Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain
Hyperammonemia in the brain leads to poorly understood alterations of nitric oxide (NO) synthesis. Arginine, the substrate of nitric oxide synthases, might be recycled from the citrulline produced with NO by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL). The regulation of AS and...
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| Veröffentlicht in: | Neuroscience letters 1999-05, Vol.266 (2), p.89-92 |
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| container_title | Neuroscience letters |
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| creator | Braissant, Olivier Honegger, Paul Loup, Marc Iwase, Katsuro Takiguchi, Masaki Bachmann, Claude |
| description | Hyperammonemia in the brain leads to poorly understood alterations of nitric oxide (NO) synthesis. Arginine, the substrate of nitric oxide synthases, might be recycled from the citrulline produced with NO by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL). The regulation of AS and AL genes during hyperammonemia is unknown in the brain. We used brain cell aggregates cultured from dissociated telencephalic cortex of rat embryos to analyze the regulation of AS and AL genes in hyperammonemia. Using RNase protection assay and non-radioactive in situ hybridization on aggregate cryosections, we show that both AS and AL genes are induced in astrocytes but not in neurons of aggregates exposed to 5 mM NH
4Cl. Our work suggests that the hyperammonemic brain might increase its recycling of citrulline to arginine. |
| doi_str_mv | 10.1016/S0304-3940(99)00274-8 |
| format | Article |
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4Cl. Our work suggests that the hyperammonemic brain might increase its recycling of citrulline to arginine.</description><subject>Ammonia - blood</subject><subject>Animals</subject><subject>Arginine</subject><subject>Argininosuccinate</subject><subject>Argininosuccinate Lyase - genetics</subject><subject>Argininosuccinate Synthase - genetics</subject><subject>argininosuccinate synthetase</subject><subject>Astrocyte</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>Brain - cytology</subject><subject>Brain - embryology</subject><subject>Brain - metabolism</subject><subject>Cell Aggregation - physiology</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>citrulline</subject><subject>Citrulline-NO cycle</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Hyperammonemia</subject><subject>Rats</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1TAUhoMozjj6E5QsRHRRTZqvxo3IoI4w4EJdh9P0pEba9Jq0wv0P_mhz517UhTCrLM5z3rych5DHnL3kjOtXn5lgshFWsufWvmCsNbLp7pBz3pm2Mda0d8n5H-SMPCjlO2NMcSXvkzPOhBJCyHPy62q_wwzzvCScI7ymGcdtgjUuiS6BQh5jimkpm_cxwYq07NP6DVcoSCEN_wGm_WE2YsJCY6IwjjWyBqaRepwm6rdp3XId1vhQgyaaYaV9hpgeknsBpoKPTu8F-fr-3ZfLq-b604ePl2-vGy91tzZaGuDKtNaYttdGG-iCCpJL1XOUg9KDAhbafhDeqhAEdkKhsopbzXs79OKCPDvm7vLyY8OyujmWQzlIuGzFaWss50LfCnIjagltKqiOoM9LKRmD2-U4Q947ztzBl7vx5Q4ynLXuxpfr6t6T0wdbP-Pwz9ZRUAWengAoHqaQIflY_nK1qDa2Ym-OGNaz_YyYXfERk8chZvSrG5Z4S5Pf7ui0mw</recordid><startdate>19990507</startdate><enddate>19990507</enddate><creator>Braissant, Olivier</creator><creator>Honegger, Paul</creator><creator>Loup, Marc</creator><creator>Iwase, Katsuro</creator><creator>Takiguchi, Masaki</creator><creator>Bachmann, Claude</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19990507</creationdate><title>Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain</title><author>Braissant, Olivier ; Honegger, Paul ; Loup, Marc ; Iwase, Katsuro ; Takiguchi, Masaki ; Bachmann, Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-647a15729772b6767a8f5f4145b1e4d56d5a0f2bd3c95ff3e835e5951961b9db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Ammonia - blood</topic><topic>Animals</topic><topic>Arginine</topic><topic>Argininosuccinate</topic><topic>Argininosuccinate Lyase - genetics</topic><topic>Argininosuccinate Synthase - genetics</topic><topic>argininosuccinate synthetase</topic><topic>Astrocyte</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>Brain - cytology</topic><topic>Brain - embryology</topic><topic>Brain - metabolism</topic><topic>Cell Aggregation - physiology</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>citrulline</topic><topic>Citrulline-NO cycle</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Hyperammonemia</topic><topic>Rats</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Braissant, Olivier</creatorcontrib><creatorcontrib>Honegger, Paul</creatorcontrib><creatorcontrib>Loup, Marc</creatorcontrib><creatorcontrib>Iwase, Katsuro</creatorcontrib><creatorcontrib>Takiguchi, Masaki</creatorcontrib><creatorcontrib>Bachmann, Claude</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Braissant, Olivier</au><au>Honegger, Paul</au><au>Loup, Marc</au><au>Iwase, Katsuro</au><au>Takiguchi, Masaki</au><au>Bachmann, Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1999-05-07</date><risdate>1999</risdate><volume>266</volume><issue>2</issue><spage>89</spage><epage>92</epage><pages>89-92</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Hyperammonemia in the brain leads to poorly understood alterations of nitric oxide (NO) synthesis. Arginine, the substrate of nitric oxide synthases, might be recycled from the citrulline produced with NO by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL). The regulation of AS and AL genes during hyperammonemia is unknown in the brain. We used brain cell aggregates cultured from dissociated telencephalic cortex of rat embryos to analyze the regulation of AS and AL genes in hyperammonemia. Using RNase protection assay and non-radioactive in situ hybridization on aggregate cryosections, we show that both AS and AL genes are induced in astrocytes but not in neurons of aggregates exposed to 5 mM NH
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| ispartof | Neuroscience letters, 1999-05, Vol.266 (2), p.89-92 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Ammonia - blood Animals Arginine Argininosuccinate Argininosuccinate Lyase - genetics Argininosuccinate Synthase - genetics argininosuccinate synthetase Astrocyte Biochemistry and metabolism Biological and medical sciences Brain Brain - cytology Brain - embryology Brain - metabolism Cell Aggregation - physiology Cells, Cultured Central nervous system citrulline Citrulline-NO cycle Embryonic and Fetal Development - physiology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - physiology Gene Expression Regulation, Enzymologic - physiology Hyperammonemia Rats Vertebrates: nervous system and sense organs |
| title | Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain |
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