Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit
Structural analysis of the 16 S rRNA in the 30 S subunit and 70 S ribosome in the presence of ribosome-specific antibiotics was performed to determine whether they produced rRNA structural changes that might provide further insight to their action. An UV cross-linking procedure that determines the p...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1999-06, Vol.274 (23), p.16576-16581 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 16581 |
|---|---|
| container_issue | 23 |
| container_start_page | 16576 |
| container_title | The Journal of biological chemistry |
| container_volume | 274 |
| creator | Noah, J W Dolan, M A Babin, P Wollenzien, P |
| description | Structural analysis of the 16 S rRNA in the 30 S subunit and 70 S ribosome in the presence of ribosome-specific antibiotics
was performed to determine whether they produced rRNA structural changes that might provide further insight to their action.
An UV cross-linking procedure that determines the pattern and frequency of intramolecular 16 S RNA cross-links was used to
detect differences reflecting structural changes. Tetracycline and spectinomycin have specific effects detected by this assay.
The presence of tetracycline inhibits the cross-link C967ÃC1400 completely, increases the frequency of cross-link C1402Ã1501
twofold, and decreases the cross-link G894ÃU244 by one-half without affecting other cross-links. Spectinomycin reduces the
frequency of the cross-link C934ÃU1345 by 60% without affecting cross-linking at other sites. The structural changes occur
at concentrations at which the antibiotics exert their inhibitory effects. For spectinomycin, the apparent binding site and
the affected cross-linking site are distant in the secondary structure but are close in tertiary structure in several recent
models, indicating a localized effect. For tetracycline, the apparent binding sites are significantly separated in both the
secondary and the three-dimensional structures, suggesting a more regional effect. |
| doi_str_mv | 10.1074/jbc.274.23.16576 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69787907</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17238796</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-cfb8f457b8d297a0fefd7a4d792d4d5ffd173637014b78a4387b35bdf1bff93a3</originalsourceid><addsrcrecordid>eNqFkTtvFDEURi0EIktCT4VcILpZ_JrxuIyiBSJFRMomEp3lJ-NoZrzYHqGt-eNxMilCxW1ucc_3FfcA8AGjLUacfbnXZks42xK6xV3Lu1dgg1FPG9rin6_BBiGCG0Ha_gS8y_ke1WECvwUnGFHGCaEb8HfnvTMlw-jhrStJmaMZw-ygmi3cH-opzHE6mjDDOMMyuEqlElQ6wn1JiylLco_Zm6BjjpMa4c2PcxhWdJfN4FIwQ1DQxDFAiuD-Bbpf9DKHcgbeeDVm9_55n4K7r7vbi-_N1fW3y4vzq8ZQwUtjvO49a7nuLRFcIe-85YpZLohltvXeYk47yhFmmveK0Z5r2mrrsfZeUEVPwee195Di78XlIqeQjRtHNbu4ZNkJ3nOB-H9BzEktF10F0QqaFHNOzstDClN9jsRIPhqS1ZCshiSh8slQjXx87l705OyLwKqkAp9WYAi_hj8hOalDrH-c_u15APMVmco</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17238796</pqid></control><display><type>article</type><title>Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Noah, J W ; Dolan, M A ; Babin, P ; Wollenzien, P</creator><creatorcontrib>Noah, J W ; Dolan, M A ; Babin, P ; Wollenzien, P</creatorcontrib><description>Structural analysis of the 16 S rRNA in the 30 S subunit and 70 S ribosome in the presence of ribosome-specific antibiotics
was performed to determine whether they produced rRNA structural changes that might provide further insight to their action.
An UV cross-linking procedure that determines the pattern and frequency of intramolecular 16 S RNA cross-links was used to
detect differences reflecting structural changes. Tetracycline and spectinomycin have specific effects detected by this assay.
