Palmitoyl-protein thioesterase, an enzyme implicated in neurodegeneration, is localized in neurons and is developmentally regulated in rat brain
Palmitoyl-protein thioesterase (PPT) is an enzyme involved in cleavage of palmitate residues from acylated proteins. Mutations in PPT gene cause a severe neurodegenerative disorder, infantile neuronal ceroid-lipofuscinosis (INCL), characterized by loss of cortical neurons. In order to clarify the ro...
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| Veröffentlicht in: | Neuroscience letters 1999-04, Vol.265 (1), p.53-56 |
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| creator | Suopanki, Jaana Tyynelä, Jaana Baumann, Marc Haltia, Matti |
| description | Palmitoyl-protein thioesterase (PPT) is an enzyme involved in cleavage of palmitate residues from acylated proteins. Mutations in PPT gene cause a severe neurodegenerative disorder, infantile neuronal ceroid-lipofuscinosis (INCL), characterized by loss of cortical neurons. In order to clarify the role of PPT and palmitoylation/depalmitoylation in the development of CNS and pathogenesis of infantile neuronal ceroid-lipofuscinosis (INCL), we studied the localization and expression of PPT in developing rats. Using immunohistochemical methods, we show for the first time that PPT is truly localized in neurons. Further, using RT-PCR and Western blotting, we show that expression of PPT in rat brain is developmentally regulated, with increasing expression during the maturation of CNS, reaching the maximum in young adulthood. The presented data support the view of PPT being essential for both development and maintenance of cortical neurons. |
| doi_str_mv | 10.1016/S0304-3940(99)00207-4 |
| format | Article |
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Mutations in PPT gene cause a severe neurodegenerative disorder, infantile neuronal ceroid-lipofuscinosis (INCL), characterized by loss of cortical neurons. In order to clarify the role of PPT and palmitoylation/depalmitoylation in the development of CNS and pathogenesis of infantile neuronal ceroid-lipofuscinosis (INCL), we studied the localization and expression of PPT in developing rats. Using immunohistochemical methods, we show for the first time that PPT is truly localized in neurons. Further, using RT-PCR and Western blotting, we show that expression of PPT in rat brain is developmentally regulated, with increasing expression during the maturation of CNS, reaching the maximum in young adulthood. The presented data support the view of PPT being essential for both development and maintenance of cortical neurons.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(99)00207-4</identifier><identifier>PMID: 10327204</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Brain development ; Development. Senescence. Regeneration. Transplantation ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - physiology ; Immunohistochemistry ; Infantile neuronal ceroid-lipofuscinosis ; Nerve Degeneration ; Neurons - chemistry ; Palmitoyl-protein thioesterase ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; RT-PCR, protein expression ; Thiolester Hydrolases - analysis ; Thiolester Hydrolases - genetics ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1999-04, Vol.265 (1), p.53-56</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-553cd6c47ee181bafccd29ecf77df037882f9f358bfe25a3ec9fd7d0eace9cd63</citedby><cites>FETCH-LOGICAL-c421t-553cd6c47ee181bafccd29ecf77df037882f9f358bfe25a3ec9fd7d0eace9cd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(99)00207-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1776163$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10327204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suopanki, Jaana</creatorcontrib><creatorcontrib>Tyynelä, Jaana</creatorcontrib><creatorcontrib>Baumann, Marc</creatorcontrib><creatorcontrib>Haltia, Matti</creatorcontrib><title>Palmitoyl-protein thioesterase, an enzyme implicated in neurodegeneration, is localized in neurons and is developmentally regulated in rat brain</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Palmitoyl-protein thioesterase (PPT) is an enzyme involved in cleavage of palmitate residues from acylated proteins. Mutations in PPT gene cause a severe neurodegenerative disorder, infantile neuronal ceroid-lipofuscinosis (INCL), characterized by loss of cortical neurons. In order to clarify the role of PPT and palmitoylation/depalmitoylation in the development of CNS and pathogenesis of infantile neuronal ceroid-lipofuscinosis (INCL), we studied the localization and expression of PPT in developing rats. Using immunohistochemical methods, we show for the first time that PPT is truly localized in neurons. Further, using RT-PCR and Western blotting, we show that expression of PPT in rat brain is developmentally regulated, with increasing expression during the maturation of CNS, reaching the maximum in young adulthood. The presented data support the view of PPT being essential for both development and maintenance of cortical neurons.