Aberrant Metabolic Sialylation of Recombinant Proteins Expressed in Chinese Hamster Ovary Cells in High Productivity Cultures

The incorporation of sialic acid into therapeutic recombinant glycoprotein expressed in Chinese hamster ovary (CHO) cells during growth in large bioreactors (10 l) has been monitored under high productivity conditions induced by the presence of sodium butyrate. Samples of the bioreactor culture (∼4...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemical and biophysical research communications 1999-04, Vol.258 (1), p.132-137
Hauptverfasser:	Santell, Lydia, Ryll, Thomas, Etcheverry, Tina, Santoris, Michael, Dutina, George, Wang, Angie, Gunson, Jane, Warner, Thomas G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques CHO Cells Chromatography, Affinity Chromatography, High Pressure Liquid Cricetinae Hexosamines - metabolism N-Acetylneuraminic Acid - metabolism Recombinant Proteins - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!