Leishmania ( Viannia) braziliensis: New primers for identification using polymerase chain reaction
The objective of this study was to develop specific primers for Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various L. ( V.) braziliensis serodemes and various Leishmania species and als...
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| Veröffentlicht in: | Experimental parasitology 2008-12, Vol.120 (4), p.300-305 |
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| creator | Marcussi, V.M. Marcussi, L.M. Barbosa-Tessmann, I.P. Lonardoni, M.V.C. Silveira, T.G.V. |
| description | The objective of this study was to develop specific primers for
Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various
L. (
V.)
braziliensis serodemes and various
Leishmania species and also using
Trypanosoma cruzi. A specific fragment of 536
bp was detected from 50
ng of DNA in a crude extract derived from
L. (
V.)
braziliensis. The DNA fragment was not detected when DNA from other
Leishmania species or from
T. cruzi was used as template in the PCR. Furthermore, when tested with DNA from cutaneous leishmaniasis the designed primers and reaction gave positive results. Taking into consideration that the primers LBF1 and LBR1 could specifically identify
L. (
V.)
braziliensis, they could be considered for use in
L. (
V.)
braziliensis diagnosis and epidemiological studies. |
| doi_str_mv | 10.1016/j.exppara.2008.08.005 |
| format | Article |
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Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various
L. (
V.)
braziliensis serodemes and various
Leishmania species and also using
Trypanosoma cruzi. A specific fragment of 536
bp was detected from 50
ng of DNA in a crude extract derived from
L. (
V.)
braziliensis. The DNA fragment was not detected when DNA from other
Leishmania species or from
T. cruzi was used as template in the PCR. Furthermore, when tested with DNA from cutaneous leishmaniasis the designed primers and reaction gave positive results. Taking into consideration that the primers LBF1 and LBR1 could specifically identify
L. (
V.)
braziliensis, they could be considered for use in
L. (
V.)
braziliensis diagnosis and epidemiological studies.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2008.08.005</identifier><identifier>PMID: 18786532</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; DNA primers ; DNA Primers - chemistry ; DNA Primers - genetics ; DNA, Kinetoplast - chemistry ; DNA, Kinetoplast - isolation & purification ; DNA, Protozoan - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Humans ; Leishmania ; Leishmania - genetics ; Leishmania - isolation & purification ; Leishmania braziliensis ; Leishmania braziliensis - genetics ; Leishmania braziliensis - isolation & purification ; Leishmaniasis ; Life cycle. Host-agent relationship. Pathogenesis ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - standards ; Protozoa ; Sensitivity and Specificity ; Sequence Alignment ; Species Specificity ; Trypanosoma cruzi ; Trypanosoma cruzi - genetics ; Trypanosoma cruzi - isolation & purification ; Viannia braziliensis</subject><ispartof>Experimental parasitology, 2008-12, Vol.120 (4), p.300-305</ispartof><rights>2008 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-cdc403e107ee69ef8c02c6123b0f449fccc013902147ea11038bb11ad3cd6d1c3</citedby><cites>FETCH-LOGICAL-c424t-cdc403e107ee69ef8c02c6123b0f449fccc013902147ea11038bb11ad3cd6d1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2008.08.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20874214$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18786532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marcussi, V.M.</creatorcontrib><creatorcontrib>Marcussi, L.M.</creatorcontrib><creatorcontrib>Barbosa-Tessmann, I.P.</creatorcontrib><creatorcontrib>Lonardoni, M.V.C.</creatorcontrib><creatorcontrib>Silveira, T.G.V.</creatorcontrib><title>Leishmania ( Viannia) braziliensis: New primers for identification using polymerase chain reaction</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>The objective of this study was to develop specific primers for
Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various
L. (
V.)
braziliensis serodemes and various
Leishmania species and also using
Trypanosoma cruzi. A specific fragment of 536
bp was detected from 50
ng of DNA in a crude extract derived from
L. (
V.)
braziliensis. The DNA fragment was not detected when DNA from other
Leishmania species or from
T. cruzi was used as template in the PCR. Furthermore, when tested with DNA from cutaneous leishmaniasis the designed primers and reaction gave positive results. Taking into consideration that the primers LBF1 and LBR1 could specifically identify
L. (
V.)
braziliensis, they could be considered for use in
L. (
V.)
