The TNF-α system in heart failure and after heart transplantation; plasma protein levels, mRNA expression, soluble receptors and plasma buffer capacity
Background The two soluble tumour necrosis factor (TNF) receptors (sTNF-R1, sTNF-R2) can bind TNF-α, which is a cytokine with cardiodepressant properties. In heart failure and after heart transplantation, the TNF-α system is unbalanced, due to elevated levels of sTNF receptors. Aim To assess the act...
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| Veröffentlicht in: | European heart journal 1999-06, Vol.20 (11), p.833-840 |
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| creator | van Riemsdijk-van Overbeeke, I.C. Baan, C.C. Niesters, H.G.M. Hesse, C.J. Loonen, E.H.M. Balk, A.H.M.M. Maat, A.P.W.M. Weimar, W. |
| description | Background The two soluble tumour necrosis factor (TNF) receptors (sTNF-R1, sTNF-R2) can bind TNF-α, which is a cytokine with cardiodepressant properties. In heart failure and after heart transplantation, the TNF-α system is unbalanced, due to elevated levels of sTNF receptors. Aim To assess the activity of the TNF-α system in patients with heart failure and after heart transplantation. Methods We measured TNF-αmRNA expression of peripheral blood mononuclear cells, plasma levels of TNF-α and sTNF reverse transcriptase receptors, using polymerase chain reaction and ELISA and performed a TNF-α binding capacity analysis, quantitating the buffer capacity of patients’ plasma. Results In 11 patients with heart failure and in 15 cardiac allograft recipients, the TNF-α mRNA expression was comparable to controls. This level of mRNA was not accompanied by detectable TNF-αplasma levels. Significantly higher sTNF receptors levels were found in patients: (P |
| doi_str_mv | 10.1053/euhj.1998.1478 |
| format | Article |
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In heart failure and after heart transplantation, the TNF-α system is unbalanced, due to elevated levels of sTNF receptors. Aim To assess the activity of the TNF-α system in patients with heart failure and after heart transplantation. Methods We measured TNF-αmRNA expression of peripheral blood mononuclear cells, plasma levels of TNF-α and sTNF reverse transcriptase receptors, using polymerase chain reaction and ELISA and performed a TNF-α binding capacity analysis, quantitating the buffer capacity of patients’ plasma. Results In 11 patients with heart failure and in 15 cardiac allograft recipients, the TNF-α mRNA expression was comparable to controls. This level of mRNA was not accompanied by detectable TNF-αplasma levels. Significantly higher sTNF receptors levels were found in patients: (P<0·001; ANOVA). The TNF-αbinding capacity of patients’ plasma was significantly increased, which led to decreased TNF-αrecovery (P<0·05). Both sTNF receptors showed a linear correlation with serum creatinine (sTNF-RI: r=0·92; sTNF-R2: r=0·82,P<0·001). Conclusions The TNF-αmRNA expression and plasma levels show that the ‘peripheral’ TNF-αsystem is not activated. The high sTNF-receptors levels and their elevated TNF-αbinding capacity, resulting in decreased TNF-α bioavailability, may contribute to an immunosuppressed state in these patients.</description><identifier>ISSN: 0195-668X</identifier><identifier>EISSN: 1522-9645</identifier><identifier>DOI: 10.1053/euhj.1998.1478</identifier><identifier>PMID: 10329082</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Biological Availability ; Creatinine - blood ; Enzyme-Linked Immunosorbent Assay ; Female ; heart failure ; Heart Failure - metabolism ; heart transplantation ; Heart Transplantation - physiology ; Humans ; Male ; Medical sciences ; Middle Aged ; Receptors, Tumor Necrosis Factor - blood ; Receptors, Tumor Necrosis Factor - genetics ; Receptors, Tumor Necrosis Factor - metabolism ; RNA, Messenger - metabolism ; Solubility ; soluble TNF receptors ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the heart ; TNF-α</subject><ispartof>European heart journal, 1999-06, Vol.20 (11), p.833-840</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 The European Society of Cardiology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-6617fe1845e078830f083ec6af126ecba1d54c8e6252a0ad0699439c5905b7323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1798485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10329082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Riemsdijk-van Overbeeke, I.C.