The presence of tetracycline inhibits the cross-link C967ÃC1400 completely, increases the frequency of cross-link C1402Ã1501
twofold, and decreases the cross-link G894ÃU244 by one-half without affecting other cross-links. Spectinomycin reduces the
frequency of the cross-link C934ÃU1345 by 60% without affecting cross-linking at other sites. The structural changes occur
at concentrations at which the antibiotics exert their inhibitory effects. For spectinomycin, the apparent binding site and
the affected cross-linking site are distant in the secondary structure but are close in tertiary structure in several recent
models, indicating a localized effect. For tetracycline, the apparent binding sites are significantly separated in both the
secondary and the three-dimensional structures, suggesting a more regional effect.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.274.23.16576</identifier><identifier>PMID: 10347223</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Anti-Bacterial Agents - pharmacology ; Base Sequence ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - radiation effects ; Models, Molecular ; Molecular Sequence Data ; Nucleic Acid Conformation ; Ribosomes - radiation effects ; Ribosomes - ultrastructure ; RNA, Bacterial - drug effects ; RNA, Bacterial - metabolism ; RNA, Bacterial - radiation effects ; RNA, Ribosomal, 16S - drug effects ; RNA, Ribosomal, 16S - radiation effects ; RNA, Ribosomal, 16S - ultrastructure ; Spectinomycin - pharmacology ; Tetracycline - pharmacology ; Ultraviolet Rays</subject><ispartof>The Journal of biological chemistry, 1999-06, Vol.274 (23), p.16576-16581</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-cfb8f457b8d297a0fefd7a4d792d4d5ffd173637014b78a4387b35bdf1bff93a3</citedby><cites>FETCH-LOGICAL-c397t-cfb8f457b8d297a0fefd7a4d792d4d5ffd173637014b78a4387b35bdf1bff93a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10347223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noah, J W</creatorcontrib><creatorcontrib>Dolan, M A</creatorcontrib><creatorcontrib>Babin, P</creatorcontrib><creatorcontrib>Wollenzien, P</creatorcontrib><title>Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Structural analysis of the 16 S rRNA in the 30 S subunit and 70 S ribosome in the presence of ribosome-specific antibiotics
was performed to determine whether they produced rRNA structural changes that might provide further insight to their action.
An UV cross-linking procedure that determines the pattern and frequency of intramolecular 16 S RNA cross-links was used to
detect differences reflecting structural changes. Tetracycline and spectinomycin have specific effects detected by this assay.
The presence of tetracycline inhibits the cross-link C967ÃC1400 completely, increases the frequency of cross-link C1402Ã1501
twofold, and decreases the cross-link G894ÃU244 by one-half without affecting other cross-links. Spectinomycin reduces the
frequency of the cross-link C934ÃU1345 by 60% without affecting cross-linking at other sites. The structural changes occur
at concentrations at which the antibiotics exert their inhibitory effects. For spectinomycin, the apparent binding site and
the affected cross-linking site are distant in the secondary structure but are close in tertiary structure in several recent
models, indicating a localized effect. For tetracycline, the apparent binding sites are significantly separated in both the
secondary and the three-dimensional structures, suggesting a more regional effect.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Base Sequence</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - radiation effects</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Ribosomes - radiation effects</subject><subject>Ribosomes - ultrastructure</subject><subject>RNA, Bacterial - drug effects</subject><subject>RNA, Bacterial - metabolism</subject><subject>RNA, Bacterial - radiation effects</subject><subject>RNA, Ribosomal, 16S - drug effects</subject><subject>RNA, Ribosomal, 16S - radiation effects</subject><subject>RNA, Ribosomal, 16S - ultrastructure</subject><subject>Spectinomycin - pharmacology</subject><subject>Tetracycline - pharmacology</subject><subject>Ultraviolet Rays</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEURi0EIktCT4VcILpZ_JrxuIyiBSJFRMomEp3lJ-NoZrzYHqGt-eNxMilCxW1ucc_3FfcA8AGjLUacfbnXZks42xK6xV3Lu1dgg1FPG9rin6_BBiGCG0Ha_gS8y_ke1WECvwUnGFHGCaEb8HfnvTMlw-jhrStJmaMZw-ygmi3cH-opzHE6mjDDOMMyuEqlElQ6wn1JiylLco_Zm6BjjpMa4c2PcxhWdJfN4FIwQ1DQxDFAiuD-Bbpf9DKHcgbeeDVm9_55n4K7r7vbi-_N1fW3y4vzq8ZQwUtjvO49a7nuLRFcIe-85YpZLohltvXeYk47yhFmmveK0Z5r2mrrsfZeUEVPwee195Di78XlIqeQjRtHNbu4ZNkJ3nOB-H9BzEktF10F0QqaFHNOzstDClN9jsRIPhqS1ZCshiSh8slQjXx87l705OyLwKqkAp9WYAi_hj8hOalDrH-c_u15APMVmco</recordid><startdate>19990604</startdate><enddate>19990604</enddate><creator>Noah, J