α-Lipoic acid and ebselen prevent ischemia/reperfusion injury in the rat intestine
Purpose Reactive oxygen species (ROS) and reactive nitrogen species (RNS), generated during tissue reperfusion, are characteristic of ischemia/reperfusion (I/R) injury. We conducted this study to evaluate the protective effect of α-lipoic acid (α-LA) and ebselen against intestinal I/R injury. Method...
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| Veröffentlicht in: | Surgery today (Tokyo, Japan) Japan), 2008-11, Vol.38 (11), p.1029-1035 |
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| container_title | Surgery today (Tokyo, Japan) |
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| creator | Guven, Ahmet Tunc, Turan Topal, Turgut Kul, Mustafa Korkmaz, Ahmet Gundogdu, Gokhan Onguru, Onder Ozturk, Haluk |
| description | Purpose
Reactive oxygen species (ROS) and reactive nitrogen species (RNS), generated during tissue reperfusion, are characteristic of ischemia/reperfusion (I/R) injury. We conducted this study to evaluate the protective effect of α-lipoic acid (α-LA) and ebselen against intestinal I/R injury.
Methods
Forty Sprague-Dawley rats were divided into five groups: a sham-operated group; an I/R group, subjected to intestinal ischemia for 45 min and reperfusion for 3 days; an I/R+α-LA group; an I/R+ebselen group; and an I/R+α-LA+ebselen group. We collected ileal specimens, to measure the tissue levels of malondialdehyde (MDA), protein carbonyl content (PCC), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and to evaluate the histologic changes.
Results
There was a significant decrease in SOD and GPx levels, with an increase in MDA and PCC levels and intestinal mucosal injury in the intestinal I/R group (
P
< 0.05). Superoxide dismutase and GPx levels were significantly higher, MDA and PCC levels were significantly lower, and intestinal injury was significantly less severe in the I/R+α-LA+ebselen group than in the I/R group (
P
< 0.05). Although shortened villi and epithelial lifting were seen in the I/R group, only slight mucosal injury was seen in the treatment groups.
Conclusion
α-Lipoic acid and ebselen played an important role in attenuating I/R injury of the intestine by scavenging ROS and RNS. |
| doi_str_mv | 10.1007/s00595-007-3752-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69724523</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69724523</sourcerecordid><originalsourceid>FETCH-LOGICAL-c395t-3f9a618766543e27220312d5e1c3dea801c1a2453bf846e0474da77a8b2db6d23</originalsourceid><addsrcrecordid>eNp9kE1OwzAQhS0EoqVwADYoK3am_k3iJar4kyqxANaWE0-oq9QJdoLEsbgIZ8JVKrFjNSPN997MPIQuKbmhhBTLSIhUEqcW80IyrI7QnAqeY1ZSfozmRAmKKVN0hs5i3BLCREnIKZrRUslS5myOXn6-8dr1naszUzubGW8zqCK04LM-wCf4IXOx3sDOmWWAHkIzRtf5zPntGL5SyYYNZMEkzA8QB-fhHJ00po1wcagL9HZ_97p6xOvnh6fV7RrXXMkB80aZnJZFnkvBgRWMEU6ZlUBrbsGUhNbUMCF51ZQiByIKYU1RmLJitsot4wt0Pfn2ofsY0269S6dC2xoP3Rh1rookZzyBdALr0MUYoNF9cDsTvjQlep-knpLU-3afpFZJc3UwH6sd2D_FIboEsAmIaeTfIehtNwafHv7H9RctS37x</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69724523</pqid></control><display><type>article</type><title>α-Lipoic acid and ebselen prevent ischemia/reperfusion injury in the rat intestine</title><source>MEDLINE</source><source>SpringerLink_现刊</source><creator>Guven, Ahmet ; Tunc, Turan ; Topal, Turgut ; Kul, Mustafa ; Korkmaz, Ahmet ; Gundogdu, Gokhan ; Onguru, Onder ; Ozturk, Haluk</creator><creatorcontrib>Guven, Ahmet ; Tunc, Turan ; Topal, Turgut ; Kul, Mustafa ; Korkmaz, Ahmet ; Gundogdu, Gokhan ; Onguru, Onder ; Ozturk, Haluk</creatorcontrib><description>Purpose
Reactive oxygen species (ROS) and reactive nitrogen species (RNS), generated during tissue reperfusion, are characteristic of ischemia/reperfusion (I/R) injury. We conducted this study to evaluate the protective effect of α-lipoic acid (α-LA) and ebselen against intestinal I/R injury.
Methods
Forty Sprague-Dawley rats were divided into five groups: a sham-operated group; an I/R group, subjected to intestinal ischemia for 45 min and reperfusion for 3 days; an I/R+α-LA group; an I/R+ebselen group; and an I/R+α-LA+ebselen group. We collected ileal specimens, to measure the tissue levels of malondialdehyde (MDA), protein carbonyl content (PCC), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and to evaluate the histologic changes.
