Functional Differentiation Signals Mediated by Distinct Regions of the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor Receptor
Granulocyte colony-stimulating factor receptor (G-CSFR) regulates the proliferation and differentiation of neutrophilic progenitor cells through interaction with its cytokine. Exposure of WEHI-3B D+ myelomonocytic leukemia and myeloid LGM-1 cells overexpressing the G-CSFR to G-CSF resulted in induct...
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Veröffentlicht in: | Blood 1999-06, Vol.93 (11), p.3774-3784 |
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description | Granulocyte colony-stimulating factor receptor (G-CSFR) regulates the proliferation and differentiation of neutrophilic progenitor cells through interaction with its cytokine. Exposure of WEHI-3B D+ myelomonocytic leukemia and myeloid LGM-1 cells overexpressing the G-CSFR to G-CSF resulted in induction of differentiation as measured by (1) the ability to reduce nitroblue tetrazolium (NBT), (2) the expression of Mac-I antigen, and (3) the expression of FcγII/III receptor. Mutational analyses indicated that distinct regions of the cytoplasmic domain were critical for efficient induction of each functional marker. The membrane proximal region containing homology sequences of boxes 1 and 2 was important for the activation of all three functional markers of mature neutrophils. Induction of the capacities to express Mac-I antigen or FcγII/III receptor also required additional sequences in the membrane proximal region between amino acids 70 and 100 and may be dependent on the phosphorylation of Tyr703. The findings suggest that distinct sequences within the amino-terminal region of the cytoplasmic domain of the receptor are sufficient to induce these functional markers of differentiation, and receptor tyrosine phosphorylation may be necessary. |
doi_str_mv | 10.1182/blood.V93.11.3774 |
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Exposure of WEHI-3B D+ myelomonocytic leukemia and myeloid LGM-1 cells overexpressing the G-CSFR to G-CSF resulted in induction of differentiation as measured by (1) the ability to reduce nitroblue tetrazolium (NBT), (2) the expression of Mac-I antigen, and (3) the expression of FcγII/III receptor. Mutational analyses indicated that distinct regions of the cytoplasmic domain were critical for efficient induction of each functional marker. The membrane proximal region containing homology sequences of boxes 1 and 2 was important for the activation of all three functional markers of mature neutrophils. Induction of the capacities to express Mac-I antigen or FcγII/III receptor also required additional sequences in the membrane proximal region between amino acids 70 and 100 and may be dependent on the phosphorylation of Tyr703. The findings suggest that distinct sequences within the amino-terminal region of the cytoplasmic domain of the receptor are sufficient to induce these functional markers of differentiation, and receptor tyrosine phosphorylation may be necessary.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V93.11.3774</identifier><identifier>PMID: 10339483</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell Differentiation ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; DNA Mutational Analysis ; Fundamental and applied biological sciences. Psychology ; Granulocyte Colony-Stimulating Factor - metabolism ; Granulocyte Colony-Stimulating Factor - pharmacology ; Macrophage-1 Antigen - biosynthesis ; Mice ; Molecular and cellular biology ; Receptors, Granulocyte Colony-Stimulating Factor - genetics ; Receptors, Granulocyte Colony-Stimulating Factor - metabolism ; Receptors, IgG - biosynthesis ; Signal Transduction - drug effects ; Tumor Cells, Cultured</subject><ispartof>Blood, 1999-06, Vol.93 (11), p.3774-3784</ispartof><rights>1999 American Society of Hematology</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2253-3516967fda93704c8e15d100e17d84e0b6d747d187d00c5e06534dd902c9446d3</citedby><cites>FETCH-LOGICAL-c2253-3516967fda93704c8e15d100e17d84e0b6d747d187d00c5e06534dd902c9446d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1831057$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10339483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koay, Debbie C.</creatorcontrib><creatorcontrib>Sartorelli, Alan C.</creatorcontrib><title>Functional Differentiation Signals Mediated by Distinct Regions of the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor Receptor</title><title>Blood</title><addtitle>Blood</addtitle><description>Granulocyte colony-stimulating factor receptor (G-CSFR) regulates the proliferation and differentiation of neutrophilic progenitor cells through interaction with its cytokine. Exposure of WEHI-3B D+ myelomonocytic leukemia and myeloid LGM-1 cells overexpressing the G-CSFR to G-CSF resulted in induction of differentiation as measured by (1) the ability to reduce nitroblue tetrazolium (NBT), (2) the expression of Mac-I antigen, and (3) the expression of FcγII/III receptor. Mutational analyses indicated that distinct regions of the cytoplasmic domain were critical for efficient induction of each functional marker. The membrane proximal region containing homology sequences of boxes 1 and 2 was important for the activation of all three functional markers of mature neutrophils. Induction of the capacities to express Mac-I antigen or FcγII/III receptor also required additional sequences in the membrane proximal region between amino acids 70 and 100 and may be dependent on the phosphorylation of Tyr703. The findings suggest that distinct sequences within the amino-terminal region of the cytoplasmic domain of the receptor are sufficient to induce these functional markers of differentiation, and receptor tyrosine phosphorylation may be necessary.