Establishment of a simple and practical procedure applicable to therapeutic angiogenesis
Therapeutic angiogenesis is thought to be beneficial for serious ischemic diseases. This investigation was designed to establish a simple and practical procedure applicable to therapeutic angiogenesis. When cultured skeletal muscle cells were electrically stimulated at a voltage that did not cause t...
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| Veröffentlicht in: | Circulation (New York, N.Y.) N.Y.), 1999-05, Vol.99 (20), p.2682-2687 |
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| container_title | Circulation (New York, N.Y.) |
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| creator | KANNO, S ODA, N ABE, M SAITO, S HORI, K HANDA, Y TABAYASHI, K SATO, Y |
| description | Therapeutic angiogenesis is thought to be beneficial for serious ischemic diseases. This investigation was designed to establish a simple and practical procedure applicable to therapeutic angiogenesis.
When cultured skeletal muscle cells were electrically stimulated at a voltage that did not cause their contraction, vascular endothelial growth factor (VEGF) mRNA was augmented at an optimal-frequency stimulation. This increase of VEGF mRNA was derived primarily from transcriptional activation. Electrical stimulation increased the secretion of VEGF protein into the medium. This conditioned medium then augmented the growth of endothelial cells. The effect of electrical stimulation was further confirmed in a rat model of hindlimb ischemia. The tibialis anterior muscle in the ischemic limb was electrically stimulated. The frequency of stimulation was 50 Hz and strength was 0.1 V, which was far below the threshold for muscle contraction. After a 5-day stimulation, there was a significant increase in blood flow within the muscle. Immunohistochemical analysis revealed that VEGF protein was synthesized and capillary density was significantly increased in the stimulated muscle. Rats tolerated this procedure very well, and there was no muscle contraction, muscle injury, or restriction in movement.
We propose this procedure as a simple and practical method of therapeutic angiogenesis. |
| doi_str_mv | 10.1161/01.cir.99.20.2682 |
| format | Article |
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When cultured skeletal muscle cells were electrically stimulated at a voltage that did not cause their contraction, vascular endothelial growth factor (VEGF) mRNA was augmented at an optimal-frequency stimulation. This increase of VEGF mRNA was derived primarily from transcriptional activation. Electrical stimulation increased the secretion of VEGF protein into the medium. This conditioned medium then augmented the growth of endothelial cells. The effect of electrical stimulation was further confirmed in a rat model of hindlimb ischemia. The tibialis anterior muscle in the ischemic limb was electrically stimulated. The frequency of stimulation was 50 Hz and strength was 0.1 V, which was far below the threshold for muscle contraction. After a 5-day stimulation, there was a significant increase in blood flow within the muscle. Immunohistochemical analysis revealed that VEGF protein was synthesized and capillary density was significantly increased in the stimulated muscle. Rats tolerated this procedure very well, and there was no muscle contraction, muscle injury, or restriction in movement.
We propose this procedure as a simple and practical method of therapeutic angiogenesis.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/01.cir.99.20.2682</identifier><identifier>PMID: 10338463</identifier><identifier>CODEN: CIRCAZ</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Animals ; Aorta - cytology ; Aorta - metabolism ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology - methods ; Cardiology. Vascular system ; Cell Line ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Electric Stimulation ; Endothelial Growth Factors - biosynthesis ; Endothelial Growth Factors - genetics ; Endothelial Growth Factors - metabolism ; Hindlimb - blood supply ; Ischemia - therapy ; Lymphokines - biosynthesis ; Lymphokines - genetics ; Lymphokines - metabolism ; Male ; Medical sciences ; Mice ; Muscle, Skeletal - cytology ; Muscle, Skeletal - metabolism ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - metabolism ; Neovascularization, Physiologic - physiology ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - metabolism ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>Circulation (New York, N.Y.), 1999-05, Vol.99 (20), p.2682-2687</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. May 25, 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-fe7825a86d0ff6268a440d5550f4a7f1d9fc2a3e1e533160d3149dfe56b9fbfb3</citedby><cites>FETCH-LOGICAL-c504t-fe7825a86d0ff6268a440d5550f4a7f1d9fc2a3e1e533160d3149dfe56b9fbfb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3687,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1792161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10338463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KANNO, S</creatorcontrib><creatorcontrib>ODA, N</creatorcontrib><creatorcontrib>ABE, M</creatorcontrib><creatorcontrib>SAITO, S</creatorcontrib><creatorcontrib>HORI, K</creatorcontrib><creatorcontrib>HANDA, Y</creatorcontrib><creatorcontrib>TABAYASHI, K</creatorcontrib><creatorcontrib>SATO, Y</creatorcontrib><title>Establishment of a simple and practical procedure applicable to therapeutic angiogenesis</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>Therapeutic angiogenesis is thought to be beneficial for serious ischemic diseases. This investigation was designed to establish a simple and practical procedure applicable to therapeutic angiogenesis.
