Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae
Pyruvate-decarboxylase (Pdc)-negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose-containing complex media, but not on defined glucose-containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cere...
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| Veröffentlicht in: | FEMS microbiology letters 1999-05, Vol.174 (1), p.73-79 |
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| container_title | FEMS microbiology letters |
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| creator | Flikweert, M.T Swaaf, M. de Dijken, J.P. van Pronk, J.T |
| description | Pyruvate-decarboxylase (Pdc)-negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose-containing complex media, but not on defined glucose-containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cerevisiae does not exhibit prolonged growth on glucose. Pdc- strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA. |
| doi_str_mv | 10.1111/j.1574-6968.1999.tb13551.x |
| format | Article |
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By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cerevisiae does not exhibit prolonged growth on glucose. Pdc- strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1999.tb13551.x</identifier><identifier>PMID: 10234824</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetates - metabolism ; Acetic acid ; Acetyl Coenzyme A - metabolism ; Biological and medical sciences ; Chemostat cultivation ; Complex media ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Glucose ; Glucose - metabolism ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Lipids ; Lysine ; Metabolic compartmentation ; Microbiology ; Models, Theoretical ; Mycology ; Nutrient requirements ; plant biochemistry ; plant physiology ; Pyruvate decarboxylase ; Pyruvate decarboxylase (EC 4.1.1.1) ; Pyruvate Decarboxylase - deficiency ; Pyruvic acid ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - growth & development ; Synthesis ; Yeast</subject><ispartof>FEMS microbiology letters, 1999-05, Vol.174 (1), p.73-79</ispartof><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1999</rights><rights>1999 INIST-CNRS</rights><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 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By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cerevisiae does not exhibit prolonged growth on glucose. Pdc- strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA.</description><subject>Acetates - metabolism</subject><subject>Acetic acid</subject><subject>Acetyl Coenzyme A - metabolism</subject><subject>Biological and medical sciences</subject><subject>Chemostat cultivation</subject><subject>Complex media</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Lipids</subject><subject>Lysine</subject><subject>Metabolic compartmentation</subject><subject>Microbiology</subject><subject>Models, Theoretical</subject><subject>Mycology</subject><subject>Nutrient requirements</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>Pyruvate decarboxylase</subject><subject>Pyruvate decarboxylase (EC 4.1.1.1)</subject><subject>Pyruvate Decarboxylase - deficiency</subject><subject>Pyruvic acid</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Synthesis</subject><subject>Yeast</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkc1u1DAURi0EokPhFSACxC7Bf7HjSixQRQvSoC5K15bHvm4zSuKpnUwnb0-ijAAhEMIbL3zO9Xf1IfSa4IJM5_22IKXkuVCiKohSqug3hJUlKQ6P0OrH02O0wkxWOcFKnqBnKW0xxpxi8RSdEEwZryhfoavLGB76uyzC_VBHaKHrUxZ8thvjsDc95A6siZtwGBuTIO_g1vT1HrJrY-2diaEdLaTMQoR9nWoDz9ETb5oEL473Kbq5-PTt_HO-vrr8cv5xnVteKpYbrriXjFuqCFeV985Z5zyW4KTg4HxVUiOwIURIBYrjikmyMQ44F9wZwU7Ru2XuLob7AVKv2zpZaBrTQRiSFkoyWTL5T5BIyjHl88Q3v4HbMMRuWkJTRogsKVPzuLOFsjGkFMHrXaxbE0dNsJ7b0Vs9V6DnCvTcjj62ow-T_PL4xbBpwf2iLnVMwNsjYJI1jY-ms3X6yVUYV2IO8WHBHuoGxv9IoC--riWb_HLxw7D7i53_Of-rxfMmaHMbp2g31xQThqmimEvCvgOI2MWG</recordid><startdate>199905</startdate><enddate>199905</enddate><creator>Flikweert, M.T</creator><creator>Swaaf, M. de</creator><creator>Dijken, J.P. van</creator><creator>Pronk, J.T</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199905</creationdate><title>Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae</title><author>Flikweert, M.T ; Swaaf, M. de ; Dijken, J.P. van ; Pronk, J.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4593-a494f734c291498ffddcddf07ed764edf852a60a11679e9408371bade4464da63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acetates - metabolism</topic><topic>Acetic acid</topic><topic>Acetyl Coenzyme A - metabolism</topic><topic>Biological and medical sciences</topic><topic>Chemostat cultivation</topic><topic>Complex media</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Lipids</topic><topic>Lysine</topic><topic>Metabolic compartmentation</topic><topic>Microbiology</topic><topic>Models, Theoretical</topic><topic>Mycology</topic><topic>Nutrient requirements</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>Pyruvate decarboxylase</topic><topic>Pyruvate decarboxylase (EC 4.1.1.1)</topic><topic>Pyruvate Decarboxylase - deficiency</topic><topic>Pyruvic acid</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Synthesis</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flikweert, M.T</creatorcontrib><creatorcontrib>Swaaf, M. de</creatorcontrib><creatorcontrib>Dijken, J.P. van</creatorcontrib><creatorcontrib>Pronk, J.T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flikweert, M.T</au><au>Swaaf, M. de</au><au>Dijken, J.P. van</au><au>Pronk, J.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1999-05</date><risdate>1999</risdate><volume>174</volume><issue>1</issue><spage>73</spage><epage>79</epage><pages>73-79</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Pyruvate-decarboxylase (Pdc)-negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose-containing complex media, but not on defined glucose-containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc- S. cerevisiae does not exhibit prolonged growth on glucose. Pdc- strains do grow in carbon-limited cultures on defined media containing glucose-acetate mixtures. The acetate requirement for glucose-limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl-CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl-CoA.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10234824</pmid><doi>10.1111/j.1574-6968.1999.tb13551.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEMS microbiology letters, 1999-05, Vol.174 (1), p.73-79 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library; AUTh Library subscriptions: Oxford University Press; Alma/SFX Local Collection |
| subjects | Acetates - metabolism Acetic acid Acetyl Coenzyme A - metabolism Biological and medical sciences Chemostat cultivation Complex media Culture Media Fundamental and applied biological sciences. Psychology Glucose Glucose - metabolism Growth, nutrition, metabolism, transports, enzymes. Molecular biology Lipids Lysine Metabolic compartmentation Microbiology Models, Theoretical Mycology Nutrient requirements plant biochemistry plant physiology Pyruvate decarboxylase Pyruvate decarboxylase (EC 4.1.1.1) Pyruvate Decarboxylase - deficiency Pyruvic acid Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - growth & development Synthesis Yeast |
| title | Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae |
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