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>DNA Mutational Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulocyte Colony-Stimulating Factor - metabolism</subject><subject>Granulocyte Colony-Stimulating Factor - pharmacology</subject><subject>Macrophage-1 Antigen - biosynthesis</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Receptors, Granulocyte Colony-Stimulating Factor - genetics</subject><subject>Receptors, Granulocyte Colony-Stimulating Factor - metabolism</subject><subject>Receptors, IgG - biosynthesis</subject><subject>Signal Transduction - drug effects</subject><subject>Tumor Cells, Cultured</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcGO0zAQhi0EYsvCA3BBPqC9pYxjJ07ECXXpgrQIiQWulmtPipETF9tBylPwyrjbIjhxsmf8_f9Y8xPynMGasa5-tfMh2PXXnpdyzaUUD8iKNXVXAdTwkKwAoK1EL9kFeZLSdwAmeN08JhcMOO9Fx1fk13aeTHZh0p5eu2HAiFN2-tihd25f2ol-QFs6aOluKUzKrijoJ9wXJtEw0PwN6WbJ4eB1Gp2h12HUbvrzchP1NPtgllyo4MO0VHfZjbMvQ6Y93WqTQyx2Bg_l8pQ8GspMfHY-L8mX7dvPm3fV7ceb95s3t5Wp64ZXvGFt38rB6p5LEKZD1lgGgEzaTiDsWiuFtKyTFsA0CG3DhbU91KYXorX8klydfA8x_JgxZTW6ZNB7PWGYk2p7KWvRyAKyE2hiSCnioA7RjTouioE6pqDuU1AlhVKqYwpF8-JsPu9GtP8oTmsvwMszoJPRfigrMi795TrO4H726xOGZRM_HUaVjMPJlDwimqxscP_5xW8Om6b4</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Koay, Debbie C.</creator><creator>Sartorelli, Alan C.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Functional Differentiation Signals Mediated by Distinct Regions of the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor Receptor</title><author>Koay, Debbie C. ; Sartorelli, Alan C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2253-3516967fda93704c8e15d100e17d84e0b6d747d187d00c5e06534dd902c9446d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>DNA Mutational Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulocyte Colony-Stimulating Factor - metabolism</topic><topic>Granulocyte Colony-Stimulating Factor - pharmacology</topic><topic>Macrophage-1 Antigen - biosynthesis</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Receptors, Granulocyte Colony-Stimulating Factor - genetics</topic><topic>Receptors, Granulocyte Colony-Stimulating Factor - metabolism</topic><topic>Receptors, IgG - biosynthesis</topic><topic>Signal Transduction - drug effects</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koay, Debbie C.</creatorcontrib><creatorcontrib>Sartorelli, Alan C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koay, Debbie C.</au><au>Sartorelli, Alan C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Differentiation Signals Mediated by Distinct Regions of the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor Receptor</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>93</volume><issue>11</issue><spage>3774</spage><epage>3784</epage><pages>3774-3784</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Granulocyte colony-stimulating factor receptor (G-CSFR) regulates the proliferation and differentiation of neutrophilic progenitor cells through interaction with its cytokine. Exposure of WEHI-3B D+ myelomonocytic leukemia and myeloid LGM-1 cells overexpressing the G-CSFR to G-CSF resulted in induction of differentiation as measured by (1) the ability to reduce nitroblue tetrazolium (NBT), (2) the expression of Mac-I antigen, and (3) the expression of FcγII/III receptor. Mutational analyses indicated that distinct regions of the cytoplasmic domain were critical for efficient induction of each functional marker. The membrane proximal region containing homology sequences of boxes 1 and 2 was important for the activation of all three functional markers of mature neutrophils. Induction of the capacities to express Mac-I antigen or FcγII/III receptor also required additional sequences in the membrane proximal region between amino acids 70 and 100 and may be dependent on the phosphorylation of Tyr703. The findings suggest that distinct sequences within the amino-terminal region of the cytoplasmic domain of the receptor are sufficient to induce these functional markers of differentiation, and receptor tyrosine phosphorylation may be necessary.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>10339483</pmid><doi>10.1182/blood.V93.11.3774</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological and medical sciences Cell Differentiation Cell differentiation, maturation, development, hematopoiesis Cell physiology DNA Mutational Analysis Fundamental and applied biological sciences. Psychology Granulocyte Colony-Stimulating Factor - metabolism Granulocyte Colony-Stimulating Factor - pharmacology Macrophage-1 Antigen - biosynthesis Mice Molecular and cellular biology Receptors, Granulocyte Colony-Stimulating Factor - genetics Receptors, Granulocyte Colony-Stimulating Factor - metabolism Receptors, IgG - biosynthesis Signal Transduction - drug effects Tumor Cells, Cultured |
title | Functional Differentiation Signals Mediated by Distinct Regions of the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor Receptor |
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