Candida albicans Mannan Extract—Protein Conjugates Induce a Protective Immune Response against Experimental Candidiasis
Candida albicans mannan extracts encapsulated in liposomes were previously used to stimulate mice to produce antibodies protective against candidiasis. In the present study, mannan-protein conjugates without liposomes were tested as vaccine candidates. Mannan extracts were coupled to bovine serum al...
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| Veröffentlicht in: | The Journal of infectious diseases 1999-06, Vol.179 (6), p.1477-1484 |
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| container_title | The Journal of infectious diseases |
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| creator | Han, Yongmoon Ulrich, Melinda A. Cutler, Jim E. |
| description | Candida albicans mannan extracts encapsulated in liposomes were previously used to stimulate mice to produce antibodies protective against candidiasis. In the present study, mannan-protein conjugates without liposomes were tested as vaccine candidates. Mannan extracts were coupled to bovine serum albumin, and isolated conjugates consisted of carbohydrate and protein at a ratio of 0.7–1.0. Vaccination of mice with the conjugate and an adjuvant yielded antiserum that contained Candida agglutinins. Vaccinated mice challenged with yeast cells had a mean survival time of 56 days, compared with |
| doi_str_mv | 10.1086/314779 |
| format | Article |
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In the present study, mannan-protein conjugates without liposomes were tested as vaccine candidates. Mannan extracts were coupled to bovine serum albumin, and isolated conjugates consisted of carbohydrate and protein at a ratio of 0.7–1.0. Vaccination of mice with the conjugate and an adjuvant yielded antiserum that contained Candida agglutinins. Vaccinated mice challenged with yeast cells had a mean survival time of 56 days, compared with <13 days for control groups. The antiserum protected naive animals against disseminated disease. Naive mice given the anti-serum intravaginally developed 79% fewer fungal colony-forming units, compared with control groups. The serum-protective factor was stable at 56°C and was removed by adsorption with yeast cells. It is concluded that the conjugate vaccine can induce protective antibody responses against experimental disseminated candidiasis and Candida vaginal infection.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/314779</identifier><identifier>PMID: 10228070</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: University Chicago Press</publisher><subject>Animals ; Antibodies ; Antibodies, Fungal - blood ; Antibody Specificity ; Antiserum ; Biological and medical sciences ; Candida ; Candida albicans ; Candida albicans - immunology ; Candidiasis ; Candidiasis - immunology ; Candidiasis - prevention & control ; Drug Carriers ; Drug Compounding ; Experimental mycoses and models ; Female ; Fungal Vaccines - immunology ; Fungal Vaccines - therapeutic use ; Immunization, Passive ; Immunoconjugates - immunology ; Immunoconjugates - therapeutic use ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Infections ; Infectious diseases ; Kidneys ; Liposomes ; Major Articles ; Mannan ; Mannans ; Mannans - immunology ; Mannans - therapeutic use ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mycoses ; Vaccination ; Yeasts</subject><ispartof>The Journal of infectious diseases, 1999-06, Vol.179 (6), p.1477-1484</ispartof><rights>Copyright 1999 Triangle Pharmaceuticals</rights><rights>1999 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Jun 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-1b61a3d406af595311e86d2bbd1230ec1a07f2e924259797ff1bdfcb6b17a3dc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30117421$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30117421$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1802083$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10228070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Yongmoon</creatorcontrib><creatorcontrib>Ulrich, Melinda A.</creatorcontrib><creatorcontrib>Cutler, Jim E.</creatorcontrib><title>Candida albicans Mannan Extract—Protein Conjugates Induce a Protective Immune Response against Experimental Candidiasis</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>Candida albicans mannan extracts encapsulated in liposomes were previously used to stimulate mice to produce antibodies protective against candidiasis. In the present study, mannan-protein conjugates without liposomes were tested as vaccine candidates. Mannan extracts were coupled to bovine serum albumin, and isolated conjugates consisted of carbohydrate and protein at a ratio of 0.7–1.0. Vaccination of mice with the conjugate and an adjuvant yielded antiserum that contained Candida agglutinins. Vaccinated mice challenged with yeast cells had a mean survival time of 56 days, compared with <13 days for control groups. The antiserum protected naive animals against disseminated disease. Naive mice given the anti-serum intravaginally developed 79% fewer fungal colony-forming units, compared with control groups. The serum-protective factor was stable at 56°C and was removed by adsorption with yeast cells. It is concluded that the conjugate vaccine can induce protective antibody responses against experimental disseminated candidiasis and Candida vaginal infection.