Prospective comparison of the Gen-probe PACE 2 Assay and the Abbott Ligase Chain Reaction for the direct detection of Chlamydia trachomatis in a low prevalence population
* Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono, ME 04469 Affiliated Laboratory Inc., 925 Union Street, Suite 4, Bangor, ME 04401, USA 1 Corresponding author: Dr K. M. Doing. Received May 5, 1998 Revision received August 26, 1998. Accepted August 26, 1998...
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| Veröffentlicht in: | Journal of medical microbiology 1999-05, Vol.48 (5), p.507-510 |
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| container_title | Journal of medical microbiology |
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| creator | DOING, KIRK M CURTIS, KRISTOPHER LONG, JENNIFER W VOLOCK, MARGARET L |
| description | * Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono, ME 04469
Affiliated Laboratory Inc., 925 Union Street, Suite 4, Bangor, ME 04401, USA
1 Corresponding author: Dr K. M. Doing.
Received May 5, 1998
Revision received August 26, 1998.
Accepted August 26, 1998
In a prospective study, the Gen-Probe PACE 2 (GP) assay was compared with Abbott Laboratories ligase chain reaction (LCR) assay for the detection of Chlamydia trachomatis . A total of 493 female patients consented to collection of two cervical samples; a first-void urine (FVU) sample was collected also from 446 of the participants. Cervical samples were tested by both GP and LCR; 16 samples (3.1%) tested positive by both methods and no discrepant results were observed. All but one of the FVU samples collected from patients with a positive cervical sample was positive for C. trachomatis by LCR. The stability of FVU samples over time in the LCR test was also evaluated and proved to be significantly longer than the 4 days stated by the manufacturer. While LCR proved to be highly sensitive in detecting chlamydial infection in FVU samples, no difference was noted between LCR and GP in the detection of cervical C. trachomatis infection in this study population. |
| doi_str_mv | 10.1099/00222615-48-5-507 |
| format | Article |
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Affiliated Laboratory Inc., 925 Union Street, Suite 4, Bangor, ME 04401, USA
1 Corresponding author: Dr K. M. Doing.
Received May 5, 1998
Revision received August 26, 1998.
Accepted August 26, 1998
In a prospective study, the Gen-Probe PACE 2 (GP) assay was compared with Abbott Laboratories ligase chain reaction (LCR) assay for the detection of Chlamydia trachomatis . A total of 493 female patients consented to collection of two cervical samples; a first-void urine (FVU) sample was collected also from 446 of the participants. Cervical samples were tested by both GP and LCR; 16 samples (3.1%) tested positive by both methods and no discrepant results were observed. All but one of the FVU samples collected from patients with a positive cervical sample was positive for C. trachomatis by LCR. The stability of FVU samples over time in the LCR test was also evaluated and proved to be significantly longer than the 4 days stated by the manufacturer. While LCR proved to be highly sensitive in detecting chlamydial infection in FVU samples, no difference was noted between LCR and GP in the detection of cervical C. trachomatis infection in this study population.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/00222615-48-5-507</identifier><identifier>PMID: 10229549</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Adolescent ; Adult ; Bacterial diseases ; Bacterial diseases of the genital system ; Biological and medical sciences ; Cervix Uteri - microbiology ; Chlamydia Infections - diagnosis ; Chlamydia Infections - urine ; Chlamydia trachomatis ; Chlamydia trachomatis - genetics ; Chlamydia trachomatis - isolation & purification ; DNA, Bacterial - isolation & purification ; Female ; Human bacterial diseases ; Humans ; Infectious diseases ; Medical sciences ; Middle Aged ; Nucleic Acid Amplification Techniques ; Nucleic Acid Hybridization - methods ; Prospective Studies ; Reagent Kits, Diagnostic - economics</subject><ispartof>Journal of medical microbiology, 1999-05, Vol.48 (5), p.507-510</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-fc7d7798ab4e26b73fa9e992559c7a6c20026b427891b9eb79f4ec5f1ee62fa03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1794701$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10229549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DOING, KIRK M</creatorcontrib><creatorcontrib>CURTIS, KRISTOPHER</creatorcontrib><creatorcontrib>LONG, JENNIFER W</creatorcontrib><creatorcontrib>VOLOCK, MARGARET L</creatorcontrib><title>Prospective comparison of the Gen-probe PACE 2 Assay and the Abbott Ligase Chain Reaction for the direct detection of Chlamydia trachomatis in a low prevalence population</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>* Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono, ME 04469
Affiliated Laboratory Inc., 925 Union Street, Suite 4, Bangor, ME 04401, USA
1 Corresponding author: Dr K. M. Doing.
Received May 5, 1998
Revision received August 26, 1998.
