Isolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato
After differential screening we isolated cDNA clones encoding a histone H1 (leH1) and three variants of histone H2B (leH2B-1, -2 and -3) from the gibberellin (GA)-deficient mutant of tomato (gib-1). The deduced polypeptide of leH1 is 271 amino acids long and exhibits the typical tripartite structure...
Gespeichert in:
| Veröffentlicht in: | Plant molecular biology 1999-03, Vol.39 (5), p.883-890 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 890 |
|---|---|
| container_issue | 5 |
| container_start_page | 883 |
| container_title | Plant molecular biology |
| container_volume | 39 |
| creator | Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany) Esch, R.J. van Barendse, G.W.M Wullems, G.J |
| description | After differential screening we isolated cDNA clones encoding a histone H1 (leH1) and three variants of histone H2B (leH2B-1, -2 and -3) from the gibberellin (GA)-deficient mutant of tomato (gib-1). The deduced polypeptide of leH1 is 271 amino acids long and exhibits the typical tripartite structure of histones H1. The full-length cDNA clone leH2B-1 encodes for a protein of 142 amino residues and shows the tripartite organization of histones H2B. The histones leH1 and leH2B, which show no tissue specificity, are developmentally expressed in the leaf. The mRNA accumulation was higher in organs which contain meristematic tissue and/or which have a high proportion of actively cycling cells. In the leaf of the gib-1 mutant we demonstrated GA-enhanced histone leH1 and leH2B expression which was not observed in the wild type. GAs of the early-13-hydroxylated pathway (GA1 and GA3) caused most enhanced transcription compared to GAs of the early-non-hydroxylation pathway (GA4 and GA9). Application of GA to the mutant increased histone expression that could correlate with enhanced DNA replication in leaf tissue. Increased chromosome replication may indicate that there is a higher rate of cell division and/or increase of endopolyploidy which both may be dependent on cell elongation induced by GAs. |
| doi_str_mv | 10.1023/A:1006157718263 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69781595</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69781595</sourcerecordid><originalsourceid>FETCH-LOGICAL-c302t-f8a3405637b37d284c77a4b7a7963f32373e4202805a0486515816e36af5d6373</originalsourceid><addsrcrecordid>eNpdkU1P3DAQhq0KVBbouadWFgduof52wm35XKQVXNpzNEkmu0ZJTG3nQH8Cv7reLpWqnmY087yjV-8Q8pmzC86E_La85IwZrq3lpTDyA1nkXhaaifKALBg3tlCKiyNyHOMzYxmW5iM54kzmcaUW5O0h-gGS8xOFqaOjH7CdBwi03UKANmFwv_Zr39ONaxoMOAxuKgJuMpewoyv-R7oSV3TrYvIT0vbmcRmpm2jaIh0Q-p161_97ocPetQ6nRJMfIflTctjDEPHTez0hP-5uv1-vivXT_cP1cl20kolU9CVIxbSRtpG2E6VqrQXVWLCVkb0U0kpUIgfANDBVGs11yQ1KA73uskqekPP93Zfgf84YUz262GZPMKGfY20qW3Jd6Qye_Qc--zlM2Vud81ZaCb2Dvr5DczNiV78EN0J4rf9GnIEve6AHX8MmuFg_rnlVVfkXFTfyNw_PhRo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>771454255</pqid></control><display><type>article</type><title>Isolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany) ; Esch, R.J. van ; Barendse, G.W.M ; Wullems, G.J</creator><creatorcontrib>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany) ; Esch, R.J. van ; Barendse, G.W.M ; Wullems, G.J</creatorcontrib><description>After differential screening we isolated cDNA clones encoding a histone H1 (leH1) and three variants of histone H2B (leH2B-1, -2 and -3) from the gibberellin (GA)-deficient mutant of tomato (gib-1). The deduced polypeptide of leH1 is 271 amino acids long and exhibits the typical tripartite structure of histones H1. The full-length cDNA clone leH2B-1 encodes for a protein of 142 amino residues and shows the tripartite organization of histones H2B. The histones leH1 and leH2B, which show no tissue specificity, are developmentally expressed in the leaf. The mRNA accumulation was higher in organs which contain meristematic tissue and/or which have a high proportion of actively cycling cells. In the leaf of the gib-1 mutant we demonstrated GA-enhanced histone leH1 and leH2B expression which was not observed in the wild type. GAs of the early-13-hydroxylated pathway (GA1 and GA3) caused most enhanced transcription compared to GAs of the early-non-hydroxylation pathway (GA4 and GA9). Application of GA to the mutant increased histone expression that could correlate with enhanced DNA replication in leaf tissue. Increased chromosome replication may indicate that there is a higher rate of cell division and/or increase of endopolyploidy which both may be dependent on cell elongation induced by GAs.