Characterization of the antiseptic-resistance gene qacEΔ 1 isolated from clinical and environmental isolates of Vibrio parahaemolyticus and Vibrio cholerae non-O1
The nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene qacEΔ 1 that had been isolated from Pseudomonas aeruginosa, Vibrio parahaemolyticus and Vibrio cholerae non-O1. The nucleotide sequences of qacEΔ 1 genes isolated from environmental isolates of V. cholera...
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| Veröffentlicht in: | FEMS microbiology letters 1999-05, Vol.174 (2), p.379-384 |
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| creator | Kazama, Hitoshi Hamashima, Hajime Sasatsu, Masanori Arai, Taketoshi |
| description | The nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene
qacEΔ
1 that had been isolated from
Pseudomonas aeruginosa,
Vibrio parahaemolyticus and
Vibrio cholerae non-O1. The nucleotide sequences of
qacEΔ
1 genes isolated from environmental isolates of
V. cholerae non-O1 and
V. parahaemolyticus differed by one base from that of the gene from
P. aeruginosa.
Escherichia coli C600 that harbored
qacEΔ
1 genes from several strains of
Vibrio spp. exhibited low-level resistance to intercalating dyes. The resistance of
E. coli cells with these genes to intercalating dyes, such as ethidium bromide, was mediated by an efflux system. Moreover, the activity of QacEΔ1 was inhibited in the presence of calcium channel blockers but not of calmodulin inhibitors. These results indicate that the
qacEΔ
1 gene can be function in
E. coli and that the gene mediates resistance in a similar manner to the antiseptic-resistance gene
smr. |
| doi_str_mv | 10.1016/S0378-1097(99)00167-6 |
| format | Article |
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qacEΔ
1 that had been isolated from
Pseudomonas aeruginosa,
Vibrio parahaemolyticus and
Vibrio cholerae non-O1. The nucleotide sequences of
qacEΔ
1 genes isolated from environmental isolates of
V. cholerae non-O1 and
V. parahaemolyticus differed by one base from that of the gene from
P. aeruginosa.
Escherichia coli C600 that harbored
qacEΔ
1 genes from several strains of
Vibrio spp. exhibited low-level resistance to intercalating dyes. The resistance of
E. coli cells with these genes to intercalating dyes, such as ethidium bromide, was mediated by an efflux system. Moreover, the activity of QacEΔ1 was inhibited in the presence of calcium channel blockers but not of calmodulin inhibitors. These results indicate that the
qacEΔ
1 gene can be function in
E. coli and that the gene mediates resistance in a similar manner to the antiseptic-resistance gene
smr.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(99)00167-6</identifier><identifier>PMID: 10339831</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Anti-Infective Agents, Local - pharmacology ; Antiseptic resistance ; Cholera - microbiology ; Coloring Agents - pharmacology ; Drug Resistance, Microbial - genetics ; Efflux ; Environmental Microbiology ; Genes, Bacterial ; Humans ; Integron ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Plasmids - genetics ; Pseudomonas aeruginosa ; qac ; Sequence Analysis, DNA ; Vibrio ; Vibrio cholerae ; Vibrio cholerae - drug effects ; Vibrio cholerae - genetics ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - drug effects ; Vibrio parahaemolyticus - genetics</subject><ispartof>FEMS microbiology letters, 1999-05, Vol.174 (2), p.379-384</ispartof><rights>1999 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10339831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kazama, Hitoshi</creatorcontrib><creatorcontrib>Hamashima, Hajime</creatorcontrib><creatorcontrib>Sasatsu, Masanori</creatorcontrib><creatorcontrib>Arai, Taketoshi</creatorcontrib><title>Characterization of the antiseptic-resistance gene qacEΔ 1 isolated from clinical and environmental isolates of Vibrio parahaemolyticus and Vibrio cholerae non-O1</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>The nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene
qacEΔ
1 that had been isolated from
Pseudomonas aeruginosa,
Vibrio parahaemolyticus and
Vibrio cholerae non-O1. The nucleotide sequences of
qacEΔ
1 genes isolated from environmental isolates of
V. cholerae non-O1 and
V. parahaemolyticus differed by one base from that of the gene from
P. aeruginosa.
Escherichia coli C600 that harbored
qacEΔ
1 genes from several strains of
Vibrio spp. exhibited low-level resistance to intercalating dyes. The resistance of
E. coli cells with these genes to intercalating dyes, such as ethidium bromide, was mediated by an efflux system. Moreover, the activity of QacEΔ1 was inhibited in the presence of calcium channel blockers but not of calmodulin inhibitors. These results indicate that the
qacEΔ
1 gene can be function in
E. coli and that the gene mediates resistance in a similar manner to the antiseptic-resistance gene
smr.</description><subject>Amino Acid Sequence</subject><subject>Anti-Infective Agents, Local - pharmacology</subject><subject>Antiseptic resistance</subject><subject>Cholera - microbiology</subject><subject>Coloring Agents - pharmacology</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Efflux</subject><subject>Environmental Microbiology</subject><subject>Genes, Bacterial</subject><subject>Humans</subject><subject>Integron</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Plasmids - genetics</subject><subject>Pseudomonas aeruginosa</subject><subject>qac</subject><subject>Sequence Analysis, DNA</subject><subject>Vibrio</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - drug effects</subject><subject>Vibrio cholerae - genetics</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - drug effects</subject><subject>Vibrio parahaemolyticus - genetics</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd1qVDEQx4Modq0-gpIr0YujyUk2ObkSWeoHFHrhx22YTea4kXOSbZIt1OfwDXwfn6nZ7ba3hYGBmd_8h5k_IS85e8cZV--_MaGHjjOj3xjzlrWS7tQjsuBLLTtl1PCYLO6RE_KslN-MMdkz9ZSccCaEGQRfkH-rDWRwFXP4AzWkSNNI6wYpxBoKbmtwXcYSSoXokP7CiPQS3Nn_v5TTUNIEFT0dc5qpm0IMDqY26inGq5BTnDHWVjmCZS_-M6xzSHTb1m4A5zRdtx27cpg69twmTZgBaUyxu-DPyZMRpoIvjvmU_Ph09n31pTu_-Px19fG8Qy4570QvxTCYZa-4Y1xKKQyTvoWHXhjdAxi2HoXnyL12GpY4MJB8FKoHv2SDOCWvb3W3OV3usFQ7h-JwmiBi2hWrjB6acP8gyHWvtOhNA18dwd16Rm-3OcyQr-3d_xvw4RbAdtdVwGyLC9g-7UNGV61PocF277g9OG73dlpj7MFxq8QNCvCfMA</recordid><startdate>19990515</startdate><enddate>19990515</enddate><creator>Kazama, Hitoshi</creator><creator>Hamashima, Hajime</creator><creator>Sasatsu, Masanori</creator><creator>Arai, Taketoshi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990515</creationdate><title>Characterization of the antiseptic-resistance gene qacEΔ 1 isolated from clinical and environmental isolates of Vibrio parahaemolyticus and Vibrio cholerae non-O1</title><author>Kazama, Hitoshi ; Hamashima, Hajime ; Sasatsu, Masanori ; Arai, Taketoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1411-32438895261c014443904d04dda23972aa90bf3d1e1d7c7a5e80a41f362ad5083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Anti-Infective Agents, Local - pharmacology</topic><topic>Antiseptic resistance</topic><topic>Cholera - microbiology</topic><topic>Coloring Agents - pharmacology</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Efflux</topic><topic>Environmental Microbiology</topic><topic>Genes, Bacterial</topic><topic>Humans</topic><topic>Integron</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Plasmids - genetics</topic><topic>Pseudomonas aeruginosa</topic><topic>qac</topic><topic>Sequence Analysis, DNA</topic><topic>Vibrio</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - drug effects</topic><topic>Vibrio cholerae - genetics</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - drug effects</topic><topic>Vibrio parahaemolyticus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kazama, Hitoshi</creatorcontrib><creatorcontrib>Hamashima, Hajime</creatorcontrib><creatorcontrib>Sasatsu, Masanori</creatorcontrib><creatorcontrib>Arai, Taketoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kazama, Hitoshi</au><au>Hamashima, Hajime</au><au>Sasatsu, Masanori</au><au>Arai, Taketoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the antiseptic-resistance gene qacEΔ 1 isolated from clinical and environmental isolates of Vibrio parahaemolyticus and Vibrio cholerae non-O1</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1999-05-15</date><risdate>1999</risdate><volume>174</volume><issue>2</issue><spage>379</spage><epage>384</epage><pages>379-384</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>The nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene
qacEΔ
1 that had been isolated from
Pseudomonas aeruginosa,
Vibrio parahaemolyticus and
Vibrio cholerae non-O1. The nucleotide sequences of
qacEΔ
1 genes isolated from environmental isolates of
V. cholerae non-O1 and
V. parahaemolyticus differed by one base from that of the gene from
P. aeruginosa.
Escherichia coli C600 that harbored
qacEΔ
1 genes from several strains of
Vibrio spp. exhibited low-level resistance to intercalating dyes. The resistance of
E. coli cells with these genes to intercalating dyes, such as ethidium bromide, was mediated by an efflux system. Moreover, the activity of QacEΔ1 was inhibited in the presence of calcium channel blockers but not of calmodulin inhibitors. These results indicate that the
qacEΔ
1 gene can be function in
E. coli and that the gene mediates resistance in a similar manner to the antiseptic-resistance gene
smr.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10339831</pmid><doi>10.1016/S0378-1097(99)00167-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-05, Vol.174 (2), p.379-384 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69780142 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Anti-Infective Agents, Local - pharmacology Antiseptic resistance Cholera - microbiology Coloring Agents - pharmacology Drug Resistance, Microbial - genetics Efflux Environmental Microbiology Genes, Bacterial Humans Integron Microbial Sensitivity Tests Molecular Sequence Data Plasmids - genetics Pseudomonas aeruginosa qac Sequence Analysis, DNA Vibrio Vibrio cholerae Vibrio cholerae - drug effects Vibrio cholerae - genetics Vibrio parahaemolyticus Vibrio parahaemolyticus - drug effects Vibrio parahaemolyticus - genetics |
| title | Characterization of the antiseptic-resistance gene qacEΔ 1 isolated from clinical and environmental isolates of Vibrio parahaemolyticus and Vibrio cholerae non-O1 |
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