</creatorcontrib><creatorcontrib>Baan, C.C.</creatorcontrib><creatorcontrib>Niesters, H.G.M.</creatorcontrib><creatorcontrib>Hesse, C.J.</creatorcontrib><creatorcontrib>Loonen, E.H.M.</creatorcontrib><creatorcontrib>Balk, A.H.M.M.</creatorcontrib><creatorcontrib>Maat, A.P.W.M.</creatorcontrib><creatorcontrib>Weimar, W.</creatorcontrib><title>The TNF-α system in heart failure and after heart transplantation; plasma protein levels, mRNA expression, soluble receptors and plasma buffer capacity</title><title>European heart journal</title><addtitle>Eur Heart J</addtitle><description>Background The two soluble tumour necrosis factor (TNF) receptors (sTNF-R1, sTNF-R2) can bind TNF-α, which is a cytokine with cardiodepressant properties. In heart failure and after heart transplantation, the TNF-α system is unbalanced, due to elevated levels of sTNF receptors. Aim To assess the activity of the TNF-α system in patients with heart failure and after heart transplantation. Methods We measured TNF-αmRNA expression of peripheral blood mononuclear cells, plasma levels of TNF-α and sTNF reverse transcriptase receptors, using polymerase chain reaction and ELISA and performed a TNF-α binding capacity analysis, quantitating the buffer capacity of patients’ plasma. Results In 11 patients with heart failure and in 15 cardiac allograft recipients, the TNF-α mRNA expression was comparable to controls. This level of mRNA was not accompanied by detectable TNF-αplasma levels. Significantly higher sTNF receptors levels were found in patients: (P<0·001; ANOVA). The TNF-αbinding capacity of patients’ plasma was significantly increased, which led to decreased TNF-αrecovery (P<0·05). Both sTNF receptors showed a linear correlation with serum creatinine (sTNF-RI: r=0·92; sTNF-R2: r=0·82,P<0·001). Conclusions The TNF-αmRNA expression and plasma levels show that the ‘peripheral’ TNF-αsystem is not activated. The high sTNF-receptors levels and their elevated TNF-αbinding capacity, resulting in decreased TNF-α bioavailability, may contribute to an immunosuppressed state in these patients.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biological Availability</subject><subject>Creatinine - blood</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>heart failure</subject><subject>Heart Failure - metabolism</subject><subject>heart transplantation</subject><subject>Heart Transplantation - physiology</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Receptors, Tumor Necrosis Factor - blood</subject><subject>Receptors, Tumor Necrosis Factor - genetics</subject><subject>Receptors, Tumor Necrosis Factor - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Solubility</subject><subject>soluble TNF receptors</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the heart</subject><subject>TNF-α</subject><issn>0195-668X</issn><issn>1522-9645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU1uFDEQhS0EIkNgyxJ5gVilB_-03bZYRYEwoCgENEhRNpbbU9Z06L_YbpS5CdfgIpwJD9MCVi65vvfsqofQc0qWlAj-Gqbt7ZJqrZa0rNQDtKCCsULLUjxEC0K1KKRU10foSYy3hBAlqXyMjijhTBPFFujHegt4fXle_PqJ4y4m6HDT4y3YkLC3TTsFwLbfYOsThPk-BdvHsbV9sqkZ-jc417GzeAxDgqxu4Tu08QR3Xy5PMdyPAWLM3AmOQzvVLeAADsY0hPjHelbXk_f5CWdH65q0e4oeedtGeDafx-jr-bv12aq4-PT-w9npReE4JylPRysPVJUCSKUUJ54oDk5aT5kEV1u6EaVTIJlgltgNkVqXXDuhiagrzvgxenXwzb-_myAm0zXRQZvHg2GKRupKMqlVBpcH0IUhxgDejKHpbNgZSsw-C7PPwuyzMPsssuDF7DzVHWz-ww_Lz8DLGbDR2dbntbom_uMqrUolMlYcsCbHc_-3bcM3IyteCbO6vjE38vNKXr39aK74b8R5pEU</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>van Riemsdijk-van Overbeeke, I.C.</creator><creator>Baan, C.C.</creator><creator>Niesters, H.G.M.</creator><creator>Hesse, C.J.</creator><creator>Loonen, E.H.M.</creator><creator>Balk, A.H.M.M.</creator><creator>Maat, A.P.W.M.</creator><creator>Weimar, W.