Inhibition of influenza A virus reproduction by a ribozyme targeted against PB1 mRNA
A ribozyme gene directed at a specific cleavage of mRNA coding for PB1 protein, a component of RNA-dependent RNA-polymerase of influenza A virus, was constructed. The avian adenovirus CELO virus-associated RNA (VA RNA CELO) promoter and human cytomegalovirus (CMV) promoter were used for the permanen...
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| Veröffentlicht in: | Antiviral research 1999-05, Vol.42 (1), p.47-57 |
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| creator | Lazarev, V.N. Shmarov, M.M. Zakhartchouk, A.N. Yurov, G.K. Misurina, O.U. Akopian, T.A. Grinenko, N.F. Grodnitskaya, N.G. Kaverin, N.V. Naroditsky, B.S. |
| description | A ribozyme gene directed at a specific cleavage of mRNA coding for PB1 protein, a component of RNA-dependent RNA-polymerase of influenza A virus, was constructed. The avian adenovirus CELO virus-associated RNA (VA RNA CELO) promoter and human cytomegalovirus (CMV) promoter were used for the permanent expression of the ribozyme in cell lines. The cells were infected with influenza A virus strains A/Singapore/1/57 and A/WSN/33, and the suppression of the virus reproduction and virus-specific protein synthesis was measured. The maximal level of the inhibition of virus reproduction as compared to the reproduction in non-transformed cells was 93.5%. Defective recombinant adenoviruses were constructed carrying the genes of functional and non-functional ribozymes under the control of human cytomegalovirus (CMV) promoter. The reproduction of A/WSN/33 virus in CV-1 cells preinfected with recombinant adenoviruses was shown to be suppressed. |
| doi_str_mv | 10.1016/S0166-3542(99)00015-7 |
| format | Article |
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The avian adenovirus CELO virus-associated RNA (VA RNA CELO) promoter and human cytomegalovirus (CMV) promoter were used for the permanent expression of the ribozyme in cell lines. The cells were infected with influenza A virus strains A/Singapore/1/57 and A/WSN/33, and the suppression of the virus reproduction and virus-specific protein synthesis was measured. The maximal level of the inhibition of virus reproduction as compared to the reproduction in non-transformed cells was 93.5%. Defective recombinant adenoviruses were constructed carrying the genes of functional and non-functional ribozymes under the control of human cytomegalovirus (CMV) promoter. 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The avian adenovirus CELO virus-associated RNA (VA RNA CELO) promoter and human cytomegalovirus (CMV) promoter were used for the permanent expression of the ribozyme in cell lines. The cells were infected with influenza A virus strains A/Singapore/1/57 and A/WSN/33, and the suppression of the virus reproduction and virus-specific protein synthesis was measured. The maximal level of the inhibition of virus reproduction as compared to the reproduction in non-transformed cells was 93.5%. Defective recombinant adenoviruses were constructed carrying the genes of functional and non-functional ribozymes under the control of human cytomegalovirus (CMV) promoter. The reproduction of A/WSN/33 virus in CV-1 cells preinfected with recombinant adenoviruses was shown to be suppressed.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10333142</pmid><doi>10.1016/S0166-3542(99)00015-7</doi><tpages>11</tpages></addata></record> |
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| ispartof | Antiviral research, 1999-05, Vol.42 (1), p.47-57 |
| issn | 0166-3542 1872-9096 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenoviridae - genetics Adenovirus Animals Base Sequence Cell Line Cytomegalovirus - genetics Humans Influenza A Influenza A virus Influenza A virus - genetics Influenza A virus - physiology Molecular Sequence Data Plasmids Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction Ribozyme RNA Replicase - genetics RNA, Catalytic - genetics RNA, Catalytic - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Viral - genetics RNA, Viral - metabolism Transfection Viral Proteins - biosynthesis Viral Proteins - genetics Virus Replication - physiology |
| title | Inhibition of influenza A virus reproduction by a ribozyme targeted against PB1 mRNA |
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