Accepted August 26, 1998
In a prospective study, the Gen-Probe PACE 2 (GP) assay was compared with Abbott Laboratories ligase chain reaction (LCR) assay for the detection of Chlamydia trachomatis . A total of 493 female patients consented to collection of two cervical samples; a first-void urine (FVU) sample was collected also from 446 of the participants. Cervical samples were tested by both GP and LCR; 16 samples (3.1%) tested positive by both methods and no discrepant results were observed. All but one of the FVU samples collected from patients with a positive cervical sample was positive for C. trachomatis by LCR. The stability of FVU samples over time in the LCR test was also evaluated and proved to be significantly longer than the 4 days stated by the manufacturer. 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CURTIS, KRISTOPHER ; LONG, JENNIFER W ; VOLOCK, MARGARET L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-fc7d7798ab4e26b73fa9e992559c7a6c20026b427891b9eb79f4ec5f1ee62fa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the genital system</topic><topic>Biological and medical sciences</topic><topic>Cervix Uteri - microbiology</topic><topic>Chlamydia Infections - diagnosis</topic><topic>Chlamydia Infections - urine</topic><topic>Chlamydia trachomatis</topic><topic>Chlamydia trachomatis - genetics</topic><topic>Chlamydia trachomatis - isolation & purification</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Female</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Prospective Studies</topic><topic>Reagent Kits, Diagnostic - economics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DOING, KIRK M</creatorcontrib><creatorcontrib>CURTIS, KRISTOPHER</creatorcontrib><creatorcontrib>LONG, JENNIFER W</creatorcontrib><creatorcontrib>VOLOCK, MARGARET L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DOING, KIRK M</au><au>CURTIS, KRISTOPHER</au><au>LONG, JENNIFER W</au><au>VOLOCK, MARGARET L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prospective comparison of the Gen-probe PACE 2 Assay and the Abbott Ligase Chain Reaction for the direct detection of Chlamydia trachomatis in a low prevalence population</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>48</volume><issue>5</issue><spage>507</spage><epage>510</epage><pages>507-510</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>* Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono, ME 04469
Affiliated Laboratory Inc., 925 Union Street, Suite 4, Bangor, ME 04401, USA
1 Corresponding author: Dr K. M. Doing.
Received May 5, 1998
Revision received August 26, 1998.
Accepted August 26, 1998
In a prospective study, the Gen-Probe PACE 2 (GP) assay was compared with Abbott Laboratories ligase chain reaction (LCR) assay for the detection of Chlamydia trachomatis . A total of 493 female patients consented to collection of two cervical samples; a first-void urine (FVU) sample was collected also from 446 of the participants. Cervical samples were tested by both GP and LCR; 16 samples (3.1%) tested positive by both methods and no discrepant results were observed. All but one of the FVU samples collected from patients with a positive cervical sample was positive for C. trachomatis by LCR. The stability of FVU samples over time in the LCR test was also evaluated and proved to be significantly longer than the 4 days stated by the manufacturer. While LCR proved to be highly sensitive in detecting chlamydial infection in FVU samples, no difference was noted between LCR and GP in the detection of cervical C. trachomatis infection in this study population.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>10229549</pmid><doi>10.1099/00222615-48-5-507</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adolescent Adult Bacterial diseases Bacterial diseases of the genital system Biological and medical sciences Cervix Uteri - microbiology Chlamydia Infections - diagnosis Chlamydia Infections - urine Chlamydia trachomatis Chlamydia trachomatis - genetics Chlamydia trachomatis - isolation & purification DNA, Bacterial - isolation & purification Female Human bacterial diseases Humans Infectious diseases Medical sciences Middle Aged Nucleic Acid Amplification Techniques Nucleic Acid Hybridization - methods Prospective Studies Reagent Kits, Diagnostic - economics |
| title | Prospective comparison of the Gen-probe PACE 2 Assay and the Abbott Ligase Chain Reaction for the direct detection of Chlamydia trachomatis in a low prevalence population |
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