Structural Basis for T-antigen Hydrolysis by Streptococcus pneumoniae: A TARGET FOR STRUCTURE-BASED VACCINE DESIGN
Streptococcus pneumoniae endo-α-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase from family GH101 involved in the breakdown of mucin type O-linked glycans. The 189-kDa mature enzyme specifically hydrolyzes the T-antigen disaccharide from extracellular host glycoproteins and...
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| Veröffentlicht in: | The Journal of biological chemistry 2008-11, Vol.283 (46), p.31279-31283 |
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| container_issue | 46 |
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| container_title | The Journal of biological chemistry |
| container_volume | 283 |
| creator | Caines, Matthew E.C Zhu, Haizhong Vuckovic, Marija Willis, Lisa M Withers, Stephen G Wakarchuk, Warren W Strynadka, Natalie C.J |
| description | Streptococcus pneumoniae endo-α-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase from family GH101 involved in the breakdown of mucin type O-linked glycans. The 189-kDa mature enzyme specifically hydrolyzes the T-antigen disaccharide from extracellular host glycoproteins and is representative of a broadly important class of virulence factors that have remained structurally uncharacterized due to their large size and highly modular nature. Here we report a 2.9 Å resolution crystal structure that remarkably captures the multidomain architecture and characterizes a catalytic center unexpectedly resembling that of α-amylases. Our analysis presents a complete model of glycoprotein recognition and provides a basis for the structure-based design of novel Streptococcus vaccines and therapeutics. |
| doi_str_mv | 10.1074/jbc.C800150200 |
| format | Article |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Antigens, Viral, Tumor - chemistry Antigens, Viral, Tumor - immunology Antigens, Viral, Tumor - metabolism Catalytic Domain Crystallography, X-Ray Drug Design Hydrolysis Models, Molecular Protein Structure, Tertiary Streptococcal Vaccines - immunology Streptococcus pneumoniae - chemistry Streptococcus pneumoniae - immunology Streptococcus pneumoniae - metabolism |
| title | Structural Basis for T-antigen Hydrolysis by Streptococcus pneumoniae: A TARGET FOR STRUCTURE-BASED VACCINE DESIGN |
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