When cultured skeletal muscle cells were electrically stimulated at a voltage that did not cause their contraction, vascular endothelial growth factor (VEGF) mRNA was augmented at an optimal-frequency stimulation. This increase of VEGF mRNA was derived primarily from transcriptional activation. Electrical stimulation increased the secretion of VEGF protein into the medium. This conditioned medium then augmented the growth of endothelial cells. The effect of electrical stimulation was further confirmed in a rat model of hindlimb ischemia. The tibialis anterior muscle in the ischemic limb was electrically stimulated. The frequency of stimulation was 50 Hz and strength was 0.1 V, which was far below the threshold for muscle contraction. After a 5-day stimulation, there was a significant increase in blood flow within the muscle. Immunohistochemical analysis revealed that VEGF protein was synthesized and capillary density was significantly increased in the stimulated muscle. Rats tolerated this procedure very well, and there was no muscle contraction, muscle injury, or restriction in movement.
We propose this procedure as a simple and practical method of therapeutic angiogenesis.</description><subject>Animals</subject><subject>Aorta - cytology</subject><subject>Aorta - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology - methods</subject><subject>Cardiology. Vascular system</subject><subject>Cell Line</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Electric Stimulation</subject><subject>Endothelial Growth Factors - biosynthesis</subject><subject>Endothelial Growth Factors - genetics</subject><subject>Endothelial Growth Factors - metabolism</subject><subject>Hindlimb - blood supply</subject><subject>Ischemia - therapy</subject><subject>Lymphokines - biosynthesis</subject><subject>Lymphokines - genetics</subject><subject>Lymphokines - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - metabolism</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEtr3TAQhUVJaW7S_oBuigklO7ujt7UslzS5EAiUFroTsjxKFPyqZC_676twL7RkNQ--c5g5hHyk0FCq6BegjY-pMaZh0DDVsjdkRyUTtZDcnJEdAJhac8bOyUXOz2VUXMt35JwC561QfEd-3eTVdUPMTyNOazWHylU5jsuAlZv6aknOr9G7oXSzx35LZb8sQ1l1BVnnan3C5BbcClUUj3F-xAlzzO_J2-CGjB9O9ZL8_HbzY39X3z_cHvZf72svQax1QN0y6VrVQwiq_OCEgF5KCUE4HWhvgmeOI0XJOVXQcypMH1CqzoQudPySXB99y4G_N8yrHWP2OAxuwnnLVhmtDWhdwKtX4PO8pancZhllWoCAtkD0CPk055ww2CXF0aU_loJ9ydwCtfvDd2uMZWBfMi-aTyfjrRux_09xDLkAn0-AyyXKkNzkY_7HacOKM_8Lj8qKWQ</recordid><startdate>19990525</startdate><enddate>19990525</enddate><creator>KANNO, S</creator><creator>ODA, N</creator><creator>ABE, M</creator><creator>SAITO, S</creator><creator>HORI, K</creator><creator>HANDA, Y</creator><creator>TABAYASHI, K</creator><creator>SATO, Y</creator><general>Lippincott Williams & Wilkins</general><general>American Heart Association, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>19990525</creationdate><title>Establishment of a simple and practical procedure applicable to therapeutic angiogenesis</title><author>KANNO, S ; ODA, N ; ABE, M ; SAITO, S ; HORI, K ; HANDA, Y ; TABAYASHI, K ; SATO, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-fe7825a86d0ff6268a440d5550f4a7f1d9fc2a3e1e533160d3149dfe56b9fbfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Aorta - cytology</topic><topic>Aorta - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology - methods</topic><topic>Cardiology. Vascular system</topic><topic>Cell Line</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Electric Stimulation</topic><topic>Endothelial Growth Factors - biosynthesis</topic><topic>Endothelial Growth Factors - genetics</topic><topic>Endothelial Growth Factors - metabolism</topic><topic>Hindlimb - blood supply</topic><topic>Ischemia - therapy</topic><topic>Lymphokines - biosynthesis</topic><topic>Lymphokines - genetics</topic><topic>Lymphokines - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - metabolism</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KANNO, S</creatorcontrib><creatorcontrib>ODA, N</creatorcontrib><creatorcontrib>ABE, M</creatorcontrib><creatorcontrib>SAITO, S</creatorcontrib><creatorcontrib>HORI, K</creatorcontrib><creatorcontrib>HANDA, Y</creatorcontrib><creatorcontrib>TABAYASHI, K</creatorcontrib><creatorcontrib>SATO, Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KANNO, S</au><au>ODA, N</au><au>ABE, M</au><au>SAITO, S</au><au>HORI, K</au><au>HANDA, Y</au><au>TABAYASHI, K</au><au>SATO, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a simple and practical procedure applicable to therapeutic angiogenesis</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>1999-05-25</date><risdate>1999</risdate><volume>99</volume><issue>20</issue><spage>2682</spage><epage>2687</epage><pages>2682-2687</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><coden>CIRCAZ</coden><abstract>Therapeutic angiogenesis is thought to be beneficial for serious ischemic diseases. This investigation was designed to establish a simple and practical procedure applicable to therapeutic angiogenesis.
When cultured skeletal muscle cells were electrically stimulated at a voltage that did not cause their contraction, vascular endothelial growth factor (VEGF) mRNA was augmented at an optimal-frequency stimulation. This increase of VEGF mRNA was derived primarily from transcriptional activation. Electrical stimulation increased the secretion of VEGF protein into the medium. This conditioned medium then augmented the growth of endothelial cells. The effect of electrical stimulation was further confirmed in a rat model of hindlimb ischemia. The tibialis anterior muscle in the ischemic limb was electrically stimulated. The frequency of stimulation was 50 Hz and strength was 0.1 V, which was far below the threshold for muscle contraction. After a 5-day stimulation, there was a significant increase in blood flow within the muscle. Immunohistochemical analysis revealed that VEGF protein was synthesized and capillary density was significantly increased in the stimulated muscle. Rats tolerated this procedure very well, and there was no muscle contraction, muscle injury, or restriction in movement.
We propose this procedure as a simple and practical method of therapeutic angiogenesis.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>10338463</pmid><doi>10.1161/01.cir.99.20.2682</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Circulation (New York, N.Y.), 1999-05, Vol.99 (20), p.2682-2687 |
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| source | MEDLINE; American Heart Association Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Complete |
| subjects | Animals Aorta - cytology Aorta - metabolism Biological and medical sciences Blood and lymphatic vessels Cardiology - methods Cardiology. Vascular system Cell Line Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Electric Stimulation Endothelial Growth Factors - biosynthesis Endothelial Growth Factors - genetics Endothelial Growth Factors - metabolism Hindlimb - blood supply Ischemia - therapy Lymphokines - biosynthesis Lymphokines - genetics Lymphokines - metabolism Male Medical sciences Mice Muscle, Skeletal - cytology Muscle, Skeletal - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - metabolism Neovascularization, Physiologic - physiology Rats Rats, Sprague-Dawley RNA, Messenger - metabolism Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | Establishment of a simple and practical procedure applicable to therapeutic angiogenesis |
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