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1006157718263</identifier><identifier>PMID: 10344194</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; Amino acids ; Blotting, Northern ; Cell division ; Cloning, Molecular ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; DNA, Complementary - isolation & purification ; EXPRESION GENICA ; EXPRESSION DES GENES ; FEUILLE ; GENE EXPRESSION ; Gene Expression Regulation, Plant ; Gibberellins - metabolism ; Gibberellins - pharmacology ; HISTONAS ; HISTONE ; HISTONES ; Histones - genetics ; HOJAS ; LEAVES ; LYCOPERSICON ESCULENTUM ; Lycopersicon esculentum - chemistry ; Lycopersicon esculentum - drug effects ; Lycopersicon esculentum - genetics ; Molecular Sequence Data ; Mutation ; Plant Leaves - chemistry ; Plant Leaves - genetics ; Plant tissues ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Tissue Distribution ; Tomatoes</subject><ispartof>Plant molecular biology, 1999-03, Vol.39 (5), p.883-890</ispartof><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-f8a3405637b37d284c77a4b7a7963f32373e4202805a0486515816e36af5d6373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10344194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany)</creatorcontrib><creatorcontrib>Esch, R.J. van</creatorcontrib><creatorcontrib>Barendse, G.W.M</creatorcontrib><creatorcontrib>Wullems, G.J</creatorcontrib><title>Isolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>After differential screening we isolated cDNA clones encoding a histone H1 (leH1) and three variants of histone H2B (leH2B-1, -2 and -3) from the gibberellin (GA)-deficient mutant of tomato (gib-1). The deduced polypeptide of leH1 is 271 amino acids long and exhibits the typical tripartite structure of histones H1. The full-length cDNA clone leH2B-1 encodes for a protein of 142 amino residues and shows the tripartite organization of histones H2B. The histones leH1 and leH2B, which show no tissue specificity, are developmentally expressed in the leaf. The mRNA accumulation was higher in organs which contain meristematic tissue and/or which have a high proportion of actively cycling cells. In the leaf of the gib-1 mutant we demonstrated GA-enhanced histone leH1 and leH2B expression which was not observed in the wild type. GAs of the early-13-hydroxylated pathway (GA1 and GA3) caused most enhanced transcription compared to GAs of the early-non-hydroxylation pathway (GA4 and GA9). Application of GA to the mutant increased histone expression that could correlate with enhanced DNA replication in leaf tissue. Increased chromosome replication may indicate that there is a higher rate of cell division and/or increase of endopolyploidy which both may be dependent on cell elongation induced by GAs.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Blotting, Northern</subject><subject>Cell division</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FEUILLE</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gibberellins - metabolism</subject><subject>Gibberellins - pharmacology</subject><subject>HISTONAS</subject><subject>HISTONE</subject><subject>HISTONES</subject><subject>Histones - genetics</subject><subject>HOJAS</subject><subject>LEAVES</subject><subject>LYCOPERSICON ESCULENTUM</subject><subject>Lycopersicon esculentum - chemistry</subject><subject>Lycopersicon esculentum - drug effects</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - genetics</subject><subject>Plant tissues</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tissue Distribution</subject><subject>Tomatoes</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1P3DAQhq0KVBbouadWFgduof52wm35XKQVXNpzNEkmu0ZJTG3nQH8Cv7reLpWqnmY087yjV-8Q8pmzC86E_La85IwZrq3lpTDyA1nkXhaaifKALBg3tlCKiyNyHOMzYxmW5iM54kzmcaUW5O0h-gGS8xOFqaOjH7CdBwi03UKANmFwv_Zr39ONaxoMOAxuKgJuMpewoyv-R7oSV3TrYvIT0vbmcRmpm2jaIh0Q-p161_97ocPetQ6nRJMfIflTctjDEPHTez0hP-5uv1-vivXT_cP1cl20kolU9CVIxbSRtpG2E6VqrQXVWLCVkb0U0kpUIgfANDBVGs11yQ1KA73uskqekPP93Zfgf84YUz262GZPMKGfY20qW3Jd6Qye_Qc--zlM2Vud81ZaCb2Dvr5DczNiV78EN0J4rf9GnIEve6AHX8MmuFg_rnlVVfkXFTfyNw_PhRo</recordid><startdate>199903</startdate><enddate>199903</enddate><creator>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany)</creator><creator>Esch, R.J. van</creator><creator>Barendse, G.W.M</creator><creator>Wullems, G.J</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199903</creationdate><title>Isolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato</title><author>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany) ; Esch, R.J. van ; Barendse, G.W.M ; Wullems, G.