braziliensis diagnosis and epidemiological studies.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>DNA primers</subject><subject>DNA Primers - chemistry</subject><subject>DNA Primers - genetics</subject><subject>DNA, Kinetoplast - chemistry</subject><subject>DNA, Kinetoplast - isolation & purification</subject><subject>DNA, Protozoan - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Leishmania</subject><subject>Leishmania - genetics</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmania braziliensis</subject><subject>Leishmania braziliensis - genetics</subject><subject>Leishmania braziliensis - isolation & purification</subject><subject>Leishmaniasis</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - standards</subject><subject>Protozoa</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Alignment</subject><subject>Species Specificity</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - genetics</subject><subject>Trypanosoma cruzi - isolation & purification</subject><subject>Viannia braziliensis</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhI4B8oYJDlrHjJA4XVFX8k1ZwAa6WM5nQWWWTYGcp5dPjaKNyrDTSWJrfsz3vCfFcwVaBKt_st_RnmnzwWw1gt0tB8UBsFNSQaWPqh2IDoExmbG3OxJMY95BApc1jcaZsZcsi1xvR7Ijj9cEP7OUr-YP9kE6vZRP8X-6ZhsjxrfxCN3IKfKAQZTcGyS0NM3eMfuZxkMfIw085jf1tInwkideeBxnI4zJ_Kh51vo_0bO3n4vuH99-uPmW7rx8_X13uMjTazBm2aCAnBRVRWVNnETSWSucNdGmdDhFB5TVoZSrySkFum0Yp3-bYlq3C_FxcnO6dwvjrSHF2B45Ife8HGo_RlXVVFgWU94LJ0MIWVZ7A4gRiGGMM1LnFBR9unQK3pOD2bk1hEVm3FBRJ92J94NgcqP2vWm1PwMsV8BF93wU_IMc7ToOtTNozce9OHCXffjMFFzGFgtRyIJxdO_I9X_kHXZSpOQ</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Marcussi, V.M.</creator><creator>Marcussi, L.M.</creator><creator>Barbosa-Tessmann, I.P.</creator><creator>Lonardoni, M.V.C.</creator><creator>Silveira, T.G.V.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Leishmania ( Viannia) braziliensis: New primers for identification using polymerase chain reaction</title><author>Marcussi, V.M. ; Marcussi, L.M. ; Barbosa-Tessmann, I.P. ; Lonardoni, M.V.C. ; Silveira, T.G.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-cdc403e107ee69ef8c02c6123b0f449fccc013902147ea11038bb11ad3cd6d1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>DNA primers</topic><topic>DNA Primers - chemistry</topic><topic>DNA Primers - genetics</topic><topic>DNA, Kinetoplast - chemistry</topic><topic>DNA, Kinetoplast - isolation & purification</topic><topic>DNA, Protozoan - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Leishmania</topic><topic>Leishmania - genetics</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmania braziliensis</topic><topic>Leishmania braziliensis - genetics</topic><topic>Leishmania braziliensis - isolation & purification</topic><topic>Leishmaniasis</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - standards</topic><topic>Protozoa</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Alignment</topic><topic>Species Specificity</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - genetics</topic><topic>Trypanosoma cruzi - isolation & purification</topic><topic>Viannia braziliensis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marcussi, V.M.</creatorcontrib><creatorcontrib>Marcussi, L.M.</creatorcontrib><creatorcontrib>Barbosa-Tessmann, I.P.</creatorcontrib><creatorcontrib>Lonardoni, M.V.C.</creatorcontrib><creatorcontrib>Silveira, T.G.V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marcussi, V.M.</au><au>Marcussi, L.M.</au><au>Barbosa-Tessmann, I.P.</au><au>Lonardoni, M.V.C.</au><au>Silveira, T.G.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leishmania ( Viannia) braziliensis: New primers for identification using polymerase chain reaction</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>120</volume><issue>4</issue><spage>300</spage><epage>305</epage><pages>300-305</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>The objective of this study was to develop specific primers for
Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various
L. (
V.)
braziliensis serodemes and various
Leishmania species and also using
Trypanosoma cruzi. A specific fragment of 536
bp was detected from 50
ng of DNA in a crude extract derived from
L. (
V.)
braziliensis. The DNA fragment was not detected when DNA from other
Leishmania species or from
T. cruzi was used as template in the PCR. Furthermore, when tested with DNA from cutaneous leishmaniasis the designed primers and reaction gave positive results. Taking into consideration that the primers LBF1 and LBR1 could specifically identify
L. (
V.)
braziliensis, they could be considered for use in
L. (
V.)
braziliensis diagnosis and epidemiological studies.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>18786532</pmid><doi>10.1016/j.exppara.2008.08.005</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Experimental parasitology, 2008-12, Vol.120 (4), p.300-305 |
| issn | 0014-4894 1090-2449 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences DNA primers DNA Primers - chemistry DNA Primers - genetics DNA, Kinetoplast - chemistry DNA, Kinetoplast - isolation & purification DNA, Protozoan - isolation & purification Fundamental and applied biological sciences. Psychology Humans Leishmania Leishmania - genetics Leishmania - isolation & purification Leishmania braziliensis Leishmania braziliensis - genetics Leishmania braziliensis - isolation & purification Leishmaniasis Life cycle. Host-agent relationship. Pathogenesis Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Protozoa Sensitivity and Specificity Sequence Alignment Species Specificity Trypanosoma cruzi Trypanosoma cruzi - genetics Trypanosoma cruzi - isolation & purification Viannia braziliensis |
| title | Leishmania ( Viannia) braziliensis: New primers for identification using polymerase chain reaction |
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