Establishment of a New System for Determination of Coreceptor Usages of HIV Based on the Human Glioma NP-2 Cell Line
CD4 and one of the G-protein-coupled receptors (GPCRs) on the cell surface function as a receptor and a coreceptor, respectively, in infection of cells with human and simian immunodeficiency viruses (HIV/SIV). To determine which GPCRs can be coreceptors for HIV (HIV-1 and HIV-2) or SIV infection, se...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1999-05, Vol.258 (2), p.313-321 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Soda, Yasushi Shimizu, Nobuaki Jinno, Atsushi Liu, Hui-Yu Kanbe, Katsuaki Kitamura, Toshio Hoshino, Hiroo |
| description | CD4 and one of the G-protein-coupled receptors (GPCRs) on the cell surface function as a receptor and a coreceptor, respectively, in infection of cells with human and simian immunodeficiency viruses (HIV/SIV). To determine which GPCRs can be coreceptors for HIV (HIV-1 and HIV-2) or SIV infection, several cell lines, including human osteosarcoma HOS-T4 cells and human glioma U87/CD4 cells, have been used. However, these cells often show susceptibilities to some HIV or SIV strains before transduction of GPCRs. The results of this study showed that a CD4-transduced human glioma cell line, NP-2/CD4, a human erythroleukemia cell line, K562/CD4, and a human ovarian cancer cell line, TYK/CD4, were completely resistant to the HIV-1 and HIV-2 strains tested. After transduction of several GPCRs into NP-2/CD4, K562/CD4, or TYK/CD4 cells, NP-2/CD4 cells but not K562/CD4 or TYK/CD4 cells mostly showed expected susceptibilities to several HIV strains. Therefore, an NP-2 cell system would be useful to determine the coreceptor usage of HIV isolates, to find a new coreceptor for HIV/SIV, and to analyze the early stages of HIV/SIV infection. |
| doi_str_mv | 10.1006/bbrc.1999.0633 |
| format | Article |
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To determine which GPCRs can be coreceptors for HIV (HIV-1 and HIV-2) or SIV infection, several cell lines, including human osteosarcoma HOS-T4 cells and human glioma U87/CD4 cells, have been used. However, these cells often show susceptibilities to some HIV or SIV strains before transduction of GPCRs. The results of this study showed that a CD4-transduced human glioma cell line, NP-2/CD4, a human erythroleukemia cell line, K562/CD4, and a human ovarian cancer cell line, TYK/CD4, were completely resistant to the HIV-1 and HIV-2 strains tested. After transduction of several GPCRs into NP-2/CD4, K562/CD4, or TYK/CD4 cells, NP-2/CD4 cells but not K562/CD4 or TYK/CD4 cells mostly showed expected susceptibilities to several HIV strains. 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To determine which GPCRs can be coreceptors for HIV (HIV-1 and HIV-2) or SIV infection, several cell lines, including human osteosarcoma HOS-T4 cells and human glioma U87/CD4 cells, have been used. However, these cells often show susceptibilities to some HIV or SIV strains before transduction of GPCRs. The results of this study showed that a CD4-transduced human glioma cell line, NP-2/CD4, a human erythroleukemia cell line, K562/CD4, and a human ovarian cancer cell line, TYK/CD4, were completely resistant to the HIV-1 and HIV-2 strains tested. After transduction of several GPCRs into NP-2/CD4, K562/CD4, or TYK/CD4 cells, NP-2/CD4 cells but not K562/CD4 or TYK/CD4 cells mostly showed expected susceptibilities to several HIV strains. Therefore, an NP-2 cell system would be useful to determine the coreceptor usage of HIV isolates, to find a new coreceptor for HIV/SIV, and to analyze the early stages of HIV/SIV infection.