Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China
Abstract One hundred and forty-two Enterobacteriaceae isolates randomly selected from four general hospitals in Nanjing region of China were investigated for antibiotic susceptibility, the presence of integrons and the characterisation of gene cassettes. Eighty-five (59.9%) Enterobacteriaceae strain...
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| Veröffentlicht in: | International journal of antimicrobial agents 2008-12, Vol.32 (6), p.529-533 |
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| creator | Gu, Bing Pan, Shiyang Wang, Tong Zhao, Wenjun Mei, Yaning Huang, Peijun Tong, Mingqing |
| description | Abstract One hundred and forty-two Enterobacteriaceae isolates randomly selected from four general hospitals in Nanjing region of China were investigated for antibiotic susceptibility, the presence of integrons and the characterisation of gene cassettes. Eighty-five (59.9%) Enterobacteriaceae strains contained class 1 integrons, and 17 (65.4%) of 26 Shigella contained class 2 integrons. Class 3 integrons were not detected in this study. Restriction fragment length polymorphism (RFLP) and DNA sequencing analysis revealed seven different cassette arrays of class 1 integrons; additionally, four cassette arrays identified in this study were reported for the first time in some species. The genes most commonly found in these class 1 integrons were those for aminoglycoside and trimethoprim resistance. In conclusion, class 1 and 2 integrons were widespread in Enterobacteriaceae and Shigella , respectively, in Nanjing area of China and it was likely that integrons played an important role in antibiotic resistance. Characterisation of cassette arrays of integrons could be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes. |
| doi_str_mv | 10.1016/j.ijantimicag.2008.06.019 |
| format | Article |
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Eighty-five (59.9%) Enterobacteriaceae strains contained class 1 integrons, and 17 (65.4%) of 26 Shigella contained class 2 integrons. Class 3 integrons were not detected in this study. Restriction fragment length polymorphism (RFLP) and DNA sequencing analysis revealed seven different cassette arrays of class 1 integrons; additionally, four cassette arrays identified in this study were reported for the first time in some species. The genes most commonly found in these class 1 integrons were those for aminoglycoside and trimethoprim resistance. In conclusion, class 1 and 2 integrons were widespread in Enterobacteriaceae and Shigella , respectively, in Nanjing area of China and it was likely that integrons played an important role in antibiotic resistance. Characterisation of cassette arrays of integrons could be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2008.06.019</identifier><identifier>PMID: 18778917</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cassette array ; China ; Class 1 integron ; Class 2 integron ; Cross Infection - microbiology ; DNA, Bacterial - genetics ; Drug Resistance, Bacterial ; Enterobacteriaceae ; Enterobacteriaceae - genetics ; Enterobacteriaceae Infections - microbiology ; Genes, Bacterial - genetics ; Infectious Disease ; Integrons - genetics ; Microbial Sensitivity Tests ; Polymorphism, Restriction Fragment Length ; Resistance ; Reverse Transcriptase Polymerase Chain Reaction ; Shigella</subject><ispartof>International journal of antimicrobial agents, 2008-12, Vol.32 (6), p.529-533</ispartof><rights>Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2008 Elsevier B.V. and the International Society of Chemotherapy</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-83e1a9365100665fb2d1ca60be906bd74176ed4e3508d25f8bfda5bb7e21a4ad3</citedby><cites>FETCH-LOGICAL-c461t-83e1a9365100665fb2d1ca60be906bd74176ed4e3508d25f8bfda5bb7e21a4ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijantimicag.2008.06.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18778917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Bing</creatorcontrib><creatorcontrib>Pan, Shiyang</creatorcontrib><creatorcontrib>Wang, Tong</creatorcontrib><creatorcontrib>Zhao, Wenjun</creatorcontrib><creatorcontrib>Mei, Yaning</creatorcontrib><creatorcontrib>Huang, Peijun</creatorcontrib><creatorcontrib>Tong, Mingqing</creatorcontrib><title>Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>Abstract One hundred and forty-two Enterobacteriaceae isolates randomly selected from four general hospitals in Nanjing region of China were investigated for antibiotic susceptibility, the presence of integrons and the characterisation of gene cassettes. Eighty-five (59.9%) Enterobacteriaceae strains contained class 1 integrons, and 17 (65.4%) of 26 Shigella contained class 2 integrons. Class 3 integrons were not detected in this study. Restriction fragment length polymorphism (RFLP) and DNA sequencing analysis revealed seven different cassette arrays of class 1 integrons; additionally, four cassette arrays identified in this study were reported for the first time in some species. The genes most commonly found in these class 1 integrons were those for aminoglycoside and trimethoprim resistance. In conclusion, class 1 and 2 integrons were widespread in Enterobacteriaceae and Shigella , respectively, in Nanjing area of China and it was likely that integrons played an important role in antibiotic resistance. Characterisation of cassette arrays of integrons could be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.