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-f8a3405637b37d284c77a4b7a7963f32373e4202805a0486515816e36af5d6373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Blotting, Northern</topic><topic>Cell division</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FEUILLE</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gibberellins - metabolism</topic><topic>Gibberellins - pharmacology</topic><topic>HISTONAS</topic><topic>HISTONE</topic><topic>HISTONES</topic><topic>Histones - genetics</topic><topic>HOJAS</topic><topic>LEAVES</topic><topic>LYCOPERSICON ESCULENTUM</topic><topic>Lycopersicon esculentum - chemistry</topic><topic>Lycopersicon esculentum - drug effects</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - genetics</topic><topic>Plant tissues</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tissue Distribution</topic><topic>Tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany)</creatorcontrib><creatorcontrib>Esch, R.J. van</creatorcontrib><creatorcontrib>Barendse, G.W.M</creatorcontrib><creatorcontrib>Wullems, G.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heuvel, K.J.P.T. van den (Nijmegen Univ., (Netherlands). Dept. of Experimental Botany)</au><au>Esch, R.J. van</au><au>Barendse, G.W.M</au><au>Wullems, G.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1999-03</date><risdate>1999</risdate><volume>39</volume><issue>5</issue><spage>883</spage><epage>890</epage><pages>883-890</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>After differential screening we isolated cDNA clones encoding a histone H1 (leH1) and three variants of histone H2B (leH2B-1, -2 and -3) from the gibberellin (GA)-deficient mutant of tomato (gib-1). The deduced polypeptide of leH1 is 271 amino acids long and exhibits the typical tripartite structure of histones H1. The full-length cDNA clone leH2B-1 encodes for a protein of 142 amino residues and shows the tripartite organization of histones H2B. The histones leH1 and leH2B, which show no tissue specificity, are developmentally expressed in the leaf. The mRNA accumulation was higher in organs which contain meristematic tissue and/or which have a high proportion of actively cycling cells. In the leaf of the gib-1 mutant we demonstrated GA-enhanced histone leH1 and leH2B expression which was not observed in the wild type. GAs of the early-13-hydroxylated pathway (GA1 and GA3) caused most enhanced transcription compared to GAs of the early-non-hydroxylation pathway (GA4 and GA9). Application of GA to the mutant increased histone expression that could correlate with enhanced DNA replication in leaf tissue. Increased chromosome replication may indicate that there is a higher rate of cell division and/or increase of endopolyploidy which both may be dependent on cell elongation induced by GAs.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10344194</pmid><doi>10.1023/A:1006157718263</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1999-03, Vol.39 (5), p.883-890 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69781595 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acid Sequence Amino acids Blotting, Northern Cell division Cloning, Molecular DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Complementary - isolation & purification EXPRESION GENICA EXPRESSION DES GENES FEUILLE GENE EXPRESSION Gene Expression Regulation, Plant Gibberellins - metabolism Gibberellins - pharmacology HISTONAS HISTONE HISTONES Histones - genetics HOJAS LEAVES LYCOPERSICON ESCULENTUM Lycopersicon esculentum - chemistry Lycopersicon esculentum - drug effects Lycopersicon esculentum - genetics Molecular Sequence Data Mutation Plant Leaves - chemistry Plant Leaves - genetics Plant tissues RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution Tomatoes |
| title | Isolation and molecular characterization of gibberellin-regulated H1 and H2B histone cDNAs in the leaf of the gibberellin-deficient tomato |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T13%3A49%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20molecular%20characterization%20of%20gibberellin-regulated%20H1%20and%20H2B%20histone%20cDNAs%20in%20the%20leaf%20of%20the%20gibberellin-deficient%20tomato&rft.jtitle=Plant%20molecular%20biology&rft.au=Heuvel,%20K.J.P.T.%20van%20den%20(Nijmegen%20Univ.,%20(Netherlands).%20Dept.%20of%20Experimental%20Botany)&rft.date=1999-03&rft.volume=39&rft.issue=5&rft.spage=883&rft.epage=890&rft.pages=883-890&rft.issn=0167-4412&rft.eissn=1573-5028&rft_id=info:doi/10.1023/A:1006157718263&rft_dat=%3Cproquest_pubme%3E69781595%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=771454255&rft_id=info:pmid/10344194&rfr_iscdi=true |