Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance
A specific strain of Chlorella protothecoides has been studied in heterotrophic fermentation for increasing cell growth rate and lipid content for biodiesel production. For optimizing the process of fermentation to reduce costs of alga-based biodiesel production, rapid determination of lipid content...
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| Veröffentlicht in: | Journal of microbiological methods 2008-12, Vol.75 (3), p.437-440 |
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| container_title | Journal of microbiological methods |
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| creator | Gao, Chunfang Xiong, Wei Zhang, Yiliang Yuan, Wenqiao Wu, Qingyu |
| description | A specific strain of
Chlorella protothecoides has been studied in heterotrophic fermentation for increasing cell growth rate and lipid content for biodiesel production. For optimizing the process of fermentation to reduce costs of alga-based biodiesel production, rapid determination of lipid content in microalgal cells is critical. Nile Red (NR) staining and time-domain nuclear magnetic resonance (TD-NMR) have been investigated to quantitate the lipid content in
C. protothecoides. Both methods were found feasible and simpler than gravimetric methods that are commonly employed. The TD-NMR method showed better agreement (
R
2
=
0.9973) with the measured values from lipid extraction experiments than the NR staining method (
R
2
=
0.9067). Additionally, the smaller standard deviations of the samples (≤
0.36) analyzed by TD-NMR revealed that the method is accurate and reproducible. The application of TD-NMR for lipid quantitation in
C. protothecoides opens up the possibility of determining lipid content in algal fermentation precisely and quickly. |
| doi_str_mv | 10.1016/j.mimet.2008.07.019 |
| format | Article |
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Chlorella protothecoides has been studied in heterotrophic fermentation for increasing cell growth rate and lipid content for biodiesel production. For optimizing the process of fermentation to reduce costs of alga-based biodiesel production, rapid determination of lipid content in microalgal cells is critical. Nile Red (NR) staining and time-domain nuclear magnetic resonance (TD-NMR) have been investigated to quantitate the lipid content in
C. protothecoides. Both methods were found feasible and simpler than gravimetric methods that are commonly employed. The TD-NMR method showed better agreement (
R
2
=
0.9973) with the measured values from lipid extraction experiments than the NR staining method (
R
2
=
0.9067). Additionally, the smaller standard deviations of the samples (≤
0.36) analyzed by TD-NMR revealed that the method is accurate and reproducible. The application of TD-NMR for lipid quantitation in
C. protothecoides opens up the possibility of determining lipid content in algal fermentation precisely and quickly.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2008.07.019</identifier><identifier>PMID: 18706459</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biodiesel ; Biological and medical sciences ; Carbohydrates ; Chlorella - chemistry ; Chlorella - metabolism ; Chlorella protothecoides ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Lipid ; Lipids - chemistry ; Magnetic Resonance Spectroscopy - methods ; Microalgae ; Miscellaneous ; Nile Red ; Other biological molecules ; Oxazines - chemistry ; Staining and Labeling - methods ; Time-domain NMR</subject><ispartof>Journal of microbiological methods, 2008-12, Vol.75 (3), p.437-440</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-36f4bf0ce172db851950691a865159b186e91ef490b216a36d39a6937e1372c83</citedby><cites>FETCH-LOGICAL-c418t-36f4bf0ce172db851950691a865159b186e91ef490b216a36d39a6937e1372c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2008.07.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20897010$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18706459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Chunfang</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Zhang, Yiliang</creatorcontrib><creatorcontrib>Yuan, Wenqiao</creatorcontrib><creatorcontrib>Wu, Qingyu</creatorcontrib><title>Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>A specific strain of
Chlorella protothecoides has been studied in heterotrophic fermentation for increasing cell growth rate and lipid content for biodiesel production. For optimizing the process of fermentation to reduce costs of alga-based biodiesel production, rapid determination of lipid content in microalgal cells is critical. Nile Red (NR) staining and time-domain nuclear magnetic resonance (TD-NMR) have been investigated to quantitate the lipid content in
C. protothecoides. Both methods were found feasible and simpler than gravimetric methods that are commonly employed. The TD-NMR method showed better agreement (
