlGqHVlV938e3ARuzAveua2TZC25HwatvYPt_rmo--8J2laEg5e55mHU1WkFP7LeHqJL10FpwPoLtlAv-H9v-AV9CnOA</recordid><startdate>19990514</startdate><enddate>19990514</enddate><creator>Larsen, Jakob E.</creator><creator>Avvakumov, George V.</creator><creator>Hammond, Geoffrey L.</creator><creator>Vogel, Lotte K.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990514</creationdate><title>N-glycans are not the signal for apical sorting of corticosteroid binding globulin in MDCK cells</title><author>Larsen, Jakob E. ; Avvakumov, George V. ; Hammond, Geoffrey L. ; Vogel, Lotte K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4268-ae56ea52dfe36edf7a323165573d112bd0f72196240ecf091b214c0ebcb919bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Apical secretion</topic><topic>Cell Line</topic><topic>Corticosteroid binding globulin</topic><topic>Dogs</topic><topic>Glycosylation</topic><topic>Humans</topic><topic>MDCK cell</topic><topic>N-glycosylation</topic><topic>Polysaccharides - metabolism</topic><topic>Rabbits</topic><topic>Transcortin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Larsen, Jakob E.</creatorcontrib><creatorcontrib>Avvakumov, George V.</creatorcontrib><creatorcontrib>Hammond, Geoffrey L.</creatorcontrib><creatorcontrib>Vogel, Lotte K.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Larsen, Jakob E.</au><au>Avvakumov, George V.</au><au>Hammond, Geoffrey L.</au><au>Vogel, Lotte K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-glycans are not the signal for apical sorting of corticosteroid binding globulin in MDCK cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-05-14</date><risdate>1999</risdate><volume>451</volume><issue>1</issue><spage>19</spage><epage>22</epage><pages>19-22</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>It has been suggested that
N-glycans act as a general sorting signal for secretory proteins in MDCK cells [Scheiffele et al. (1995) Nature 378, 96–98]. Human corticosteroid binding globulin contains six consensus sites for
N-glycosylation and is known to be secreted to the apical side of MDCK cells. Our results show that wild-type corticosteroid binding globulin is
N-glycosylated when it is recombinantly expressed in MDCK cells. Six mutants, each lacking one of the
N-glycosylation sites, and a mutant lacking all six
N-glycosylation sites were also secreted to the apical side of MDCK cells in a polarized manner. Thus, the
N-glycans on corticosteroid binding globulin do not act as an apical sorting signal in MDCK cells.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10356976</pmid><doi>10.1016/S0014-5793(99)00526-8</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Animals Apical secretion Cell Line Corticosteroid binding globulin Dogs Glycosylation Humans MDCK cell N-glycosylation Polysaccharides - metabolism Rabbits Transcortin - metabolism |
| title | N-glycans are not the signal for apical sorting of corticosteroid binding globulin in MDCK cells |
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