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>The TNF-α system in heart failure and after heart transplantation; plasma protein levels, mRNA expression, soluble receptors and plasma buffer capacity</title><author>van Riemsdijk-van Overbeeke, I.C. ; Baan, C.C. ; Niesters, H.G.M. ; Hesse, C.J. ; Loonen, E.H.M. ; Balk, A.H.M.M. ; Maat, A.P.W.M. ; Weimar, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-6617fe1845e078830f083ec6af126ecba1d54c8e6252a0ad0699439c5905b7323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biological Availability</topic><topic>Creatinine - blood</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>heart failure</topic><topic>Heart Failure - metabolism</topic><topic>heart transplantation</topic><topic>Heart Transplantation - physiology</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Receptors, Tumor Necrosis Factor - blood</topic><topic>Receptors, Tumor Necrosis Factor - genetics</topic><topic>Receptors, Tumor Necrosis Factor - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Solubility</topic><topic>soluble TNF receptors</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the heart</topic><topic>TNF-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Riemsdijk-van Overbeeke, I.C.</creatorcontrib><creatorcontrib>Baan, C.C.</creatorcontrib><creatorcontrib>Niesters, H.G.M.</creatorcontrib><creatorcontrib>Hesse, C.J.</creatorcontrib><creatorcontrib>Loonen, E.H.M.</creatorcontrib><creatorcontrib>Balk, A.H.M.M.</creatorcontrib><creatorcontrib>Maat, A.P.W.M.</creatorcontrib><creatorcontrib>Weimar, W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European heart journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Riemsdijk-van Overbeeke, I.C.</au><au>Baan, C.C.</au><au>Niesters, H.G.M.</au><au>Hesse, C.J.</au><au>Loonen, E.H.M.</au><au>Balk, A.H.M.M.</au><au>Maat, A.P.W.M.</au><au>Weimar, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The TNF-α system in heart failure and after heart transplantation; plasma protein levels, mRNA expression, soluble receptors and plasma buffer capacity</atitle><jtitle>European heart journal</jtitle><addtitle>Eur Heart J</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>20</volume><issue>11</issue><spage>833</spage><epage>840</epage><pages>833-840</pages><issn>0195-668X</issn><eissn>1522-9645</eissn><abstract>Background The two soluble tumour necrosis factor (TNF) receptors (sTNF-R1, sTNF-R2) can bind TNF-α, which is a cytokine with cardiodepressant properties. In heart failure and after heart transplantation, the TNF-α system is unbalanced, due to elevated levels of sTNF receptors. Aim To assess the activity of the TNF-α system in patients with heart failure and after heart transplantation. Methods We measured TNF-αmRNA expression of peripheral blood mononuclear cells, plasma levels of TNF-α and sTNF reverse transcriptase receptors, using polymerase chain reaction and ELISA and performed a TNF-α binding capacity analysis, quantitating the buffer capacity of patients’ plasma. Results In 11 patients with heart failure and in 15 cardiac allograft recipients, the TNF-α mRNA expression was comparable to controls. This level of mRNA was not accompanied by detectable TNF-αplasma levels. Significantly higher sTNF receptors levels were found in patients: (P<0·001; ANOVA). The TNF-αbinding capacity of patients’ plasma was significantly increased, which led to decreased TNF-αrecovery (P<0·05). Both sTNF receptors showed a linear correlation with serum creatinine (sTNF-RI: r=0·92; sTNF-R2: r=0·82,P<0·001). Conclusions The TNF-αmRNA expression and plasma levels show that the ‘peripheral’ TNF-αsystem is not activated. The high sTNF-receptors levels and their elevated TNF-αbinding capacity, resulting in decreased TNF-α bioavailability, may contribute to an immunosuppressed state in these patients.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10329082</pmid><doi>10.1053/euhj.1998.1478</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European heart journal, 1999-06, Vol.20 (11), p.833-840 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aged Biological and medical sciences Biological Availability Creatinine - blood Enzyme-Linked Immunosorbent Assay Female heart failure Heart Failure - metabolism heart transplantation Heart Transplantation - physiology Humans Male Medical sciences Middle Aged Receptors, Tumor Necrosis Factor - blood Receptors, Tumor Necrosis Factor - genetics Receptors, Tumor Necrosis Factor - metabolism RNA, Messenger - metabolism Solubility soluble TNF receptors Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the heart TNF-α |
| title | The TNF-α system in heart failure and after heart transplantation; plasma protein levels, mRNA expression, soluble receptors and plasma buffer capacity |
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