</description><subject>Cassette array</subject><subject>China</subject><subject>Class 1 integron</subject><subject>Class 2 integron</subject><subject>Cross Infection - microbiology</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Bacterial</subject><subject>Enterobacteriaceae</subject><subject>Enterobacteriaceae - genetics</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Genes, Bacterial - genetics</subject><subject>Infectious Disease</subject><subject>Integrons - genetics</subject><subject>Microbial Sensitivity Tests</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Resistance</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Shigella</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhi0EokvLX0Dhwi1hJo7t-IKEVuVDquiB9mw59qQ4ZJNiZyvtv8dhF4G40NPM4Zl5pWeGsdcIFQLKt0MVBjstYRecvatqgLYCWQHqJ2yDrapLpZE_ZRvQdVO2Qukz9iKlAQAFb8RzdpYh1WpUG3b7ZX6gsXA2JVoWKmyM9pCKuS_CtNBdnKeUu8KNYcphY5GWaMP0C7jMQJw763IJ1pGlldx-C5O9YM96OyZ6earn7PbD5c32U3l1_fHz9v1V6RqJS9lyQqu5FAggpei72qOzEjrSIDuvGlSSfENcQOtr0bdd763oOkU12sZ6fs7eHPfex_nHntJidiE5Gkc70bxPRmqlOEr-XxA1b0BykUF9BF2cU4rUm_sYdjYeDIJZ5ZvB_CXfrPINSJPl59lXp5B9tyP_Z_JkOwPbI0DZyUOgaJILNDnyIZJbjJ_Do2Le_bPl93W-04HSMO_jlKUbNKk2YL6uX7A-AbQAtRbIfwIhbbFj</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Gu, Bing</creator><creator>Pan, Shiyang</creator><creator>Wang, Tong</creator><creator>Zhao, Wenjun</creator><creator>Mei, Yaning</creator><creator>Huang, Peijun</creator><creator>Tong, Mingqing</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China</title><author>Gu, Bing ; Pan, Shiyang ; Wang, Tong ; Zhao, Wenjun ; Mei, Yaning ; Huang, Peijun ; Tong, Mingqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-83e1a9365100665fb2d1ca60be906bd74176ed4e3508d25f8bfda5bb7e21a4ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cassette array</topic><topic>China</topic><topic>Class 1 integron</topic><topic>Class 2 integron</topic><topic>Cross Infection - microbiology</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Bacterial</topic><topic>Enterobacteriaceae</topic><topic>Enterobacteriaceae - genetics</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Genes, Bacterial - genetics</topic><topic>Infectious Disease</topic><topic>Integrons - genetics</topic><topic>Microbial Sensitivity Tests</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Resistance</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Shigella</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Bing</creatorcontrib><creatorcontrib>Pan, Shiyang</creatorcontrib><creatorcontrib>Wang, Tong</creatorcontrib><creatorcontrib>Zhao, Wenjun</creatorcontrib><creatorcontrib>Mei, Yaning</creatorcontrib><creatorcontrib>Huang, Peijun</creatorcontrib><creatorcontrib>Tong, Mingqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Bing</au><au>Pan, Shiyang</au><au>Wang, Tong</au><au>Zhao, Wenjun</au><au>Mei, Yaning</au><au>Huang, Peijun</au><au>Tong, Mingqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>32</volume><issue>6</issue><spage>529</spage><epage>533</epage><pages>529-533</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>Abstract One hundred and forty-two Enterobacteriaceae isolates randomly selected from four general hospitals in Nanjing region of China were investigated for antibiotic susceptibility, the presence of integrons and the characterisation of gene cassettes. Eighty-five (59.9%) Enterobacteriaceae strains contained class 1 integrons, and 17 (65.4%) of 26 Shigella contained class 2 integrons. Class 3 integrons were not detected in this study. Restriction fragment length polymorphism (RFLP) and DNA sequencing analysis revealed seven different cassette arrays of class 1 integrons; additionally, four cassette arrays identified in this study were reported for the first time in some species. The genes most commonly found in these class 1 integrons were those for aminoglycoside and trimethoprim resistance. In conclusion, class 1 and 2 integrons were widespread in Enterobacteriaceae and Shigella , respectively, in Nanjing area of China and it was likely that integrons played an important role in antibiotic resistance. Characterisation of cassette arrays of integrons could be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18778917</pmid><doi>10.1016/j.ijantimicag.2008.06.019</doi><tpages>5</tpages></addata></record> |
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| subjects | Cassette array China Class 1 integron Class 2 integron Cross Infection - microbiology DNA, Bacterial - genetics Drug Resistance, Bacterial Enterobacteriaceae Enterobacteriaceae - genetics Enterobacteriaceae Infections - microbiology Genes, Bacterial - genetics Infectious Disease Integrons - genetics Microbial Sensitivity Tests Polymorphism, Restriction Fragment Length Resistance Reverse Transcriptase Polymerase Chain Reaction Shigella |
| title | Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China |
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