Elevated CO2 alters community-level physiological profiles and enzyme activities in alpine grassland
Plots of an alpine grassland in the Swiss Alps were treated with elevated (680 microl l(-1)) and ambient CO2 (355 microl l(-1)) in open top chambers (OTC). Several plots were also treated with NPK-fertilizer. Community level physiological profiles (CLPPs) of the soil bacteria were examined by Biolog...
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| Veröffentlicht in: | Journal of microbiological methods 1999-05, Vol.36 (1-2), p.35-43 |
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| creator | MAYR, C MILLER, M INSAM, H |
| description | Plots of an alpine grassland in the Swiss Alps were treated with elevated (680 microl l(-1)) and ambient CO2 (355 microl l(-1)) in open top chambers (OTC). Several plots were also treated with NPK-fertilizer. Community level physiological profiles (CLPPs) of the soil bacteria were examined by Biolog GN microplates and enzyme activities were determined through the release of methylumbelliferyl (MUF) and methylcoumarin (MC) from MUF- or MC-labelled substrates. A canonical discriminant analysis (CDA) followed by multivariate analysis of variance showed a significant effect of elevated CO2 on the CLPPs both under fertilized and unfertilized conditions. Further, the installation of the OTCs caused significant shifts in the CLPPs (chamber effect). Of the four enzyme activities tested, the beta-D-cellobiohydrolase (CELase) and N-acetyl-beta-D-glucosaminidase (NAGase) activity were enhanced under elevated CO2. L-Leucin-7-aminopeptidase (APEase) activity decreased, when the plots received fertilizer. Beta-D-glucosidase (GLUase) remained unaffected. The results suggest effects of elevated CO2 on specific microbial activities even under low mineral nutrient conditions and when bulk parameters like microbial biomass or respiration, which have been investigated on the same site, remain unaffected. The observed medium-term changes point at possible long-term consequences for the ecosystem that may not be specified yet. |
| doi_str_mv | 10.1016/S0167-7012(99)00009-3 |
| format | Article |
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Several plots were also treated with NPK-fertilizer. Community level physiological profiles (CLPPs) of the soil bacteria were examined by Biolog GN microplates and enzyme activities were determined through the release of methylumbelliferyl (MUF) and methylcoumarin (MC) from MUF- or MC-labelled substrates. A canonical discriminant analysis (CDA) followed by multivariate analysis of variance showed a significant effect of elevated CO2 on the CLPPs both under fertilized and unfertilized conditions. Further, the installation of the OTCs caused significant shifts in the CLPPs (chamber effect). Of the four enzyme activities tested, the beta-D-cellobiohydrolase (CELase) and N-acetyl-beta-D-glucosaminidase (NAGase) activity were enhanced under elevated CO2. L-Leucin-7-aminopeptidase (APEase) activity decreased, when the plots received fertilizer. Beta-D-glucosidase (GLUase) remained unaffected. The results suggest effects of elevated CO2 on specific microbial activities even under low mineral nutrient conditions and when bulk parameters like microbial biomass or respiration, which have been investigated on the same site, remain unaffected. The observed medium-term changes point at possible long-term consequences for the ecosystem that may not be specified yet.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/S0167-7012(99)00009-3</identifier><identifier>PMID: 10353798</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier Science</publisher><subject>Acetylglucosaminidase - metabolism ; Agronomy. 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Several plots were also treated with NPK-fertilizer. Community level physiological profiles (CLPPs) of the soil bacteria were examined by Biolog GN microplates and enzyme activities were determined through the release of methylumbelliferyl (MUF) and methylcoumarin (MC) from MUF- or MC-labelled substrates. A canonical discriminant analysis (CDA) followed by multivariate analysis of variance showed a significant effect of elevated CO2 on the CLPPs both under fertilized and unfertilized conditions. Further, the installation of the OTCs caused significant shifts in the CLPPs (chamber effect). Of the four enzyme activities tested, the beta-D-cellobiohydrolase (CELase) and N-acetyl-beta-D-glucosaminidase (NAGase) activity were enhanced under elevated CO2. L-Leucin-7-aminopeptidase (APEase) activity decreased, when the plots received fertilizer. Beta-D-glucosidase (GLUase) remained unaffected. The results suggest effects of elevated CO2 on specific microbial activities even under low mineral nutrient conditions and when bulk parameters like microbial biomass or respiration, which have been investigated on the same site, remain unaffected. The observed medium-term changes point at possible long-term consequences for the ecosystem that may not be specified yet.