Hydroquinone as a Buffer Additive for Suppression of Bubbles Formed by Electrochemical Oxidation of the CE Buffer at the Outlet Electrode in Capillary Electrophoresis/Electrospray Ionization-Mass Spectrometry
Hydroquinone was found to suppress bubble formation at the outlet electrode of a sheathless capillary electrophoresis/electrospray ionization-mass spectrometer by replacing the oxidation of water (2H2O(l) ↔ O2(g) + 4H+ + 4e) with that of more easily oxidized hydroquinone (hydroquinone ↔ p-benzoquino...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1999-04, Vol.71 (8), p.1658-1661 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Moini, Mehdi Cao, Ping Bard, Allen J |
| description | Hydroquinone was found to suppress bubble formation at the outlet electrode of a sheathless capillary electrophoresis/electrospray ionization-mass spectrometer by replacing the oxidation of water (2H2O(l) ↔ O2(g) + 4H+ + 4e) with that of more easily oxidized hydroquinone (hydroquinone ↔ p-benzoquinone + 2H+ + 2e). Formation of p-benzoquinone replaces the formation of oxygen gas, effectively suppressing gas bubble formation. Several electrode materials, including platinum, gold-coated stainless steel, and stainless steel wires, were tested. However, hydroquinone suppressed bubbles only at the platinum electrode. Combination of the in-capillary electrode sheathless interface using platinum wire, hydroquinone as a buffer additive, and pressure programming at the inlet of the capillary electrophoresis provided a rugged high efficiency interface for analysis of protein digests using CE/ESI-MS. |
| doi_str_mv | 10.1021/ac9811266 |
| format | Article |
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| ispartof | Analytical chemistry (Washington), 1999-04, Vol.71 (8), p.1658-1661 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Analytical chemistry Buffers Chemicals Chemistry Electrochemical methods Electrochemistry Electrodes Electrophoresis, Capillary - instrumentation Exact sciences and technology Hydroquinones - chemistry Mass Spectrometry - instrumentation Oxidation Oxidation-Reduction |
| title | Hydroquinone as a Buffer Additive for Suppression of Bubbles Formed by Electrochemical Oxidation of the CE Buffer at the Outlet Electrode in Capillary Electrophoresis/Electrospray Ionization-Mass Spectrometry |
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