Multipotent Stem/Progenitor Cells with Similar Properties Arise from Two Neurogenic Regions of Adult Human Brain
Recentin vitrostudies have shown that the periventricular subependymal zone (SEZ) of the rodent brain is capable ofde novogeneration of neurons and glia. There is less information available on neurogenesis in the adult human brain, and no study has shown the clonal generation of neurons and glia fro...
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Veröffentlicht in: | Experimental neurology 1999-04, Vol.156 (2), p.333-344 |
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description | Recentin vitrostudies have shown that the periventricular subependymal zone (SEZ) of the rodent brain is capable ofde novogeneration of neurons and glia. There is less information available on neurogenesis in the adult human brain, and no study has shown the clonal generation of neurons and glia fromin vitro-generated “neurospheres.” Here we describe the isolation of proliferative stem/progenitor cells within neurospheres from two different regions, the SEZ and the hippocampus, from surgical biopsy specimens of adult (24–57 years) human brain. Using light and electron microscopy; immunocytochemistry for a variety of neuronal, glial, and developmental (including extracellular matrix; ECM) markers; and the reverse transcriptase polymerase chain reaction to demonstrate different gene transcripts found in neurospheres, it is shown that the adult human brain harbors a complex population of stem/progenitor cells that can generate neuronal and glial progeny under particularin vitrogrowth conditions. These methods also show that these neurospheres contain both neurons and glia and demonstrate regional similarities at the mRNA level, indicating common stem/progenitor cell types within two different neurogenic regions of the adult human brain. In addition to the synthesis of developmentally regulated molecules such as the ECM protein tenascin-C, a variety of other genes (e.g.,Pax 6) and proteins (e.g., Bcl-2) involved in cell survival and differentiation are expressed by adult human brain neurospheres. |
doi_str_mv | 10.1006/exnr.1999.7028 |
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There is less information available on neurogenesis in the adult human brain, and no study has shown the clonal generation of neurons and glia fromin vitro-generated “neurospheres.” Here we describe the isolation of proliferative stem/progenitor cells within neurospheres from two different regions, the SEZ and the hippocampus, from surgical biopsy specimens of adult (24–57 years) human brain. Using light and electron microscopy; immunocytochemistry for a variety of neuronal, glial, and developmental (including extracellular matrix; ECM) markers; and the reverse transcriptase polymerase chain reaction to demonstrate different gene transcripts found in neurospheres, it is shown that the adult human brain harbors a complex population of stem/progenitor cells that can generate neuronal and glial progeny under particularin vitrogrowth conditions. These methods also show that these neurospheres contain both neurons and glia and demonstrate regional similarities at the mRNA level, indicating common stem/progenitor cell types within two different neurogenic regions of the adult human brain. In addition to the synthesis of developmentally regulated molecules such as the ECM protein tenascin-C, a variety of other genes (e.g.,Pax 6) and proteins (e.g., Bcl-2) involved in cell survival and differentiation are expressed by adult human brain neurospheres.