</description><subject>Acetylglucosaminidase - metabolism</subject><subject>Agronomy. 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Psychology</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>Poaceae</subject><subject>Soil</subject><subject>Soil Microbiology</subject><subject>Soil science</subject><subject>Switzerland</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLxDAQgIMouj5-gtKDiB6qSadpkqMsvkDwoJ7DbDLVSB9r0xXWX290FzWHCZP5ZjJ8jB0Kfi64qC4eU1C54qI4NeaMp2Ny2GAToVWRa5Bmk01-kR22G-Mb50JCqbfZjuAgQRk9Yf6qoQ8cyWfThyLDZqQhZq5v20UXxmWeitRk89dlDH3TvwSHKRv6OjQUM-x8Rt3nsqUM3Rg-whjSa-jSmHnoKHsZMMYmUftsq8Ym0sH63mPP11dP09v8_uHmbnp5nzuAcsxrV1aFVoDgAY1HErMKPHeuRCURtPA0g7r2iKhLAUYJo6WTUqmKqOYG9tjJam5a8X1BcbRtiI6atAP1i2gro4wRpkigXIFu6GMcqLbzIbQ4LK3g9luv_dFrv91ZY-yPXgup72j9wWLWkv_XtfKZgOM1gDG5qgfsXIh_nOaFhAq-AOyShH0</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>MAYR, C</creator><creator>MILLER, M</creator><creator>INSAM, H</creator><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Elevated CO2 alters community-level physiological profiles and enzyme activities in alpine grassland</title><author>MAYR, C ; MILLER, M ; INSAM, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-fc462873a3d3a9dae1b63d0cc4a75a381deb3ffdaaa8413971985c55776eef093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acetylglucosaminidase - metabolism</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Aminopeptidases - metabolism</topic><topic>Animal, plant and microbial ecology</topic><topic>beta-Glucosidase - metabolism</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Carbon Dioxide - metabolism</topic><topic>Cellulase - metabolism</topic><topic>Cellulose 1,4-beta-Cellobiosidase</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Coumarins - metabolism</topic><topic>Ecosystem</topic><topic>Fertilizers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Poaceae</topic><topic>Soil</topic><topic>Soil Microbiology</topic><topic>Soil science</topic><topic>Switzerland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAYR, C</creatorcontrib><creatorcontrib>MILLER, M</creatorcontrib><creatorcontrib>INSAM, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAYR, C</au><au>MILLER, M</au><au>INSAM, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevated CO2 alters community-level physiological profiles and enzyme activities in alpine grassland</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>36</volume><issue>1-2</issue><spage>35</spage><epage>43</epage><pages>35-43</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>Plots of an alpine grassland in the Swiss Alps were treated with elevated (680 microl l(-1)) and ambient CO2 (355 microl l(-1)) in open top chambers (OTC). 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The results suggest effects of elevated CO2 on specific microbial activities even under low mineral nutrient conditions and when bulk parameters like microbial biomass or respiration, which have been investigated on the same site, remain unaffected. The observed medium-term changes point at possible long-term consequences for the ecosystem that may not be specified yet.</abstract><cop>Shannon</cop><pub>Elsevier Science</pub><pmid>10353798</pmid><doi>10.1016/S0167-7012(99)00009-3</doi><tpages>9</tpages></addata></record> |
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| subjects | Acetylglucosaminidase - metabolism Agronomy. Soil science and plant productions Aminopeptidases - metabolism Animal, plant and microbial ecology beta-Glucosidase - metabolism Biochemistry and biology Biological and medical sciences Carbon Dioxide - metabolism Cellulase - metabolism Cellulose 1,4-beta-Cellobiosidase Chemical, physicochemical, biochemical and biological properties Coumarins - metabolism Ecosystem Fertilizers Fundamental and applied biological sciences. Psychology Microbial ecology Microbiology Physics, chemistry, biochemistry and biology of agricultural and forest soils Poaceae Soil Soil Microbiology Soil science Switzerland |
| title | Elevated CO2 alters community-level physiological profiles and enzyme activities in alpine grassland |
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