Results
There was a significant decrease in SOD and GPx levels, with an increase in MDA and PCC levels and intestinal mucosal injury in the intestinal I/R group (
P
< 0.05). Superoxide dismutase and GPx levels were significantly higher, MDA and PCC levels were significantly lower, and intestinal injury was significantly less severe in the I/R+α-LA+ebselen group than in the I/R group (
P
< 0.05). Although shortened villi and epithelial lifting were seen in the I/R group, only slight mucosal injury was seen in the treatment groups.
Conclusion
α-Lipoic acid and ebselen played an important role in attenuating I/R injury of the intestine by scavenging ROS and RNS.</description><identifier>ISSN: 0941-1291</identifier><identifier>EISSN: 1436-2813</identifier><identifier>DOI: 10.1007/s00595-007-3752-9</identifier><identifier>PMID: 18958562</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Animals ; Antioxidants - therapeutic use ; Azoles - therapeutic use ; Disease Models, Animal ; Intestinal Diseases - prevention & control ; Intestine, Small ; Male ; Medicine ; Medicine & Public Health ; Organoselenium Compounds - therapeutic use ; Original Article ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - prevention & control ; Surgery ; Surgical Oncology ; Thioctic Acid - therapeutic use</subject><ispartof>Surgery today (Tokyo, Japan), 2008-11, Vol.38 (11), p.1029-1035</ispartof><rights>Springer 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-3f9a618766543e27220312d5e1c3dea801c1a2453bf846e0474da77a8b2db6d23</citedby><cites>FETCH-LOGICAL-c395t-3f9a618766543e27220312d5e1c3dea801c1a2453bf846e0474da77a8b2db6d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00595-007-3752-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00595-007-3752-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18958562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guven, Ahmet</creatorcontrib><creatorcontrib>Tunc, Turan</creatorcontrib><creatorcontrib>Topal, Turgut</creatorcontrib><creatorcontrib>Kul, Mustafa</creatorcontrib><creatorcontrib>Korkmaz, Ahmet</creatorcontrib><creatorcontrib>Gundogdu, Gokhan</creatorcontrib><creatorcontrib>Onguru, Onder</creatorcontrib><creatorcontrib>Ozturk, Haluk</creatorcontrib><title>α-Lipoic acid and ebselen prevent ischemia/reperfusion injury in the rat intestine</title><title>Surgery today (Tokyo, Japan)</title><addtitle>Surg Today</addtitle><addtitle>Surg Today</addtitle><description>Purpose
Reactive oxygen species (ROS) and reactive nitrogen species (RNS), generated during tissue reperfusion, are characteristic of ischemia/reperfusion (I/R) injury. We conducted this study to evaluate the protective effect of α-lipoic acid (α-LA) and ebselen against intestinal I/R injury.
Methods
Forty Sprague-Dawley rats were divided into five groups: a sham-operated group; an I/R group, subjected to intestinal ischemia for 45 min and reperfusion for 3 days; an I/R+α-LA group; an I/R+ebselen group; and an I/R+α-LA+ebselen group. We collected ileal specimens, to measure the tissue levels of malondialdehyde (MDA), protein carbonyl content (PCC), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and to evaluate the histologic changes.
Results
There was a significant decrease in SOD and GPx levels, with an increase in MDA and PCC levels and intestinal mucosal injury in the intestinal I/R group (
P
< 0.05). Superoxide dismutase and GPx levels were significantly higher, MDA and PCC levels were significantly lower, and intestinal injury was significantly less severe in the I/R+α-LA+ebselen group than in the I/R group (
P
< 0.05). Although shortened villi and epithelial lifting were seen in the I/R group, only slight mucosal injury was seen in the treatment groups.