</description><identifier>ISSN: 0014-4886</identifier><identifier>EISSN: 1090-2430</identifier><identifier>DOI: 10.1006/exnr.1999.7028</identifier><identifier>PMID: 10328940</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; adult human brain ; Aged ; Aged, 80 and over ; Biomarkers ; Brain - cytology ; Cell Lineage ; Cells, Cultured ; developmental genes ; DNA-Binding Proteins - analysis ; DNA-Binding Proteins - genetics ; extracellular matrix ; Eye Proteins ; Female ; Gene Expression Regulation, Developmental ; Glial Fibrillary Acidic Protein - analysis ; Glial Fibrillary Acidic Protein - genetics ; Hippocampus - cytology ; Homeodomain Proteins ; Humans ; Intermediate Filament Proteins - analysis ; Intermediate Filament Proteins - genetics ; Male ; Middle Aged ; multipotent stem and progenitor cells ; Nerve Tissue Proteins - analysis ; Nerve Tissue Proteins - genetics ; Nestin ; Neurofilament Proteins - analysis ; Neurofilament Proteins - genetics ; Neuroglia - cytology ; Neurons - cytology ; neurons and glia ; neurospheres ; Paired Box Transcription Factors ; PAX6 Transcription Factor ; Phosphopyruvate Hydratase - analysis ; Phosphopyruvate Hydratase - genetics ; Repressor Proteins ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Stem Cells - cytology ; Tenascin - analysis ; Tenascin - genetics</subject><ispartof>Experimental neurology, 1999-04, Vol.156 (2), p.333-344</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-928250ffd0ed138d3e7e9dc638e56c3f1cd9a76eface21b8d2ad8a1f8ab325293</citedby><cites>FETCH-LOGICAL-c272t-928250ffd0ed138d3e7e9dc638e56c3f1cd9a76eface21b8d2ad8a1f8ab325293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/exnr.1999.7028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10328940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kukekov, V.G.</creatorcontrib><creatorcontrib>Laywell, E.D.</creatorcontrib><creatorcontrib>Suslov, O.</creatorcontrib><creatorcontrib>Davies, K.</creatorcontrib><creatorcontrib>Scheffler, B.</creatorcontrib><creatorcontrib>Thomas, L.B.</creatorcontrib><creatorcontrib>O'Brien, T.F.</creatorcontrib><creatorcontrib>Kusakabe, M.</creatorcontrib><creatorcontrib>Steindler, D.A.</creatorcontrib><title>Multipotent Stem/Progenitor Cells with Similar Properties Arise from Two Neurogenic Regions of Adult Human Brain</title><title>Experimental neurology</title><addtitle>Exp Neurol</addtitle><description>Recentin vitrostudies have shown that the periventricular subependymal zone (SEZ) of the rodent brain is capable ofde novogeneration of neurons and glia. There is less information available on neurogenesis in the adult human brain, and no study has shown the clonal generation of neurons and glia fromin vitro-generated “neurospheres.” Here we describe the isolation of proliferative stem/progenitor cells within neurospheres from two different regions, the SEZ and the hippocampus, from surgical biopsy specimens of adult (24–57 years) human brain. Using light and electron microscopy; immunocytochemistry for a variety of neuronal, glial, and developmental (including extracellular matrix; ECM) markers; and the reverse transcriptase polymerase chain reaction to demonstrate different gene transcripts found in neurospheres, it is shown that the adult human brain harbors a complex population of stem/progenitor cells that can generate neuronal and glial progeny under particularin vitrogrowth conditions. These methods also show that these neurospheres contain both neurons and glia and demonstrate regional similarities at the mRNA level, indicating common stem/progenitor cell types within two different neurogenic regions of the adult human brain. In addition to the synthesis of developmentally regulated molecules such as the ECM protein tenascin-C, a variety of other genes (e.g.,Pax 6) and proteins (e.g., Bcl-2) involved in cell survival and differentiation are expressed by adult human brain neurospheres.