Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice
Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-12, Vol.377 (1), p.291-296 |
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| creator | Kondo, Yoshitaka Sasaki, Toru Sato, Yasunori Amano, Akiko Aizawa, Shingo Iwama, Mizuki Handa, Setsuko Shimada, Nobuko Fukuda, Mitsugu Akita, Masumi Lee, Jaewon Jeong, Kyu-Shik Maruyama, Naoki Ishigami, Akihito |
| description | Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals
in vitro. We verified that this property actually exists
in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC
in vivo. SMP30/GNL KO mice were given 1.5
g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(−)]. At 4 and 8 weeks, VC levels in brains from VC(−) KO mice were |
| doi_str_mv | 10.1016/j.bbrc.2008.09.132 |
| format | Article |
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in vitro. We verified that this property actually exists
in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC
in vivo. SMP30/GNL KO mice were given 1.5
g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(−)]. At 4 and 8 weeks, VC levels in brains from VC(−) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(−) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2008.09.132</identifier><identifier>PMID: 18848523</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acridines - analysis ; Acridines - metabolism ; Animals ; Antioxidants - metabolism ; Ascorbic acid ; Ascorbic Acid - genetics ; Ascorbic Acid - metabolism ; Body Weight ; Brain - metabolism ; Calcium-Binding Proteins - genetics ; Carboxylic Ester Hydrolases - genetics ; Catalase ; Chemiluminescence ; Gluconolactonase ; Intracellular Signaling Peptides and Proteins - genetics ; Luminescent Agents - analysis ; Luminescent Agents - metabolism ; Mice ; Mice, Knockout ; Oxidative stress ; ROS ; Senescence marker protein-30 ; SMP30 ; SOD ; Superoxides - analysis ; Superoxides - metabolism ; Vitamin C</subject><ispartof>Biochemical and biophysical research communications, 2008-12, Vol.377 (1), p.291-296</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-ee90132552409deefabc565192e642756667d7b64a8ae856034927a586c972413</citedby><cites>FETCH-LOGICAL-c385t-ee90132552409deefabc565192e642756667d7b64a8ae856034927a586c972413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X08019311$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18848523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kondo, Yoshitaka</creatorcontrib><creatorcontrib>Sasaki, Toru</creatorcontrib><creatorcontrib>Sato, Yasunori</creatorcontrib><creatorcontrib>Amano, Akiko</creatorcontrib><creatorcontrib>Aizawa, Shingo</creatorcontrib><creatorcontrib>Iwama, Mizuki</creatorcontrib><creatorcontrib>Handa, Setsuko</creatorcontrib><creatorcontrib>Shimada, Nobuko</creatorcontrib><creatorcontrib>Fukuda, Mitsugu</creatorcontrib><creatorcontrib>Akita, Masumi</creatorcontrib><creatorcontrib>Lee, Jaewon</creatorcontrib><creatorcontrib>Jeong, Kyu-Shik</creatorcontrib><creatorcontrib>Maruyama, Naoki</creatorcontrib><creatorcontrib>Ishigami, Akihito</creatorcontrib><title>Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals
in vitro. We verified that this property actually exists
in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC
in vivo. SMP30/GNL KO mice were given 1.5
g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(−)]. At 4 and 8 weeks, VC levels in brains from VC(−) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(−) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.</description><subject>Acridines - analysis</subject><subject>Acridines - metabolism</subject><subject>Animals</subject><subject>Antioxidants - metabolism</subject><subject>Ascorbic acid</subject><subject>Ascorbic Acid - genetics</subject><subject>Ascorbic Acid - metabolism</subject><subject>Body Weight</subject><subject>Brain - metabolism</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>Catalase</subject><subject>Chemiluminescence</subject><subject>Gluconolactonase</subject><subject>Intracellular Signaling Peptides and Proteins - genetics</subject><subject>Luminescent Agents - analysis</subject><subject>Luminescent Agents - metabolism</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Oxidative stress</subject><subject>ROS</subject><subject>Senescence marker protein-30</subject><subject>SMP30</subject><subject>SOD</subject><subject>Superoxides - analysis</subject><subject>Superoxides - metabolism</subject><subject>Vitamin