K-ras codon 12 and p53 mutations in biopsy specimens and bile from biliary tract cancers

To evaluate whether it is useful for diagnosis to detect K‐ras and p53 mutations in biopsy specimens and bile of biliary tract lesions, 12 cholangiocarcinomas (CC), eight cases of cholangitis, seven gallbladder carcinomas (GBC), seven gallbladder cholesterol polyps, four cases of adenomyomatosis of...

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Veröffentlicht in:Pathology international 1999-01, Vol.49 (1), p.30-37
Hauptverfasser: Itoi, Takao, Takei, Kazuo, Shinohara, Yasushi, Takeda, Kazuya, Nakamura, Kazuto, Horibe, Toshiya, Sanada, Atsushi, Ohno, Hiroyuki, Matsubayashi, Hiroyuki, Saito, Toshihiko, Watanabe, Hidenobu
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container_issue 1
container_start_page 30
container_title Pathology international
container_volume 49
creator Itoi, Takao
Takei, Kazuo
Shinohara, Yasushi
Takeda, Kazuya
Nakamura, Kazuto
Horibe, Toshiya
Sanada, Atsushi
Ohno, Hiroyuki
Matsubayashi, Hiroyuki
Saito, Toshihiko
Watanabe, Hidenobu
description To evaluate whether it is useful for diagnosis to detect K‐ras and p53 mutations in biopsy specimens and bile of biliary tract lesions, 12 cholangiocarcinomas (CC), eight cases of cholangitis, seven gallbladder carcinomas (GBC), seven gallbladder cholesterol polyps, four cases of adenomyomatosis of the gallbladder and five cases of cholecystitis were examined. K‐ras and p53 mutations in bile were detected by a two‐step polymerase chain reaction (PCR) and nested PCR–single‐strand conformation polymorphism (SSCP) analysis. In addition, p53 protein expression in biopsy specimens from CC were examined by immunostaining. K‐ras mutations at codon 12 were detected in 50% of CC and 57.1% of GBC in both biopsy specimens and bile. The incidence of p53 mutations was 33.3% in CC and 42.9% in GBC. p53 protein overexpression was observed in 60% CC biopsy specimens. In contrast, K‐ras and p53 abnormalities were not detected in any non‐neoplastic biliary tract lesion. K‐ras and p53 mutations in biliary tract cancers showed the same mutation patterns in spite of differences in the collection methods used between bile and biopsy specimens or surgically resected tissue. Genetic analysis of K‐ras and p53 mutations in biopsy specimens and bile may be useful for the diagnosis of biliary tract cancers, although it may be effectively limited to patients with advanced disease.
doi_str_mv 10.1046/j.1440-1827.1999.00821.x
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K‐ras and p53 mutations in bile were detected by a two‐step polymerase chain reaction (PCR) and nested PCR–single‐strand conformation polymorphism (SSCP) analysis. In addition, p53 protein expression in biopsy specimens from CC were examined by immunostaining. K‐ras mutations at codon 12 were detected in 50% of CC and 57.1% of GBC in both biopsy specimens and bile. The incidence of p53 mutations was 33.3% in CC and 42.9% in GBC. p53 protein overexpression was observed in 60% CC biopsy specimens. In contrast, K‐ras and p53 abnormalities were not detected in any non‐neoplastic biliary tract lesion. K‐ras and p53 mutations in biliary tract cancers showed the same mutation patterns in spite of differences in the collection methods used between bile and biopsy specimens or surgically resected tissue. 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K‐ras and p53 mutations in bile were detected by a two‐step polymerase chain reaction (PCR) and nested PCR–single‐strand conformation polymorphism (SSCP) analysis. In addition, p53 protein expression in biopsy specimens from CC were examined by immunostaining. K‐ras mutations at codon 12 were detected in 50% of CC and 57.1% of GBC in both biopsy specimens and bile. The incidence of p53 mutations was 33.3% in CC and 42.9% in GBC. p53 protein overexpression was observed in 60% CC biopsy specimens. In contrast, K‐ras and p53 abnormalities were not detected in any non‐neoplastic biliary tract lesion. K‐ras and p53 mutations in biliary tract cancers showed the same mutation patterns in spite of differences in the collection methods used between bile and biopsy specimens or surgically resected tissue. 