</description><subject>Adolescent</subject><subject>Adult</subject><subject>adult human brain</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers</subject><subject>Brain - cytology</subject><subject>Cell Lineage</subject><subject>Cells, Cultured</subject><subject>developmental genes</subject><subject>DNA-Binding Proteins - analysis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>extracellular matrix</subject><subject>Eye Proteins</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Glial Fibrillary Acidic Protein - analysis</subject><subject>Glial Fibrillary Acidic Protein - genetics</subject><subject>Hippocampus - cytology</subject><subject>Homeodomain Proteins</subject><subject>Humans</subject><subject>Intermediate Filament Proteins - analysis</subject><subject>Intermediate Filament Proteins - genetics</subject><subject>Male</subject><subject>Middle Aged</subject><subject>multipotent stem and progenitor cells</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nestin</subject><subject>Neurofilament Proteins - analysis</subject><subject>Neurofilament Proteins - genetics</subject><subject>Neuroglia - cytology</subject><subject>Neurons - cytology</subject><subject>neurons and glia</subject><subject>neurospheres</subject><subject>Paired Box Transcription Factors</subject><subject>PAX6 Transcription Factor</subject><subject>Phosphopyruvate Hydratase - analysis</subject><subject>Phosphopyruvate Hydratase - genetics</subject><subject>Repressor Proteins</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Stem Cells - cytology</subject><subject>Tenascin - analysis</subject><subject>Tenascin - genetics</subject><issn>0014-4886</issn><issn>1090-2430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1v2zAURYkiQeOkXTsWnLLJeaRkiRxdI19AmgSxOxM0-ZiykESVpJL031eGMnTp9IZ37gXuIeQLgyUDqC_wrY9LJqVcNsDFB7JgIKHgVQlHZAHAqqISoj4hpyn9AgBZ8eYjOWFQciErWJDh-9hmP4SMfabbjN3FYwzP2PscIt1g2yb66vNPuvWdb3Wk03fAmD0muo4-IXUxdHT3Gug9jnPS0Cd89qFPNDi6tlM_vRk73dNvUfv-Ezl2uk34-f2ekR9Xl7vNTXH3cH27Wd8Vhjc8F5ILvgLnLKBlpbAlNiitqUuBq9qUjhkrdVOj0wY52wvLtRWaOaH3JV9xWZ6R87l3iOH3iCmrziczDdI9hjGpWjY1A7aawOUMmhhSiujUEH2n4x_FQB0cq4NjdXCsDo6nwNf35nHfof0Hn6VOgJgBnPa9eIwqGY-9Qesjmqxs8P_r_gsqU42b</recordid><startdate>199904</startdate><enddate>199904</enddate><creator>Kukekov, V.G.</creator><creator>Laywell, E.D.</creator><creator>Suslov, O.</creator><creator>Davies, K.</creator><creator>Scheffler, B.</creator><creator>Thomas, L.B.</creator><creator>O'Brien, T.F.</creator><creator>Kusakabe, M.</creator><creator>Steindler, D.A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199904</creationdate><title>Multipotent Stem/Progenitor Cells with Similar Properties Arise from Two Neurogenic Regions of Adult Human Brain</title><author>Kukekov, V.G. ; Laywell, E.D. ; Suslov, O. ; Davies, K. ; Scheffler, B. ; Thomas, L.B. ; O'Brien, T.F. ; Kusakabe, M. ; Steindler, D.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-928250ffd0ed138d3e7e9dc638e56c3f1cd9a76eface21b8d2ad8a1f8ab325293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>adult human brain</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers</topic><topic>Brain - cytology</topic><topic>Cell Lineage</topic><topic>Cells, Cultured</topic><topic>developmental genes</topic><topic>DNA-Binding Proteins - analysis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>extracellular matrix</topic><topic>Eye Proteins</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Glial Fibrillary Acidic Protein - analysis</topic><topic>Glial Fibrillary Acidic Protein - genetics</topic><topic>Hippocampus - cytology</topic><topic>Homeodomain Proteins</topic><topic>Humans</topic><topic>Intermediate Filament Proteins - analysis</topic><topic>Intermediate Filament Proteins - genetics</topic><topic>Male</topic><topic>Middle Aged</topic><topic>multipotent stem and progenitor cells</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nestin</topic><topic>Neurofilament Proteins - analysis</topic><topic>Neurofilament Proteins - genetics</topic><topic>Neuroglia - cytology</topic><topic>Neurons - cytology</topic><topic>neurons and glia</topic><topic>neurospheres</topic><topic>Paired Box Transcription Factors</topic><topic>PAX6 Transcription Factor</topic><topic>Phosphopyruvate Hydratase - analysis</topic><topic>Phosphopyruvate Hydratase - genetics</topic><topic>Repressor Proteins</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Stem Cells - cytology</topic><topic>Tenascin - analysis</topic><topic>Tenascin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kukekov, V.G.