C</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAUhYMoOj7-gAvJyl07N2mTNuBGBl8wPsAH7kKa3pGM02ZMWtF_b4cZcKeru7jfORw-Qo4ZpAyYHM_Tqgo25QBlCiplGd8iIwYKEs4g3yYjAJAJV-x1j-zHOAdgLJdql-yxssxLwbMReX1xnWlcSye0xuUCO-db6lob0ESMNPZLDP7L1UjfsMVgNn9aBePaSP2MPt4-ZDC-upvS99bbd993tHEWD8nOzCwiHm3uAXm-vHiaXCfT-6ubyfk0sVkpugRRwTBcCJ6DqhFnprJCCqY4ypwXQkpZ1EUlc1MaLIWELFe8MKKUVhU8Z9kBOV33LoP_6DF2unHR4mJhWvR91FIVYmXoX5CpjEslYAD5GrTBxxhwppfBNSZ8awZ6JV7P9Uq8XtVqUHrYP4RONu191WD9G9mYHoCzNYCDjE-HQUfrsLVYu4C207V3f_X_ADAMknQ</recordid><startdate>20081205</startdate><enddate>20081205</enddate><creator>Kondo, Yoshitaka</creator><creator>Sasaki, Toru</creator><creator>Sato, Yasunori</creator><creator>Amano, Akiko</creator><creator>Aizawa, Shingo</creator><creator>Iwama, Mizuki</creator><creator>Handa, Setsuko</creator><creator>Shimada, Nobuko</creator><creator>Fukuda, Mitsugu</creator><creator>Akita, Masumi</creator><creator>Lee, Jaewon</creator><creator>Jeong, Kyu-Shik</creator><creator>Maruyama, Naoki</creator><creator>Ishigami, Akihito</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20081205</creationdate><title>Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice</title><author>Kondo, Yoshitaka ; Sasaki, Toru ; Sato, Yasunori ; Amano, Akiko ; Aizawa, Shingo ; Iwama, Mizuki ; Handa, Setsuko ; Shimada, Nobuko ; Fukuda, Mitsugu ; Akita, Masumi ; Lee, Jaewon ; Jeong, Kyu-Shik ; Maruyama, Naoki ; Ishigami, Akihito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-ee90132552409deefabc565192e642756667d7b64a8ae856034927a586c972413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acridines - analysis</topic><topic>Acridines - metabolism</topic><topic>Animals</topic><topic>Antioxidants - metabolism</topic><topic>Ascorbic acid</topic><topic>Ascorbic Acid - genetics</topic><topic>Ascorbic Acid - metabolism</topic><topic>Body Weight</topic><topic>Brain - metabolism</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>Catalase</topic><topic>Chemiluminescence</topic><topic>Gluconolactonase</topic><topic>Intracellular Signaling Peptides and Proteins - genetics</topic><topic>Luminescent Agents - analysis</topic><topic>Luminescent Agents - metabolism</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Oxidative stress</topic><topic>ROS</topic><topic>Senescence marker protein-30</topic><topic>SMP30</topic><topic>SOD</topic><topic>Superoxides - analysis</topic><topic>Superoxides - metabolism</topic><topic>Vitamin C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kondo, Yoshitaka</creatorcontrib><creatorcontrib>Sasaki, Toru</creatorcontrib><creatorcontrib>Sato, Yasunori</creatorcontrib><creatorcontrib>Amano, Akiko</creatorcontrib><creatorcontrib>Aizawa, Shingo</creatorcontrib><creatorcontrib>Iwama, Mizuki</creatorcontrib><creatorcontrib>Handa, Setsuko</creatorcontrib><creatorcontrib>Shimada, Nobuko</creatorcontrib><creatorcontrib>Fukuda, Mitsugu</creatorcontrib><creatorcontrib>Akita, Masumi</creatorcontrib><creatorcontrib>Lee, Jaewon</creatorcontrib><creatorcontrib>Jeong, Kyu-Shik</creatorcontrib><creatorcontrib>Maruyama, Naoki</creatorcontrib><creatorcontrib>Ishigami, Akihito</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kondo, Yoshitaka</au><au>Sasaki, Toru</au><au>Sato, Yasunori</au><au>Amano, Akiko</au><au>Aizawa, Shingo</au><au>Iwama, Mizuki</au><au>Handa, Setsuko</au><au>Shimada, Nobuko</au><au>Fukuda, Mitsugu</au><au>Akita, Masumi</au><au>Lee, Jaewon</au><au>Jeong, Kyu-Shik</au><au>Maruyama, Naoki</au><au>Ishigami, Akihito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-12-05</date><risdate>2008</risdate><volume>377</volume><issue>1</issue><spage>291</spage><epage>296</epage><pages>291-296</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals
in vitro. We verified that this property actually exists
in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC
in vivo. SMP30/GNL KO mice were given 1.5
g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(−)]. At 4 and 8 weeks, VC levels in brains from VC(−) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(−) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18848523</pmid><doi>10.1016/j.bbrc.2008.09.132</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2008-12, Vol.377 (1), p.291-296 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Collection |
| subjects | Acridines - analysis Acridines - metabolism Animals Antioxidants - metabolism Ascorbic acid Ascorbic Acid - genetics Ascorbic Acid - metabolism Body Weight Brain - metabolism Calcium-Binding Proteins - genetics Carboxylic Ester Hydrolases - genetics Catalase Chemiluminescence Gluconolactonase Intracellular Signaling Peptides and Proteins - genetics Luminescent Agents - analysis Luminescent Agents - metabolism Mice Mice, Knockout Oxidative stress ROS Senescence marker protein-30 SMP30 SOD Superoxides - analysis Superoxides - metabolism Vitamin C |
| title | Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice |
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