Absorption of IgG does not enhance toxoplasma IgM and IgA immunoblotting
1 Microbiology Department, Raigmore Hospital NHS Trust, Inverness IV2 3UJ 2 Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD 3 Corresponding author: Dr J. M. W. Chatterton. Received March 19, 1998 Accepted September 29, 1998 Total IgG, IgM and IgA levels and...
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| Veröffentlicht in: | Journal of medical microbiology 1999-06, Vol.48 (6), p.593-595, Article 593 |
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| container_issue | 6 |
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| container_title | Journal of medical microbiology |
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| creator | CHATTERTON, JEAN M. W WALKINGSHAW, D JOSS, A. W. L PENNINGTON, T. H HO-YEN, D. O |
| description | 1 Microbiology Department, Raigmore Hospital NHS Trust, Inverness IV2 3UJ
2 Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
3 Corresponding author: Dr J. M. W. Chatterton.
Received March 19, 1998
Accepted September 29, 1998
Total IgG, IgM and IgA levels and toxoplasma IgG. IgM and IgA immunoblotting patterns were assayed in 10 sera before and after IgG absorption with Protein G -Sepharose 4. Removal of IgG (mean reduction 96%) was accompanied by a significant reduction in the level of IgM (mean reduction 56%) and IgA (mean reduction 53%) in nine of the 10 sera. The absorbed supernates showed fewer and weaker IgM bands in five sera, but IgA immunoblotting patterns were unaffected by absorption. There was no benefit in removing IgG in toxoplasma IgM and IgA immunoblotting. |
| doi_str_mv | 10.1099/00222615-48-6-593 |
| format | Article |
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2 Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
3 Corresponding author: Dr J. M. W. Chatterton.
Received March 19, 1998
Accepted September 29, 1998
Total IgG, IgM and IgA levels and toxoplasma IgG. IgM and IgA immunoblotting patterns were assayed in 10 sera before and after IgG absorption with Protein G -Sepharose 4. Removal of IgG (mean reduction 96%) was accompanied by a significant reduction in the level of IgM (mean reduction 56%) and IgA (mean reduction 53%) in nine of the 10 sera. The absorbed supernates showed fewer and weaker IgM bands in five sera, but IgA immunoblotting patterns were unaffected by absorption. There was no benefit in removing IgG in toxoplasma IgM and IgA immunoblotting.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/00222615-48-6-593</identifier><identifier>PMID: 10359309</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Absorption ; Animals ; Antibodies, Protozoan - analysis ; Antibodies, Protozoan - metabolism ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; General aspects and techniques ; Human protozoal diseases ; Humans ; Immunoblotting ; Immunoglobulin A - analysis ; Immunoglobulin G - analysis ; Immunoglobulin G - metabolism ; Immunoglobulin M - analysis ; Infectious diseases ; Medical sciences ; Nerve Tissue Proteins - metabolism ; Parasitic diseases ; Protozoa ; Protozoal diseases ; Sepharose - metabolism ; Toxoplasma ; Toxoplasma - immunology ; Toxoplasmosis</subject><ispartof>Journal of medical microbiology, 1999-06, Vol.48 (6), p.593-595, Article 593</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-e0eecbe6311fa83b83c3204ffbbd34b0635bb38afaee5b4e013ec6c2a6564f2d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,3744,3745,23928,23929,25138,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1847063$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10359309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHATTERTON, JEAN M. W</creatorcontrib><creatorcontrib>WALKINGSHAW, D</creatorcontrib><creatorcontrib>JOSS, A. W. L</creatorcontrib><creatorcontrib>PENNINGTON, T. H</creatorcontrib><creatorcontrib>HO-YEN, D. O</creatorcontrib><title>Absorption of IgG does not enhance toxoplasma IgM and IgA immunoblotting</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>1 Microbiology Department, Raigmore Hospital NHS Trust, Inverness IV2 3UJ