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Takei, Kazuo ; Shinohara, Yasushi ; Takeda, Kazuya ; Nakamura, Kazuto ; Horibe, Toshiya ; Sanada, Atsushi ; Ohno, Hiroyuki ; Matsubayashi, Hiroyuki ; Saito, Toshihiko ; Watanabe, Hidenobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4021-7da3646dc385fac881fce01bf07d41dd2c077e65d02812b2e74f28b749f8e8083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Base Sequence</topic><topic>bile</topic><topic>Bile - chemistry</topic><topic>Bile - cytology</topic><topic>biliary cancer</topic><topic>Biliary Tract - chemistry</topic><topic>Biliary Tract - pathology</topic><topic>Biliary Tract Neoplasms - metabolism</topic><topic>Biliary Tract Neoplasms - pathology</topic><topic>Biopsy</topic><topic>Codon</topic><topic>DNA, Neoplasm - genetics</topic><topic>Genes, p53 - genetics</topic><topic>Genes, ras - genetics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>K-ras mutation</topic><topic>Mutation</topic><topic>p53 mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Tumor Suppressor Protein p53 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itoi, Takao</creatorcontrib><creatorcontrib>Takei, Kazuo</creatorcontrib><creatorcontrib>Shinohara, Yasushi</creatorcontrib><creatorcontrib>Takeda, Kazuya</creatorcontrib><creatorcontrib>Nakamura, Kazuto</creatorcontrib><creatorcontrib>Horibe, Toshiya</creatorcontrib><creatorcontrib>Sanada, Atsushi</creatorcontrib><creatorcontrib>Ohno, Hiroyuki</creatorcontrib><creatorcontrib>Matsubayashi, Hiroyuki</creatorcontrib><creatorcontrib>Saito, Toshihiko</creatorcontrib><creatorcontrib>Watanabe, Hidenobu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itoi, Takao</au><au>Takei, Kazuo</au><au>Shinohara, Yasushi</au><au>Takeda, Kazuya</au><au>Nakamura, Kazuto</au><au>Horibe, Toshiya</au><au>Sanada, Atsushi</au><au>Ohno, Hiroyuki</au><au>Matsubayashi, Hiroyuki</au><au>Saito, Toshihiko</au><au>Watanabe, Hidenobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>K-ras codon 12 and p53 mutations in biopsy specimens and bile from biliary tract cancers</atitle><jtitle>Pathology international</jtitle><addtitle>Pathol Int</addtitle><date>1999-01</date><risdate>1999</risdate><volume>49</volume><issue>1</issue><spage>30</spage><epage>37</epage><pages>30-37</pages><issn>1320-5463</issn><eissn>1440-1827</eissn><abstract>To evaluate whether it is useful for diagnosis to detect K‐ras and p53 mutations in biopsy specimens and bile of biliary tract lesions, 12 cholangiocarcinomas (CC), eight cases of cholangitis, seven gallbladder carcinomas (GBC), seven gallbladder cholesterol polyps, four cases of adenomyomatosis of the gallbladder and five cases of cholecystitis were examined. K‐ras and p53 mutations in bile were detected by a two‐step polymerase chain reaction (PCR) and nested PCR–single‐strand conformation polymorphism (SSCP) analysis. In addition, p53 protein expression in biopsy specimens from CC were examined by immunostaining. K‐ras mutations at codon 12 were detected in 50% of CC and 57.1% of GBC in both biopsy specimens and bile. The incidence of p53 mutations was 33.3% in CC and 42.9% in GBC. p53 protein overexpression was observed in 60% CC biopsy specimens. In contrast, K‐ras and p53 abnormalities were not detected in any non‐neoplastic biliary tract lesion. K‐ras and p53 mutations in biliary tract cancers showed the same mutation patterns in spite of differences in the collection methods used between bile and biopsy specimens or surgically resected tissue. Genetic analysis of K‐ras and p53 mutations in biopsy specimens and bile may be useful for the diagnosis of biliary tract cancers, although it may be effectively limited to patients with advanced disease.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><pmid>10227722</pmid><doi>10.1046/j.1440-1827.1999.00821.x</doi><tpages>8</tpages></addata></record>
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subjects Base Sequence
bile
Bile - chemistry
Bile - cytology
biliary cancer
Biliary Tract - chemistry
Biliary Tract - pathology
Biliary Tract Neoplasms - metabolism
Biliary Tract Neoplasms - pathology
Biopsy
Codon
DNA, Neoplasm - genetics
Genes, p53 - genetics
Genes, ras - genetics
Humans
Immunohistochemistry
K-ras mutation
Mutation
p53 mutation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Tumor Suppressor Protein p53 - analysis
title K-ras codon 12 and p53 mutations in biopsy specimens and bile from biliary tract cancers
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