R
2
=
0.9973) with the measured values from lipid extraction experiments than the NR staining method (
R
2
=
0.9067). Additionally, the smaller standard deviations of the samples (≤
0.36) analyzed by TD-NMR revealed that the method is accurate and reproducible. The application of TD-NMR for lipid quantitation in
C. protothecoides opens up the possibility of determining lipid content in algal fermentation precisely and quickly.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biodiesel</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Chlorella - chemistry</subject><subject>Chlorella - metabolism</subject><subject>Chlorella protothecoides</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipid</subject><subject>Lipids - chemistry</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Microalgae</subject><subject>Miscellaneous</subject><subject>Nile Red</subject><subject>Other biological molecules</subject><subject>Oxazines - chemistry</subject><subject>Staining and Labeling - methods</subject><subject>Time-domain NMR</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVoaTZpf0Gg-NLe7M5Ytj4OPZTQfECg0CZnIcvjoMUfG0kO5N9X213aW3MSSM-8M_OIsQuECgHFl201-YlSVQOoCmQFqE_YBpWsS8Vb_YZtMiVLCVifsrMYtwDY8ka9Y6cZAtG0esN-_bQ73xdPq52TTzb5ZS6WoRj9_tbPxeRdWOz4aKnoXoqUG5b9Mtn8Mq9uJBuKyT7OlLwrAsVltrOj9-ztYMdIH47nOXu4-n5_eVPe_bi-vfx2V7oGVSq5GJpuAEco675TLeoWhEarRIut7lAJ0khDo6GrUVgueq6t0FwSclk7xc_Z50PuLixPK8VkJh8djaOdaVmjEVrKLIK_CqJutMrtM8gPYF46xkCD2QU_2fBiEMxeutmaP9LNXroBabL0XPXxGL92E_X_ao6WM_DpCNjo7DiEbMnHv1wNSudfgsx9PXCUrT17CiY6T9lo7wO5ZPrF_3eQ36IpoCA</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Gao, Chunfang</creator><creator>Xiong, Wei</creator><creator>Zhang, Yiliang</creator><creator>Yuan, Wenqiao</creator><creator>Wu, Qingyu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance</title><author>Gao, Chunfang ; Xiong, Wei ; Zhang, Yiliang ; Yuan, Wenqiao ; Wu, Qingyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-36f4bf0ce172db851950691a865159b186e91ef490b216a36d39a6937e1372c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biodiesel</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Chlorella - chemistry</topic><topic>Chlorella - metabolism</topic><topic>Chlorella protothecoides</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipid</topic><topic>Lipids - chemistry</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Microalgae</topic><topic>Miscellaneous</topic><topic>Nile Red</topic><topic>Other biological molecules</topic><topic>Oxazines - chemistry</topic><topic>Staining and Labeling - methods</topic><topic>Time-domain NMR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Chunfang</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Zhang, Yiliang</creatorcontrib><creatorcontrib>Yuan, Wenqiao</creatorcontrib><creatorcontrib>Wu, Qingyu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Chunfang</au><au>Xiong, Wei</au><au>Zhang, Yiliang</au><au>Yuan, Wenqiao</au><au>Wu, Qingyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>75</volume><issue>3</issue><spage>437</spage><epage>440</epage><pages>437-440</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>A specific strain of
Chlorella protothecoides has been studied in heterotrophic fermentation for increasing cell growth rate and lipid content for biodiesel production. For optimizing the process of fermentation to reduce costs of alga-based biodiesel production, rapid determination of lipid content in microalgal cells is critical. Nile Red (NR) staining and time-domain nuclear magnetic resonance (TD-NMR) have been investigated to quantitate the lipid content in
C. protothecoides. Both methods were found feasible and simpler than gravimetric methods that are commonly employed. The TD-NMR method showed better agreement (
R
2
=
0.9973) with the measured values from lipid extraction experiments than the NR staining method (
R
2
=
0.9067). Additionally, the smaller standard deviations of the samples (≤
0.36) analyzed by TD-NMR revealed that the method is accurate and reproducible. The application of TD-NMR for lipid quantitation in
C. protothecoides opens up the possibility of determining lipid content in algal fermentation precisely and quickly.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18706459</pmid><doi>10.1016/j.mimet.2008.07.019</doi><tpages>4</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Biodiesel Biological and medical sciences Carbohydrates Chlorella - chemistry Chlorella - metabolism Chlorella protothecoides Fermentation Fundamental and applied biological sciences. Psychology Lipid Lipids - chemistry Magnetic Resonance Spectroscopy - methods Microalgae Miscellaneous Nile Red Other biological molecules Oxazines - chemistry Staining and Labeling - methods Time-domain NMR |
| title | Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance |
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