Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine
In this study, we provide evidence that a recombinant fusion protein containing β-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against...
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| Veröffentlicht in: | Viral immunology 1999, Vol.12 (1), p.1-8 |
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| creator | Huang, H Yang, Z Xu, Q Sheng, Z Xie, Y Yan, W You, Y Sun, L Zheng, Z |
| description | In this study, we provide evidence that a recombinant fusion protein containing β-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge. |
| doi_str_mv | 10.1089/vim.1999.12.1 |
| format | Article |
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Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. 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Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.</description><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Aphthovirus - genetics</subject><subject>Aphthovirus - immunology</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - immunology</subject><subject>Foot-and-Mouth Disease - prevention & control</subject><subject>Foot-and-mouth disease virus</subject><subject>Guinea Pigs</subject><subject>Immunity, Cellular - genetics</subject><subject>Peptides - genetics</subject><subject>Peptides - immunology</subject><subject>Plasmids - immunology</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Swine</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Viral Vaccines - genetics</subject><subject>Viral Vaccines - immunology</subject><issn>0882-8245</issn><issn>1557-8976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQRq2Kqiy0x16RT9yyeOzYiY8rYGGlbYta4Go53sniamNTOynqvyfZ5dAbp9HMvHmH-Qj5CmwOrNYXf303B631HPgcPpAZSFkVta7UEZmxuuZFzUt5TE5y_s0Yq1UtPpFjYEIILqoZST_Rxa7xwYaeLofsY6B3KfboA7VhQ6--L-ijdc4HzHSxtT7kkYux32-_xaF_olc-o81IH30aMl2FFl0_eUbFzTAeWnrnt3v-18vYfiYfW7vL-OWtnpKH5fX95W2x_nGzulysCydU2RfQaNk6Blw5V8oNoBRKqqZtlAYn0SqLzMnKSb6RCp2AUjCtsW20YjU0VpyS84P3OcU_A-bedD473O1swDhko8YnVZzzd0GouJQlhxEsDqBLMeeErXlOvrPpnwFmpjTMmIaZ0jDAzcSfvYmHpsPNf_Th_SMgDsA0tiHsPDaY-ne0rysHlwE</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Huang, H</creator><creator>Yang, Z</creator><creator>Xu, Q</creator><creator>Sheng, Z</creator><creator>Xie, Y</creator><creator>Yan, W</creator><creator>You, Y</creator><creator>Sun, L</creator><creator>Zheng, Z</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine</title><author>Huang, H ; Yang, Z ; Xu, Q ; Sheng, Z ; Xie, Y ; Yan, W ; You, Y ; Sun, L ; Zheng, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-1b95fc0126cc45d1e53656bfb691c5ea6ae0c57c52d56ec3143099efb96081ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Aphthovirus - genetics</topic><topic>Aphthovirus - immunology</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - immunology</topic><topic>Foot-and-Mouth Disease - prevention & control</topic><topic>Foot-and-mouth disease virus</topic><topic>Guinea Pigs</topic><topic>Immunity, Cellular - genetics</topic><topic>Peptides - genetics</topic><topic>Peptides - immunology</topic><topic>Plasmids - immunology</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Swine</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - immunology</topic><topic>Viral Vaccines - genetics</topic><topic>Viral Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, H</creatorcontrib><creatorcontrib>Yang, Z</creatorcontrib><creatorcontrib>Xu, Q</creatorcontrib><creatorcontrib>Sheng, Z</creatorcontrib><creatorcontrib>Xie, Y</creatorcontrib><creatorcontrib>Yan, W</creatorcontrib><creatorcontrib>You, Y</creatorcontrib><creatorcontrib>Sun, L</creatorcontrib><creatorcontrib>Zheng, Z</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Viral immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, H</au><au>Yang, Z</au><au>Xu, Q</au><au>Sheng, Z</au><au>Xie, Y</au><au>Yan, W</au><au>You, Y</au><au>Sun, L</au><au>Zheng, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine</atitle><jtitle>Viral immunology</jtitle><addtitle>Viral Immunol</addtitle><date>1999</date><risdate>1999</risdate><volume>12</volume><issue>1</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>0882-8245</issn><eissn>1557-8976</eissn><abstract>In this study, we provide evidence that a recombinant fusion protein containing β-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. 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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Animals Antibodies, Viral - biosynthesis Aphthovirus - genetics Aphthovirus - immunology beta-Galactosidase - genetics beta-Galactosidase - immunology Foot-and-Mouth Disease - prevention & control Foot-and-mouth disease virus Guinea Pigs Immunity, Cellular - genetics Peptides - genetics Peptides - immunology Plasmids - immunology Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Swine Vaccines, DNA - genetics Vaccines, DNA - immunology Viral Proteins - genetics Viral Proteins - immunology Viral Vaccines - genetics Viral Vaccines - immunology |
| title | Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine |
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