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Fungal - blood</subject><subject>Antibody Specificity</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Candida</subject><subject>Candida albicans</subject><subject>Candida albicans - immunology</subject><subject>Candidiasis</subject><subject>Candidiasis - immunology</subject><subject>Candidiasis - prevention & control</subject><subject>Drug Carriers</subject><subject>Drug Compounding</subject><subject>Experimental mycoses and models</subject><subject>Female</subject><subject>Fungal Vaccines - immunology</subject><subject>Fungal Vaccines - therapeutic use</subject><subject>Immunization, Passive</subject><subject>Immunoconjugates - immunology</subject><subject>Immunoconjugates - therapeutic use</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - blood</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Kidneys</subject><subject>Liposomes</subject><subject>Major Articles</subject><subject>Mannan</subject><subject>Mannans</subject><subject>Mannans - immunology</subject><subject>Mannans - therapeutic use</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mycoses</subject><subject>Vaccination</subject><subject>Yeasts</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURiMEokOBNwBZCLEL-NqJHS9R1KGDikD8CbGxbhyn8pBxBttB7Y6H4Al5EgwZtYgNKy_Oud-V71cU94E-BdqIZxwqKdWNYgU1l6UQwG8WK0oZK6FR6qi4E-OWUlpxIW8XR5BBQyVdFZct-t71SHDsnEEfySv0Hj05uUgBTfr5_cebMCXrPGknv53PMdlINr6fjSVI_jCT3DdLNrvd7C15a-N-8jHDc3Q-phy0t8HtrE84kmWbw-ji3eLWgGO09w7vcfFhffK-PS3PXr_YtM_PSlMxlkroBCDvKypwqFXNAWwjetZ1PTBOrQGkcmBWsYrVSio5DND1g-lEBzLPGX5cPFly92H6OtuY9M5FY8cRvZ3mqIWSHBpZ_1eEhgOrQGXx0T_idpqDz5_QjHFFBefVdZoJU4zBDnqfr4DhUgPVvyvTS2VZfHhIm7ud7f_Slo6y8PggYDQ4DgG9cfHaayijDc_ag0XbxjSFK8wpgKwYZF4u3MVkL644hi9aSC5rffrps_7YvnzH102l1_wXYrq28w</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Han, Yongmoon</creator><creator>Ulrich, Melinda A.</creator><creator>Cutler, Jim E.</creator><general>University Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Candida albicans Mannan Extract—Protein Conjugates Induce a Protective Immune Response against Experimental Candidiasis</title><author>Han, Yongmoon ; Ulrich, Melinda A. ; Cutler, Jim E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-1b61a3d406af595311e86d2bbd1230ec1a07f2e924259797ff1bdfcb6b17a3dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Fungal - blood</topic><topic>Antibody Specificity</topic><topic>Antiserum</topic><topic>Biological and medical sciences</topic><topic>Candida</topic><topic>Candida albicans</topic><topic>Candida albicans - immunology</topic><topic>Candidiasis</topic><topic>Candidiasis - immunology</topic><topic>Candidiasis - prevention & control</topic><topic>Drug Carriers</topic><topic>Drug Compounding</topic><topic>Experimental mycoses and models</topic><topic>Female</topic><topic>Fungal Vaccines - immunology</topic><topic>Fungal Vaccines - therapeutic use</topic><topic>Immunization, Passive</topic><topic>Immunoconjugates - immunology</topic><topic>Immunoconjugates - therapeutic use</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - blood</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Kidneys</topic><topic>Liposomes</topic><topic>Major Articles</topic><topic>Mannan</topic><topic>Mannans</topic><topic>Mannans - immunology</topic><topic>Mannans - therapeutic use</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mycoses</topic><topic>Vaccination</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Yongmoon</creatorcontrib><creatorcontrib>Ulrich, Melinda A.</creatorcontrib><creatorcontrib>Cutler, Jim E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Yongmoon</au><au>Ulrich, Melinda A.</au><au>Cutler, Jim E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Candida albicans Mannan Extract—Protein Conjugates Induce a Protective Immune Response against Experimental Candidiasis</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>179</volume><issue>6</issue><spage>1477</spage><epage>1484</epage><pages>1477-1484</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Candida albicans mannan extracts encapsulated in liposomes were previously used to stimulate mice to produce antibodies protective against candidiasis. In the present study, mannan-protein conjugates without liposomes were tested as vaccine candidates. Mannan extracts were coupled to bovine serum albumin, and isolated conjugates consisted of carbohydrate and protein at a ratio of 0.7–1.0. Vaccination of mice with the conjugate and an adjuvant yielded antiserum that contained Candida agglutinins. Vaccinated mice challenged with yeast cells had a mean survival time of 56 days, compared with <13 days for control groups. The antiserum protected naive animals against disseminated disease. Naive mice given the anti-serum intravaginally developed 79% fewer fungal colony-forming units, compared with control groups. The serum-protective factor was stable at 56°C and was removed by adsorption with yeast cells. It is concluded that the conjugate vaccine can induce protective antibody responses against experimental disseminated candidiasis and Candida vaginal infection.</abstract><cop>Chicago, IL</cop><pub>University Chicago Press</pub><pmid>10228070</pmid><doi>10.1086/314779</doi><tpages>8</tpages></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Animals Antibodies Antibodies, Fungal - blood Antibody Specificity Antiserum Biological and medical sciences Candida Candida albicans Candida albicans - immunology Candidiasis Candidiasis - immunology Candidiasis - prevention & control Drug Carriers Drug Compounding Experimental mycoses and models Female Fungal Vaccines - immunology Fungal Vaccines - therapeutic use Immunization, Passive Immunoconjugates - immunology Immunoconjugates - therapeutic use Immunoglobulin G - blood Immunoglobulin M - blood Infections Infectious diseases Kidneys Liposomes Major Articles Mannan Mannans Mannans - immunology Mannans - therapeutic use Medical sciences Mice Mice, Inbred BALB C Mycoses Vaccination Yeasts |
| title | Candida albicans Mannan Extract—Protein Conjugates Induce a Protective Immune Response against Experimental Candidiasis |
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