</description><subject>AIDS/HIV</subject><subject>Base Sequence</subject><subject>CD4 Antigens - metabolism</subject><subject>DNA Primers</subject><subject>Glioma - metabolism</subject><subject>Glioma - pathology</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>HIV - metabolism</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Receptors, HIV - metabolism</subject><subject>Simian immunodeficiency virus</subject><subject>Transduction, Genetic</subject><subject>Tumor Cells, Cultured</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9vFCEUB3BibOxavXo0nLzNygPmB0fd1m6TTWuiNd4IMG8sZmZYga3pfy-T7cFL0xMJfN435H0JeQdsDYw1H62Nbg1KqTVrhHhBVsAUqzgw-ZKsWBEVV_DzlLxO6TdjALJRr8gpMMGV6OSK5IuUjR19uptwzjQM1NBr_Eu_PaSMEx1CpOeYMU5-NtmHeRGbENHhPpe322R-YVout1c_6GeTsKcF5Tuk28NkZno5-jCVyK8VpxscR7rzM74hJ4MZE759PM_I7ZeL75tttbu5vNp82lVOijZXnHHLwVmpQBgueTcYkHLgfVdDbc3QDdBDY1FYwV1TSwECWd92vWucLFickQ_H3H0Mfw6Ysp58cuUXZsZwSLpRbVO3Zew5CC2vWa26AtdH6GJIKeKg99FPJj5oYHopRC-F6KUQvRRSBt4_Jh_shP1__NhAAd0RYFnEvceok_M4O-x92XLWffBPZf8DPQSYoQ</recordid><startdate>19990510</startdate><enddate>19990510</enddate><creator>Soda, Yasushi</creator><creator>Shimizu, Nobuaki</creator><creator>Jinno, Atsushi</creator><creator>Liu, Hui-Yu</creator><creator>Kanbe, Katsuaki</creator><creator>Kitamura, Toshio</creator><creator>Hoshino, Hiroo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19990510</creationdate><title>Establishment of a New System for Determination of Coreceptor Usages of HIV Based on the Human Glioma NP-2 Cell Line</title><author>Soda, Yasushi ; Shimizu, Nobuaki ; Jinno, Atsushi ; Liu, Hui-Yu ; Kanbe, Katsuaki ; Kitamura, Toshio ; Hoshino, Hiroo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-202b21cb4913a2428fa144f2d8515baf8f1d16be3b32c654313e0d78dc6c48fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AIDS/HIV</topic><topic>Base Sequence</topic><topic>CD4 Antigens - metabolism</topic><topic>DNA Primers</topic><topic>Glioma - metabolism</topic><topic>Glioma - pathology</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>HIV - metabolism</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Receptors, HIV - metabolism</topic><topic>Simian immunodeficiency virus</topic><topic>Transduction, Genetic</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soda, Yasushi</creatorcontrib><creatorcontrib>Shimizu, Nobuaki</creatorcontrib><creatorcontrib>Jinno, Atsushi</creatorcontrib><creatorcontrib>Liu, Hui-Yu</creatorcontrib><creatorcontrib>Kanbe, Katsuaki</creatorcontrib><creatorcontrib>Kitamura, Toshio</creatorcontrib><creatorcontrib>Hoshino, Hiroo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soda, Yasushi</au><au>Shimizu, Nobuaki</au><au>Jinno, Atsushi</au><au>Liu, Hui-Yu</au><au>Kanbe, Katsuaki</au><au>Kitamura, Toshio</au><au>Hoshino, Hiroo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a New System for Determination of Coreceptor Usages of HIV Based on the Human Glioma NP-2 Cell Line</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1999-05-10</date><risdate>1999</risdate><volume>258</volume><issue>2</issue><spage>313</spage><epage>321</epage><pages>313-321</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>CD4 and one of the G-protein-coupled receptors (GPCRs) on the cell surface function as a receptor and a coreceptor, respectively, in infection of cells with human and simian immunodeficiency viruses (HIV/SIV). To determine which GPCRs can be coreceptors for HIV (HIV-1 and HIV-2) or SIV infection, several cell lines, including human osteosarcoma HOS-T4 cells and human glioma U87/CD4 cells, have been used. However, these cells often show susceptibilities to some HIV or SIV strains before transduction of GPCRs. The results of this study showed that a CD4-transduced human glioma cell line, NP-2/CD4, a human erythroleukemia cell line, K562/CD4, and a human ovarian cancer cell line, TYK/CD4, were completely resistant to the HIV-1 and HIV-2 strains tested. After transduction of several GPCRs into NP-2/CD4, K562/CD4, or TYK/CD4 cells, NP-2/CD4 cells but not K562/CD4 or TYK/CD4 cells mostly showed expected susceptibilities to several HIV strains. 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| subjects | AIDS/HIV Base Sequence CD4 Antigens - metabolism DNA Primers Glioma - metabolism Glioma - pathology GTP-Binding Proteins - metabolism HIV - metabolism Human immunodeficiency virus Humans Receptors, HIV - metabolism Simian immunodeficiency virus Transduction, Genetic Tumor Cells, Cultured |
| title | Establishment of a New System for Determination of Coreceptor Usages of HIV Based on the Human Glioma NP-2 Cell Line |
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