Comparison of three different methods in monoclonal antibody-based detection of Streptococcus agalactiae protein serotype markers
Surface‐exposed proteins are important serotype markers in Streptococcus agalactiae (group B streptococci; GBS). The proteins include the c proteins cα and cβ, the R4 protein and a protein provisionally called P. For all of these markers, protein‐specific monoclonal antibodies (MAbs) have been gener...
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| Veröffentlicht in: | APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 1999-03, Vol.107 (3), p.263-269 |
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| container_title | APMIS : acta pathologica, microbiologica et immunologica Scandinavica |
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| creator | Moyo, S.R Maeland, J.A Bevanger, L. (Trondheim Univ. (Norway)) |
| description | Surface‐exposed proteins are important serotype markers in Streptococcus agalactiae (group B streptococci; GBS). The proteins include the c proteins cα and cβ, the R4 protein and a protein provisionally called P. For all of these markers, protein‐specific monoclonal antibodies (MAbs) have been generated. We have compared whole‐cell‐based fluorescent antibody testing (FAT), ELISA, and dot blotting for MAb‐based detection of these proteins by testing a panel of 52 GBS isolates of different capsular antigen types. Of a total of 208 observations with each of the tests, positive signalling in the dot assay was observed in 32.2%, with ELISA in 27.8%, and with FAT in 26.4% of the recordings. Discordant results were noted most frequently with the c13 and cα MAbs. In the case of cα the reason for the discordant test results was further examined and it appeared that this could be attributed to low level expression of the cα protein, although structural variations of cα proteins cannot be excluded. Our findings favour dot blotting as the method of choice although we consider all three methods acceptable for serotyping of GBS. |
| doi_str_mv | 10.1111/j.1699-0463.1999.tb01553.x |
| format | Article |
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(Trondheim Univ. (Norway))</creator><creatorcontrib>Moyo, S.R ; Maeland, J.A ; Bevanger, L. (Trondheim Univ. (Norway))</creatorcontrib><description>Surface‐exposed proteins are important serotype markers in Streptococcus agalactiae (group B streptococci; GBS). The proteins include the c proteins cα and cβ, the R4 protein and a protein provisionally called P. For all of these markers, protein‐specific monoclonal antibodies (MAbs) have been generated. We have compared whole‐cell‐based fluorescent antibody testing (FAT), ELISA, and dot blotting for MAb‐based detection of these proteins by testing a panel of 52 GBS isolates of different capsular antigen types. Of a total of 208 observations with each of the tests, positive signalling in the dot assay was observed in 32.2%, with ELISA in 27.8%, and with FAT in 26.4% of the recordings. Discordant results were noted most frequently with the c13 and cα MAbs. In the case of cα the reason for the discordant test results was further examined and it appeared that this could be attributed to low level expression of the cα protein, although structural variations of cα proteins cannot be excluded. Our findings favour dot blotting as the method of choice although we consider all three methods acceptable for serotyping of GBS.</description><identifier>ISSN: 0903-4641</identifier><identifier>EISSN: 1600-0463</identifier><identifier>DOI: 10.1111/j.1699-0463.1999.tb01553.x</identifier><identifier>PMID: 10223298</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Bacterial ; Antibodies, Monoclonal ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; Antigens, Bacterial ; Bacterial Proteins - immunology ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; detection methods ; Enzyme-Linked Immunosorbent Assay - methods ; Evaluation Studies as Topic ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; GENETIC MARKERS ; Humans ; Immunoblotting - methods ; IMMUNOLOGICAL TECHNIQUES ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; Mice ; Microbiology ; MONOCLONAL ANTIBODIES ; protein serotype antigens ; SEROTIPOS ; SEROTYPE ; SEROTYPES ; Serotyping - methods ; STREPTOCOCCUS AGALACTIAE ; Streptococcus agalactiae - classification ; Streptococcus agalactiae - immunology ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS</subject><ispartof>APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 1999-03, Vol.107 (3), p.263-269</ispartof><rights>1999 APMIS</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4593-dc9a02a656298f93c52afc40b27434787ff66fc5157bc25769c271a7265603b83</citedby><cites>FETCH-LOGICAL-c4593-dc9a02a656298f93c52afc40b27434787ff66fc5157bc25769c271a7265603b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1699-0463.1999.tb01553.