Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast
We identified a new, unique upstream activating sequence (5′-GGTGGCAAA-3′) in the promoters of 26 out of the 32 proteasomal yeast genes characterized to date, which we propose to call proteasome-associated control element. By using the one-hybrid method, we show that the factor binding to the protea...
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| Veröffentlicht in: | FEBS letters 1999-04, Vol.450 (1), p.27-34 |
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| creator | Mannhaupt, Gertrud Schnall, Ralf Karpov, Vadim Vetter, Irene Feldmann, Horst |
| description | We identified a new, unique upstream activating sequence (5′-GGTGGCAAA-3′) in the promoters of 26 out of the 32 proteasomal yeast genes characterized to date, which we propose to call proteasome-associated control element. By using the one-hybrid method, we show that the factor binding to the proteasome-associated control element is Rpn4p, a protein containing a C2H2-type finger motif and two acidic domains. Electrophoretic mobility shift assays using proteasome-associated control element sequences from two regulatory proteasomal genes confirmed specific binding of purified Rpn4p to these sequences. The role of Rpn4p to function as a transregulator in yeast is corroborated by its ability of stimulating proteasome-associated control element-driven
lacZ expression and by experiments using the
RPT4 and
RPT6 gene promoters coupled to the bacterial
cat gene as a reporter. Additionally, we found the proteasome-associated control element to occur in a number of promoters to genes which are related to the ubiquitin-proteasome pathway in yeast. |
| doi_str_mv | 10.1016/S0014-5793(99)00467-6 |
| format | Article |
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lacZ expression and by experiments using the
RPT4 and
RPT6 gene promoters coupled to the bacterial
cat gene as a reporter. Additionally, we found the proteasome-associated control element to occur in a number of promoters to genes which are related to the ubiquitin-proteasome pathway in yeast.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)00467-6</identifier><identifier>PMID: 10350051</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>26S proteasome ; Amino Acid Sequence ; Binding Sites ; Cloning, Molecular ; Cysteine Endopeptidases - genetics ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - genetics ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Genes, Reporter ; Molecular Sequence Data ; Multienzyme Complexes - genetics ; Oligodeoxyribonucleotides - genetics ; Promoter Regions, Genetic ; Proteasome Endopeptidase Complex ; Regulatory Sequences, Nucleic Acid ; Rpn4p ; Saccharomyces cerevisiae Proteins ; Transcription factor ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Upstream activating sequences element ; Yeast ; Yeasts - enzymology ; Zinc Fingers - genetics</subject><ispartof>FEBS letters, 1999-04, Vol.450 (1), p.27-34</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 450 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5916-bec0382667cf65c1dcd1cda3746e23563b1692b24bb89300c1cb01971d4e2d53</citedby><cites>FETCH-LOGICAL-c5916-bec0382667cf65c1dcd1cda3746e23563b1692b24bb89300c1cb01971d4e2d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2899%2900467-6$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(99)00467-6$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,1432,3548,27923,27924,45573,45574,45994,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10350051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mannhaupt, Gertrud</creatorcontrib><creatorcontrib>Schnall, Ralf</creatorcontrib><creatorcontrib>Karpov, Vadim</creatorcontrib><creatorcontrib>Vetter, Irene</creatorcontrib><creatorcontrib>Feldmann, Horst</creatorcontrib><title>Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We identified a new, unique upstream activating sequence (5′-GGTGGCAAA-3′) in the promoters of 26 out of the 32 proteasomal yeast genes characterized to date, which we propose to call proteasome-associated control element. By using the one-hybrid method, we show that the factor binding to the proteasome-associated control element is Rpn4p, a protein containing a C2H2-type finger motif and two acidic domains. Electrophoretic mobility shift assays using proteasome-associated control element sequences from two regulatory proteasomal genes confirmed specific binding of purified Rpn4p to these sequences. The role of Rpn4p to function as a transregulator in yeast is corroborated by its ability of stimulating proteasome-associated control element-driven
lacZ expression and by experiments using the
RPT4 and
RPT6 gene promoters coupled to the bacterial