2 Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
3 Corresponding author: Dr J. M. W. Chatterton.
Received March 19, 1998
Accepted September 29, 1998
Total IgG, IgM and IgA levels and toxoplasma IgG. IgM and IgA immunoblotting patterns were assayed in 10 sera before and after IgG absorption with Protein G -Sepharose 4. Removal of IgG (mean reduction 96%) was accompanied by a significant reduction in the level of IgM (mean reduction 56%) and IgA (mean reduction 53%) in nine of the 10 sera. The absorbed supernates showed fewer and weaker IgM bands in five sera, but IgA immunoblotting patterns were unaffected by absorption. There was no benefit in removing IgG in toxoplasma IgM and IgA immunoblotting.</description><subject>Absorption</subject><subject>Animals</subject><subject>Antibodies, Protozoan - analysis</subject><subject>Antibodies, Protozoan - metabolism</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects and techniques</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin G - metabolism</subject><subject>Immunoglobulin M - analysis</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Parasitic diseases</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Sepharose - metabolism</subject><subject>Toxoplasma</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasmosis</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTFvFDEQhS1ERI7AD6BBWyCoFuy112uXpwiSSEFpQm3Z3vGdo7V92D4B_x6f7gKIAqop5ntvZt4g9Irg9wRL-QHjYRg4GXsmet6Pkj5BK8Im2o-csadodej3B-AcPS_lAWMyUSqfoXOCaaOxXKHrtSkp76pPsUuuu9lcdXOC0sVUO4hbHS10NX1Pu0WXoFv_c6fj3Oq68yHsYzJLqtXHzQt05vRS4OWpXqAvnz7eX173t3dXN5fr295SIWsPGMAa4JQQpwU1glo6YOacMTNlBnM6GkOFdhpgNAwwoWC5HTRvN7lhphfo7dF3l9PXPZSqgi8WlkVHSPuiuJyk4FL-FyTTwEcxsgaSI2hzKiWDU7vsg84_FMHqkLN6zFkxobhqyTXN65P53gSY_1Acg23AmxOgi9WLyy1JX35zgk3t1oZNf822vurDN2rWfvnnBu-Oyq3fbL_5DGoDMfjmY3xSDyH8In8CKbqkUQ</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>CHATTERTON, JEAN M. 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O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-e0eecbe6311fa83b83c3204ffbbd34b0635bb38afaee5b4e013ec6c2a6564f2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Absorption</topic><topic>Animals</topic><topic>Antibodies, Protozoan - analysis</topic><topic>Antibodies, Protozoan - metabolism</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects and techniques</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin G - metabolism</topic><topic>Immunoglobulin M - analysis</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Parasitic diseases</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><topic>Sepharose - metabolism</topic><topic>Toxoplasma</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasmosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHATTERTON, JEAN M. W</creatorcontrib><creatorcontrib>WALKINGSHAW, D</creatorcontrib><creatorcontrib>JOSS, A. W. L</creatorcontrib><creatorcontrib>PENNINGTON, T. H</creatorcontrib><creatorcontrib>HO-YEN, D. 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O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absorption of IgG does not enhance toxoplasma IgM and IgA immunoblotting</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>48</volume><issue>6</issue><spage>593</spage><epage>595</epage><pages>593-595</pages><artnum>593</artnum><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>1 Microbiology Department, Raigmore Hospital NHS Trust, Inverness IV2 3UJ
2 Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
3 Corresponding author: Dr J. M. W. Chatterton.
Received March 19, 1998
Accepted September 29, 1998
Total IgG, IgM and IgA levels and toxoplasma IgG. IgM and IgA immunoblotting patterns were assayed in 10 sera before and after IgG absorption with Protein G -Sepharose 4. Removal of IgG (mean reduction 96%) was accompanied by a significant reduction in the level of IgM (mean reduction 56%) and IgA (mean reduction 53%) in nine of the 10 sera. The absorbed supernates showed fewer and weaker IgM bands in five sera, but IgA immunoblotting patterns were unaffected by absorption. There was no benefit in removing IgG in toxoplasma IgM and IgA immunoblotting.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>10359309</pmid><doi>10.1099/00222615-48-6-593</doi><tpages>3</tpages></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Absorption Animals Antibodies, Protozoan - analysis Antibodies, Protozoan - metabolism Biological and medical sciences Fundamental and applied biological sciences. Psychology General aspects and techniques Human protozoal diseases Humans Immunoblotting Immunoglobulin A - analysis Immunoglobulin G - analysis Immunoglobulin G - metabolism Immunoglobulin M - analysis Infectious diseases Medical sciences Nerve Tissue Proteins - metabolism Parasitic diseases Protozoa Protozoal diseases Sepharose - metabolism Toxoplasma Toxoplasma - immunology Toxoplasmosis |
| title | Absorption of IgG does not enhance toxoplasma IgM and IgA immunoblotting |
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