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1699-0463.1999.tb01553.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1793681$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10223298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moyo, S.R</creatorcontrib><creatorcontrib>Maeland, J.A</creatorcontrib><creatorcontrib>Bevanger, L. (Trondheim Univ. (Norway))</creatorcontrib><title>Comparison of three different methods in monoclonal antibody-based detection of Streptococcus agalactiae protein serotype markers</title><title>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</title><addtitle>APMIS</addtitle><description>Surface‐exposed proteins are important serotype markers in Streptococcus agalactiae (group B streptococci; GBS). The proteins include the c proteins cα and cβ, the R4 protein and a protein provisionally called P. For all of these markers, protein‐specific monoclonal antibodies (MAbs) have been generated. We have compared whole‐cell‐based fluorescent antibody testing (FAT), ELISA, and dot blotting for MAb‐based detection of these proteins by testing a panel of 52 GBS isolates of different capsular antigen types. Of a total of 208 observations with each of the tests, positive signalling in the dot assay was observed in 32.2%, with ELISA in 27.8%, and with FAT in 26.4% of the recordings. Discordant results were noted most frequently with the c13 and cα MAbs. In the case of cα the reason for the discordant test results was further examined and it appeared that this could be attributed to low level expression of the cα protein, although structural variations of cα proteins cannot be excluded. Our findings favour dot blotting as the method of choice although we consider all three methods acceptable for serotyping of GBS.</description><subject>Animals</subject><subject>Antibodies, Bacterial</subject><subject>Antibodies, Monoclonal</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>Antigens, Bacterial</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>detection methods</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Evaluation Studies as Topic</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETIC MARKERS</subject><subject>Humans</subject><subject>Immunoblotting - methods</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>Mice</subject><subject>Microbiology</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>protein serotype antigens</subject><subject>SEROTIPOS</subject><subject>SEROTYPE</subject><subject>SEROTYPES</subject><subject>Serotyping - methods</subject><subject>STREPTOCOCCUS AGALACTIAE</subject><subject>Streptococcus agalactiae - classification</subject><subject>Streptococcus agalactiae - immunology</subject><subject>TECHNIQUE IMMUNOLOGIQUE</subject><subject>TECNICAS INMUNOLOGICAS</subject><issn>0903-4641</issn><issn>1600-0463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkk9v1DAQxSMEokvhI4AshLgl-E9ixxwqVQstiFIqsYij5TjjNksSB9srdo98cxyyAq74YkvzmzdP85xlzwkuSDqvtgXhUua45KwgUsoiNphUFSv297IV4Rj_Lt3PVlhilpe8JCfZoxC2GBNac_EwOyGYUkZlvcp-rt0wad8FNyJnUbzzAKjtrAUPY0QDxDvXBtSNaHCjM70bdY_0GLvGtYe80QFa1EIEE7tF4XP0MEVnnDG7gPSt7nWqaUCTdxGSToD0OEyABu2_gQ-PswdW9wGeHO_T7MvF2836XX716fL9-vwqN2UlWd4aqTHVvOLJt5XMVFRbU-KGipKVohbWcm5NRSrRGFoJLg0VRAuaOjBranaavVx0k5HvOwhRDV0w0Pd6BLcLiktBSUlJAl8voPEuBA9WTb5LZg-KYDUHoLZqDkDNW1ZzAOoYgNqn5mfHKbtmgPaf1mXjCXhxBHQwurdej6YLfzkhGa9nE2cL9qPr4fAfDtT5zUfKWRLIF4EuRNj_EUg7V1wwUamv15fqmm7o5oaV6iLxTxfeaqf0bfoR6s2HWRpjXJeC_QI09byh</recordid><startdate>199903</startdate><enddate>199903</enddate><creator>Moyo, S.R</creator><creator>Maeland, J.A</creator><creator>Bevanger, L. (Trondheim Univ. (Norway))</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199903</creationdate><title>Comparison of three different methods in monoclonal antibody-based detection of Streptococcus