Conclusion
α-Lipoic acid and ebselen played an important role in attenuating I/R injury of the intestine by scavenging ROS and RNS.</description><subject>Animals</subject><subject>Antioxidants - therapeutic use</subject><subject>Azoles - therapeutic use</subject><subject>Disease Models, Animal</subject><subject>Intestinal Diseases - prevention & control</subject><subject>Intestine, Small</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Organoselenium Compounds - therapeutic use</subject><subject>Original Article</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Thioctic Acid - therapeutic use</subject><issn>0941-1291</issn><issn>1436-2813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1OwzAQhS0EoqVwADYoK3am_k3iJar4kyqxANaWE0-oq9QJdoLEsbgIZ8JVKrFjNSPN997MPIQuKbmhhBTLSIhUEqcW80IyrI7QnAqeY1ZSfozmRAmKKVN0hs5i3BLCREnIKZrRUslS5myOXn6-8dr1naszUzubGW8zqCK04LM-wCf4IXOx3sDOmWWAHkIzRtf5zPntGL5SyYYNZMEkzA8QB-fhHJ00po1wcagL9HZ_97p6xOvnh6fV7RrXXMkB80aZnJZFnkvBgRWMEU6ZlUBrbsGUhNbUMCF51ZQiByIKYU1RmLJitsot4wt0Pfn2ofsY0269S6dC2xoP3Rh1rookZzyBdALr0MUYoNF9cDsTvjQlep-knpLU-3afpFZJc3UwH6sd2D_FIboEsAmIaeTfIehtNwafHv7H9RctS37x</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Guven, Ahmet</creator><creator>Tunc, Turan</creator><creator>Topal, Turgut</creator><creator>Kul, Mustafa</creator><creator>Korkmaz, Ahmet</creator><creator>Gundogdu, Gokhan</creator><creator>Onguru, Onder</creator><creator>Ozturk, Haluk</creator><general>Springer Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081101</creationdate><title>α-Lipoic acid and ebselen prevent ischemia/reperfusion injury in the rat intestine</title><author>Guven, Ahmet ; Tunc, Turan ; Topal, Turgut ; Kul, Mustafa ; Korkmaz, Ahmet ; Gundogdu, Gokhan ; Onguru, Onder ; Ozturk, Haluk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-3f9a618766543e27220312d5e1c3dea801c1a2453bf846e0474da77a8b2db6d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antioxidants - therapeutic use</topic><topic>Azoles - therapeutic use</topic><topic>Disease Models, Animal</topic><topic>Intestinal Diseases - prevention & control</topic><topic>Intestine, Small</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Organoselenium Compounds - therapeutic use</topic><topic>Original Article</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Thioctic Acid - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guven, Ahmet</creatorcontrib><creatorcontrib>Tunc, Turan</creatorcontrib><creatorcontrib>Topal, Turgut</creatorcontrib><creatorcontrib>Kul, Mustafa</creatorcontrib><creatorcontrib>Korkmaz, Ahmet</creatorcontrib><creatorcontrib>Gundogdu, Gokhan</creatorcontrib><creatorcontrib>Onguru, Onder</creatorcontrib><creatorcontrib>Ozturk, Haluk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Surgery today (Tokyo, Japan)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guven, Ahmet</au><au>Tunc, Turan</au><au>Topal, Turgut</au><au>Kul, Mustafa</au><au>Korkmaz, Ahmet</au><au>Gundogdu, Gokhan</au><au>Onguru, Onder</au><au>Ozturk, Haluk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>α-Lipoic acid and ebselen prevent ischemia/reperfusion injury in the rat intestine</atitle><jtitle>Surgery today (Tokyo, Japan)</jtitle><stitle>Surg Today</stitle><addtitle>Surg Today</addtitle><date>2008-11-01</date><risdate>2008</risdate><volume>38</volume><issue>11</issue><spage>1029</spage><epage>1035</epage><pages>1029-1035</pages><issn>0941-1291</issn><eissn>1436-2813</eissn><abstract>Purpose
Reactive oxygen species (ROS) and reactive nitrogen species (RNS), generated during tissue reperfusion, are characteristic of ischemia/reperfusion (I/R) injury. We conducted this study to evaluate the protective effect of α-lipoic acid (α-LA) and ebselen against intestinal I/R injury.
Methods
Forty Sprague-Dawley rats were divided into five groups: a sham-operated group; an I/R group, subjected to intestinal ischemia for 45 min and reperfusion for 3 days; an I/R+α-LA group; an I/R+ebselen group; and an I/R+α-LA+ebselen group. We collected ileal specimens, to measure the tissue levels of malondialdehyde (MDA), protein carbonyl content (PCC), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and to evaluate the histologic changes.
Results
There was a significant decrease in SOD and GPx levels, with an increase in MDA and PCC levels and intestinal mucosal injury in the intestinal I/R group (
P
< 0.05). Superoxide dismutase and GPx levels were significantly higher, MDA and PCC levels were significantly lower, and intestinal injury was significantly less severe in the I/R+α-LA+ebselen group than in the I/R group (
P
< 0.05). Although shortened villi and epithelial lifting were seen in the I/R group, only slight mucosal injury was seen in the treatment groups.
Conclusion
α-Lipoic acid and ebselen played an important role in attenuating I/R injury of the intestine by scavenging ROS and RNS.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>18958562</pmid><doi>10.1007/s00595-007-3752-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Surgery today (Tokyo, Japan), 2008-11, Vol.38 (11), p.1029-1035 |
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| source | MEDLINE; SpringerLink_现刊 |
| subjects | Animals Antioxidants - therapeutic use Azoles - therapeutic use Disease Models, Animal Intestinal Diseases - prevention & control Intestine, Small Male Medicine Medicine & Public Health Organoselenium Compounds - therapeutic use Original Article Rats Rats, Sprague-Dawley Reperfusion Injury - prevention & control Surgery Surgical Oncology Thioctic Acid - therapeutic use |
| title | α-Lipoic acid and ebselen prevent ischemia/reperfusion injury in the rat intestine |
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