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain development</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Immunohistochemistry</subject><subject>Infantile neuronal ceroid-lipofuscinosis</subject><subject>Nerve Degeneration</subject><subject>Neurons - chemistry</subject><subject>Palmitoyl-protein thioesterase</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RT-PCR, protein expression</subject><subject>Thiolester Hydrolases - analysis</subject><subject>Thiolester Hydrolases - genetics</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1vFCEUhonR2G31J2i4MEaTjvIxMwxXjWnqR9JEE_WasHCoGAZWYJpsf4U_Wba7au96xQXP-8I5D0LPKHlDCR3ffiWc9B2XPXkl5WtCGBFd_wCt6CRYJ6RgD9HqH3KEjkv5SQgZ6NA_RkeUcCYY6Vfo9xcdZl_TNnSbnCr4iOsPn6BUyLrAKdYRQ7zZzoD9vAne6AoWNyrCkpOFK4gNrD7FU-wLDsno4G_uILG0Cru7s3ANIW1miFWHsMUZrpbwt6514HXWPj5Bj5wOBZ4ezhP0_f3Ft_OP3eXnD5_O3112pme0dsPAjR1NLwDoRNfaGWOZBOOEsI5wMU3MSceHae2ADZqDkc4KS0AbkC3JT9DLfW8b-9fS5lWzLwZC0BHSUtQoxcAZne4FqWDTNNG-gcMeNDmVksGpTfazzltFido5U7fO1E6IklLdOlO73PPDA8t6BnsntZfUgBcHQJe2Xpd1NL7854QY6cgbdrbHoK3t2kNWxXiIBqzPYKqyyd_zkz8eHrfO</recordid><startdate>19990409</startdate><enddate>19990409</enddate><creator>Suopanki, Jaana</creator><creator>Tyynelä, Jaana</creator><creator>Baumann, Marc</creator><creator>Haltia, Matti</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19990409</creationdate><title>Palmitoyl-protein thioesterase, an enzyme implicated in neurodegeneration, is localized in neurons and is developmentally regulated in rat brain</title><author>Suopanki, Jaana ; Tyynelä, Jaana ; Baumann, Marc ; Haltia, Matti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-553cd6c47ee181bafccd29ecf77df037882f9f358bfe25a3ec9fd7d0eace9cd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain development</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Immunohistochemistry</topic><topic>Infantile neuronal ceroid-lipofuscinosis</topic><topic>Nerve Degeneration</topic><topic>Neurons - chemistry</topic><topic>Palmitoyl-protein thioesterase</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RT-PCR, protein expression</topic><topic>Thiolester Hydrolases - analysis</topic><topic>Thiolester Hydrolases - genetics</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suopanki, Jaana</creatorcontrib><creatorcontrib>Tyynelä, Jaana</creatorcontrib><creatorcontrib>Baumann, Marc</creatorcontrib><creatorcontrib>Haltia, Matti</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suopanki, Jaana</au><au>Tyynelä, Jaana</au><au>Baumann, Marc</au><au>Haltia, Matti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Palmitoyl-protein thioesterase, an enzyme implicated in neurodegeneration, is localized in neurons and is developmentally regulated in rat brain</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1999-04-09</date><risdate>1999</risdate><volume>265</volume><issue>1</issue><spage>53</spage><epage>56</epage><pages>53-56</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Palmitoyl-protein thioesterase (PPT) is an enzyme involved in cleavage of palmitate residues from acylated proteins. Mutations in PPT gene cause a severe neurodegenerative disorder, infantile neuronal ceroid-lipofuscinosis (INCL), characterized by loss of cortical neurons. In order to clarify the role of PPT and palmitoylation/depalmitoylation in the development of CNS and pathogenesis of infantile neuronal ceroid-lipofuscinosis (INCL), we studied the localization and expression of PPT in developing rats. Using immunohistochemical methods, we show for the first time that PPT is truly localized in neurons. Further, using RT-PCR and Western blotting, we show that expression of PPT in rat brain is developmentally regulated, with increasing expression during the maturation of CNS, reaching the maximum in young adulthood. The presented data support the view of PPT being essential for both development and maintenance of cortical neurons.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>10327204</pmid><doi>10.1016/S0304-3940(99)00207-4</doi><tpages>4</tpages></addata></record> |
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| ispartof | Neuroscience letters, 1999-04, Vol.265 (1), p.53-56 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biological and medical sciences Blotting, Western Brain development Development. Senescence. Regeneration. Transplantation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - physiology Immunohistochemistry Infantile neuronal ceroid-lipofuscinosis Nerve Degeneration Neurons - chemistry Palmitoyl-protein thioesterase Rats Reverse Transcriptase Polymerase Chain Reaction RT-PCR, protein expression Thiolester Hydrolases - analysis Thiolester Hydrolases - genetics Vertebrates: nervous system and sense organs |
| title | Palmitoyl-protein thioesterase, an enzyme implicated in neurodegeneration, is localized in neurons and is developmentally regulated in rat brain |
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