[15] READS: A method for display of 3′-end fragments of restriction enzyme-digested cDNAs for analysis of differential gene expression

This chapter discusses a gel-based method for analysis of differential gene expression called READS 2 or restriction endonucleolytic analysis of differentially expressed sequences. This method uses stringent polymerase chain reaction (PCR) conditions to amplify only the extreme Y-end fragment of a r...

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Veröffentlicht in:	Methods in Enzymology 1999, Vol.303, p.258-272
Hauptverfasser:	Prashar, Yatindra, Weissman, Sherman M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cell Cycle DNA Primers DNA Restriction Enzymes DNA, Complementary DNA, Ribosomal - genetics Humans Jurkat Cells Oligodeoxyribonucleotides Restriction Mapping - methods RNA, Ribosomal, 18S - genetics RNA, Ribosomal, 28S - genetics
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