cat gene as a reporter. Additionally, we found the proteasome-associated control element to occur in a number of promoters to genes which are related to the ubiquitin-proteasome pathway in yeast.</description><subject>26S proteasome</subject><subject>Amino Acid Sequence</subject><subject>Binding Sites</subject><subject>Cloning, Molecular</subject><subject>Cysteine Endopeptidases - genetics</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Genes, Reporter</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - genetics</subject><subject>Oligodeoxyribonucleotides - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Proteasome Endopeptidase Complex</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Rpn4p</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Transcription factor</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Upstream activating sequences element</subject><subject>Yeast</subject><subject>Yeasts - enzymology</subject><subject>Zinc Fingers - genetics</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF9r1TAYh4Mo7mz6EZRciQOrSdOmzZVshzM3GChu9yFN3s5Im9Qk1Z1Lv7np6RDvHARC8nvePzwIvaLkPSWUf7ghhFZF3Qj2VohTQireFPwJ2tC2YQWrePsUbf4iR-g4xu8kv1sqnqMjSlhNSE036PfXyVUTVjpFrPLBKSgXdbBTst7hPgc-4G6PO-uMdXc4efzlbLt7l1HnnRohp_4e9352Bs9TTAHUiH2PS36Dp-ATqOhHNWCVc5--Zf4OHERsHd7nLL1Az3o1RHj5cJ-g24vd7fayuP786Wp7dl3oWlBedKAJa0vOG93zWlOjDdVGsabiULKas45yUXZl1XWtYIRoqjtCRUNNBaWp2Ql6s7bNO_2YISY52qhhGJQDP0fJRSOyngWsV1AHH2OAXk7BjirsJSVyUS8P6uXiVQohD-olz3WvHwbM3Qjmn6rVdQYuV-CXHWD_uK7yYndeHpIlEOLwvcz6uLaCLOynhSCjtuA0GBtAJ2m8_c-2fwAicKcN</recordid><startdate>19990430</startdate><enddate>19990430</enddate><creator>Mannhaupt, Gertrud</creator><creator>Schnall, Ralf</creator><creator>Karpov, Vadim</creator><creator>Vetter, Irene</creator><creator>Feldmann, Horst</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990430</creationdate><title>Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast</title><author>Mannhaupt, Gertrud ; Schnall, Ralf ; Karpov, Vadim ; Vetter, Irene ; Feldmann, Horst</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5916-bec0382667cf65c1dcd1cda3746e23563b1692b24bb89300c1cb01971d4e2d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>26S proteasome</topic><topic>Amino Acid Sequence</topic><topic>Binding Sites</topic><topic>Cloning, Molecular</topic><topic>Cysteine Endopeptidases - genetics</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Genes, Reporter</topic><topic>Molecular Sequence Data</topic><topic>Multienzyme Complexes - genetics</topic><topic>Oligodeoxyribonucleotides - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Proteasome Endopeptidase Complex</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Rpn4p</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Transcription factor</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><topic>Upstream activating sequences element</topic><topic>Yeast</topic><topic>Yeasts - enzymology</topic><topic>Zinc Fingers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mannhaupt, Gertrud</creatorcontrib><creatorcontrib>Schnall, Ralf</creatorcontrib><creatorcontrib>Karpov, Vadim</creatorcontrib><creatorcontrib>Vetter, Irene</creatorcontrib><creatorcontrib>Feldmann, Horst</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mannhaupt, Gertrud</au><au>Schnall, Ralf</au><au>Karpov, Vadim</au><au>Vetter, Irene</au><au>Feldmann, Horst</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-04-30</date><risdate>1999</risdate><volume>450</volume><issue>1</issue><spage>27</spage><epage>34</epage><pages>27-34</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We identified a new, unique upstream activating sequence (5′-GGTGGCAAA-3′) in the promoters of 26 out of the 32 proteasomal yeast genes characterized to date, which we propose to call proteasome-associated control element. By using the one-hybrid method, we show that the factor binding to the proteasome-associated control element is Rpn4p, a protein containing a C2H2-type finger motif and two acidic domains. Electrophoretic mobility shift assays using proteasome-associated control element sequences from two regulatory proteasomal genes confirmed specific binding of purified Rpn4p to these sequences. The role of Rpn4p to function as a transregulator in yeast is corroborated by its ability of stimulating proteasome-associated control element-driven
lacZ expression and by experiments using the
RPT4 and
RPT6 gene promoters coupled to the bacterial
cat gene as a reporter. Additionally, we found the proteasome-associated control element to occur in a number of promoters to genes which are related to the ubiquitin-proteasome pathway in yeast.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10350051</pmid><doi>10.1016/S0014-5793(99)00467-6</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | 26S proteasome Amino Acid Sequence Binding Sites Cloning, Molecular Cysteine Endopeptidases - genetics DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics Gene Expression Regulation, Fungal Genes, Fungal Genes, Reporter Molecular Sequence Data Multienzyme Complexes - genetics Oligodeoxyribonucleotides - genetics Promoter Regions, Genetic Proteasome Endopeptidase Complex Regulatory Sequences, Nucleic Acid Rpn4p Saccharomyces cerevisiae Proteins Transcription factor Transcription Factors - chemistry Transcription Factors - genetics Upstream activating sequences element Yeast Yeasts - enzymology Zinc Fingers - genetics |
| title | Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast |
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