</creatorcontrib><creatorcontrib>Laywell, E.D.</creatorcontrib><creatorcontrib>Suslov, O.</creatorcontrib><creatorcontrib>Davies, K.</creatorcontrib><creatorcontrib>Scheffler, B.</creatorcontrib><creatorcontrib>Thomas, L.B.</creatorcontrib><creatorcontrib>O'Brien, T.F.</creatorcontrib><creatorcontrib>Kusakabe, M.</creatorcontrib><creatorcontrib>Steindler, D.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kukekov, V.G.</au><au>Laywell, E.D.</au><au>Suslov, O.</au><au>Davies, K.</au><au>Scheffler, B.</au><au>Thomas, L.B.</au><au>O'Brien, T.F.</au><au>Kusakabe, M.</au><au>Steindler, D.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multipotent Stem/Progenitor Cells with Similar Properties Arise from Two Neurogenic Regions of Adult Human Brain</atitle><jtitle>Experimental neurology</jtitle><addtitle>Exp Neurol</addtitle><date>1999-04</date><risdate>1999</risdate><volume>156</volume><issue>2</issue><spage>333</spage><epage>344</epage><pages>333-344</pages><issn>0014-4886</issn><eissn>1090-2430</eissn><abstract>Recentin vitrostudies have shown that the periventricular subependymal zone (SEZ) of the rodent brain is capable ofde novogeneration of neurons and glia. There is less information available on neurogenesis in the adult human brain, and no study has shown the clonal generation of neurons and glia fromin vitro-generated “neurospheres.” Here we describe the isolation of proliferative stem/progenitor cells within neurospheres from two different regions, the SEZ and the hippocampus, from surgical biopsy specimens of adult (24–57 years) human brain. Using light and electron microscopy; immunocytochemistry for a variety of neuronal, glial, and developmental (including extracellular matrix; ECM) markers; and the reverse transcriptase polymerase chain reaction to demonstrate different gene transcripts found in neurospheres, it is shown that the adult human brain harbors a complex population of stem/progenitor cells that can generate neuronal and glial progeny under particularin vitrogrowth conditions. These methods also show that these neurospheres contain both neurons and glia and demonstrate regional similarities at the mRNA level, indicating common stem/progenitor cell types within two different neurogenic regions of the adult human brain. In addition to the synthesis of developmentally regulated molecules such as the ECM protein tenascin-C, a variety of other genes (e.g.,Pax 6) and proteins (e.g., Bcl-2) involved in cell survival and differentiation are expressed by adult human brain neurospheres.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10328940</pmid><doi>10.1006/exnr.1999.7028</doi><tpages>12</tpages></addata></record> |
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subjects | Adolescent Adult adult human brain Aged Aged, 80 and over Biomarkers Brain - cytology Cell Lineage Cells, Cultured developmental genes DNA-Binding Proteins - analysis DNA-Binding Proteins - genetics extracellular matrix Eye Proteins Female Gene Expression Regulation, Developmental Glial Fibrillary Acidic Protein - analysis Glial Fibrillary Acidic Protein - genetics Hippocampus - cytology Homeodomain Proteins Humans Intermediate Filament Proteins - analysis Intermediate Filament Proteins - genetics Male Middle Aged multipotent stem and progenitor cells Nerve Tissue Proteins - analysis Nerve Tissue Proteins - genetics Nestin Neurofilament Proteins - analysis Neurofilament Proteins - genetics Neuroglia - cytology Neurons - cytology neurons and glia neurospheres Paired Box Transcription Factors PAX6 Transcription Factor Phosphopyruvate Hydratase - analysis Phosphopyruvate Hydratase - genetics Repressor Proteins Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Stem Cells - cytology Tenascin - analysis Tenascin - genetics |
title | Multipotent Stem/Progenitor Cells with Similar Properties Arise from Two Neurogenic Regions of Adult Human Brain |
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