agalactiae protein serotype markers</title><author>Moyo, S.R ; Maeland, J.A ; Bevanger, L. (Trondheim Univ. (Norway))</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4593-dc9a02a656298f93c52afc40b27434787ff66fc5157bc25769c271a7265603b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial</topic><topic>Antibodies, Monoclonal</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>Antigens, Bacterial</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>detection methods</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Evaluation Studies as Topic</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETIC MARKERS</topic><topic>Humans</topic><topic>Immunoblotting - methods</topic><topic>IMMUNOLOGICAL TECHNIQUES</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>Mice</topic><topic>Microbiology</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>protein serotype antigens</topic><topic>SEROTIPOS</topic><topic>SEROTYPE</topic><topic>SEROTYPES</topic><topic>Serotyping - methods</topic><topic>STREPTOCOCCUS AGALACTIAE</topic><topic>Streptococcus agalactiae - classification</topic><topic>Streptococcus agalactiae - immunology</topic><topic>TECHNIQUE IMMUNOLOGIQUE</topic><topic>TECNICAS INMUNOLOGICAS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moyo, S.R</creatorcontrib><creatorcontrib>Maeland, J.A</creatorcontrib><creatorcontrib>Bevanger, L. (Trondheim Univ. (Norway))</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moyo, S.R</au><au>Maeland, J.A</au><au>Bevanger, L. (Trondheim Univ. (Norway))</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of three different methods in monoclonal antibody-based detection of Streptococcus agalactiae protein serotype markers</atitle><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle><addtitle>APMIS</addtitle><date>1999-03</date><risdate>1999</risdate><volume>107</volume><issue>3</issue><spage>263</spage><epage>269</epage><pages>263-269</pages><issn>0903-4641</issn><eissn>1600-0463</eissn><abstract>Surface‐exposed proteins are important serotype markers in Streptococcus agalactiae (group B streptococci; GBS). The proteins include the c proteins cα and cβ, the R4 protein and a protein provisionally called P. For all of these markers, protein‐specific monoclonal antibodies (MAbs) have been generated. We have compared whole‐cell‐based fluorescent antibody testing (FAT), ELISA, and dot blotting for MAb‐based detection of these proteins by testing a panel of 52 GBS isolates of different capsular antigen types. Of a total of 208 observations with each of the tests, positive signalling in the dot assay was observed in 32.2%, with ELISA in 27.8%, and with FAT in 26.4% of the recordings. Discordant results were noted most frequently with the c13 and cα MAbs. In the case of cα the reason for the discordant test results was further examined and it appeared that this could be attributed to low level expression of the cα protein, although structural variations of cα proteins cannot be excluded. Our findings favour dot blotting as the method of choice although we consider all three methods acceptable for serotyping of GBS.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10223298</pmid><doi>10.1111/j.1699-0463.1999.tb01553.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0903-4641 |
| ispartof | APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 1999-03, Vol.107 (3), p.263-269 |
| issn | 0903-4641 1600-0463 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies, Bacterial Antibodies, Monoclonal ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES Antigens, Bacterial Bacterial Proteins - immunology Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences detection methods Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology GENETIC MARKERS Humans Immunoblotting - methods IMMUNOLOGICAL TECHNIQUES MARCADORES GENETICOS MARQUEUR GENETIQUE Mice Microbiology MONOCLONAL ANTIBODIES protein serotype antigens SEROTIPOS SEROTYPE SEROTYPES Serotyping - methods STREPTOCOCCUS AGALACTIAE Streptococcus agalactiae - classification Streptococcus agalactiae - immunology TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS |
| title | Comparison of three different methods in monoclonal antibody-based detection of Streptococcus agalactiae protein serotype markers |
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