W</creator><creator>Dolan, M A</creator><creator>Babin, P</creator><creator>Wollenzien, P</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19990604</creationdate><title>Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit</title><author>Noah, J W ; Dolan, M A ; Babin, P ; Wollenzien, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-cfb8f457b8d297a0fefd7a4d792d4d5ffd173637014b78a4387b35bdf1bff93a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Base Sequence</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - radiation effects</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Ribosomes - radiation effects</topic><topic>Ribosomes - ultrastructure</topic><topic>RNA, Bacterial - drug effects</topic><topic>RNA, Bacterial - metabolism</topic><topic>RNA, Bacterial - radiation effects</topic><topic>RNA, Ribosomal, 16S - drug effects</topic><topic>RNA, Ribosomal, 16S - radiation effects</topic><topic>RNA, Ribosomal, 16S - ultrastructure</topic><topic>Spectinomycin - pharmacology</topic><topic>Tetracycline - pharmacology</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noah, J W</creatorcontrib><creatorcontrib>Dolan, M A</creatorcontrib><creatorcontrib>Babin, P</creatorcontrib><creatorcontrib>Wollenzien, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noah, J W</au><au>Dolan, M A</au><au>Babin, P</au><au>Wollenzien, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1999-06-04</date><risdate>1999</risdate><volume>274</volume><issue>23</issue><spage>16576</spage><epage>16581</epage><pages>16576-16581</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Structural analysis of the 16 S rRNA in the 30 S subunit and 70 S ribosome in the presence of ribosome-specific antibiotics
was performed to determine whether they produced rRNA structural changes that might provide further insight to their action.
An UV cross-linking procedure that determines the pattern and frequency of intramolecular 16 S RNA cross-links was used to
detect differences reflecting structural changes. Tetracycline and spectinomycin have specific effects detected by this assay.
The presence of tetracycline inhibits the cross-link C967ÃC1400 completely, increases the frequency of cross-link C1402Ã1501
twofold, and decreases the cross-link G894ÃU244 by one-half without affecting other cross-links. Spectinomycin reduces the
frequency of the cross-link C934ÃU1345 by 60% without affecting cross-linking at other sites. The structural changes occur
at concentrations at which the antibiotics exert their inhibitory effects. For spectinomycin, the apparent binding site and
the affected cross-linking site are distant in the secondary structure but are close in tertiary structure in several recent
models, indicating a localized effect. For tetracycline, the apparent binding sites are significantly separated in both the
secondary and the three-dimensional structures, suggesting a more regional effect.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>10347223</pmid><doi>10.1074/jbc.274.23.16576</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1999-06, Vol.274 (23), p.16576-16581 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69787907 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Anti-Bacterial Agents - pharmacology Base Sequence Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - radiation effects Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Ribosomes - radiation effects Ribosomes - ultrastructure RNA, Bacterial - drug effects RNA, Bacterial - metabolism RNA, Bacterial - radiation effects RNA, Ribosomal, 16S - drug effects RNA, Ribosomal, 16S - radiation effects RNA, Ribosomal, 16S - ultrastructure Spectinomycin - pharmacology Tetracycline - pharmacology Ultraviolet Rays |
| title | Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T15%3A35%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20Tetracycline%20and%20Spectinomycin%20on%20the%20Tertiary%20Structure%20of%20Ribosomal%20RNA%20in%20the%20Escherichia%20coli%2030%20S%20Ribosomal%20Subunit&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Noah,%20J%20W&rft.date=1999-06-04&rft.volume=274&rft.issue=23&rft.spage=16576&rft.epage=16581&rft.pages=16576-16581&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.274.23.16576&rft_dat=%3Cproquest_cross%3E17238796%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17238796&rft_id=info:pmid/10347223&rfr_iscdi=true |