Novel BNIP1 variants and their interaction with BCL2 family members
By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1-b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown...
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| Veröffentlicht in: | FEBS letters 1999-04, Vol.448 (1), p.23-27 |
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| creator | Zhang, Hong Heim, Jutta Meyhack, Bernd |
| description | By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1-b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl-xL). These interactions are BH3-independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3-only members. |
| doi_str_mv | 10.1016/S0014-5793(99)00335-X |
| format | Article |
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The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl-xL). These interactions are BH3-independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3-only members.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)00335-X</identifier><identifier>PMID: 10217402</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Alternative Splicing ; Amino Acid Sequence ; Base Sequence ; BAX ; bcl-2-Associated X Protein ; bcl-X Protein ; BCL2 ; BCL2A1, BCL2-related protein A1 (previously Bfl-1) ; BCL2L1 ; BCL2L1, BCL2-like 1 (previously Bcl-xL) ; BH3, BCL2 homology region 3 ; BNIP1 ; BNIP1, BCL2/adenovirus E1B 19 kDa interacting protein 1 (previously Nip1) ; BNIP1vs, BNIP1 splice variants, including BNIP1-a, BNIP1-b and BNIP1-c (previously: Nip1s1, Nip1s2 and Nip1s3) ; CAT, chloramphenicol acetyltransferase ; DNA, Complementary ; EST, expressed sequence tag ; GST, glutathione S-transferase ; Humans ; Interaction ; Molecular Sequence Data ; PAGE, polyacrylamide gel electrophoresis ; PCR, polymerase chain reaction ; Proteins - genetics ; Proteins - metabolism ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; STS, sequence tagged site ; Variant</subject><ispartof>FEBS letters, 1999-04, Vol.448 (1), p.23-27</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 448 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473X-381ce4249c10c3cf9533e815edb5dcd69f8ac643f6ce63997fdd2bd6f593c75d3</citedby><cites>FETCH-LOGICAL-c473X-381ce4249c10c3cf9533e815edb5dcd69f8ac643f6ce63997fdd2bd6f593c75d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2899%2900335-X$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S001457939900335X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3536,27903,27904,45553,45554,46387,46811,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10217402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hong</creatorcontrib><creatorcontrib>Heim, Jutta</creatorcontrib><creatorcontrib>Meyhack, Bernd</creatorcontrib><title>Novel BNIP1 variants and their interaction with BCL2 family members</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1-b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl-xL). These interactions are BH3-independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3-only members.</description><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>BAX</subject><subject>bcl-2-Associated X Protein</subject><subject>bcl-X Protein</subject><subject>BCL2</subject><subject>BCL2A1, BCL2-related protein A1 (previously Bfl-1)</subject><subject>BCL2L1</subject><subject>BCL2L1, BCL2-like 1 (previously Bcl-xL)</subject><subject>BH3, BCL2 homology region 3</subject><subject>BNIP1</subject><subject>BNIP1, BCL2/adenovirus E1B 19 kDa interacting protein 1 (previously Nip1)</subject><subject>BNIP1vs, BNIP1 splice variants, including BNIP1-a, BNIP1-b and BNIP1-c (previously: Nip1s1, Nip1s2 and Nip1s3)</subject><subject>CAT, chloramphenicol acetyltransferase</subject><subject>DNA, Complementary</subject><subject>EST, expressed sequence tag</subject><subject>GST, glutathione S-transferase</subject><subject>Humans</subject><subject>Interaction</subject><subject>Molecular Sequence Data</subject><subject>PAGE, polyacrylamide gel electrophoresis</subject><subject>PCR, polymerase chain reaction</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>STS, sequence tagged site</subject><subject>Variant</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1LAzEQhoMotlZ_gpKT6GE12exmNyexpbVCqYIKvYU0maWR_ajJtqX_3m23iDc9DZl55p3wIHRJyR0llN-_EUKjIE4EuxHilhDG4mB2hLo0TVjAIp4eo-4P0kFn3n-S5p1ScYo6lIQ0iUjYRYNptYYc96fPrxSvlbOqrD1WpcH1AqzDtqzBKV3bqsQbWy9wfzAJcaYKm29xAcUcnD9HJ5nKPVwcag99jIbvg3EweXl6HjxOAh0lbBawlGqIwkhoSjTTmYgZg5TGYOax0YaLLFWaRyzjGjgTIsmMCeeGZ7FgOokN66HrNnfpqq8V-FoW1mvIc1VCtfKSi4RymvIGjFtQu8p7B5lcOlsot5WUyJ09ubcnd2qkEHJvT86avavDgdW8APNrq9XVAOMW2Ngctv9LlaNhP9xPdgMh9u3drYc2ChpjawtOem2h1GCsA11LU9k_fvsNaY6Siw</recordid><startdate>19990402</startdate><enddate>19990402</enddate><creator>Zhang, Hong</creator><creator>Heim, Jutta</creator><creator>Meyhack, Bernd</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990402</creationdate><title>Novel BNIP1 variants and their interaction with BCL2 family members</title><author>Zhang, Hong ; Heim, Jutta ; Meyhack, Bernd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473X-381ce4249c10c3cf9533e815edb5dcd69f8ac643f6ce63997fdd2bd6f593c75d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>BAX</topic><topic>bcl-2-Associated X Protein</topic><topic>bcl-X Protein</topic><topic>BCL2</topic><topic>BCL2A1, BCL2-related protein A1 (previously Bfl-1)</topic><topic>BCL2L1</topic><topic>BCL2L1, BCL2-like 1 (previously Bcl-xL)</topic><topic>BH3, BCL2 homology region 3</topic><topic>BNIP1</topic><topic>BNIP1, BCL2/adenovirus E1B 19 kDa interacting protein 1 (previously Nip1)</topic><topic>BNIP1vs, BNIP1 splice variants, including BNIP1-a, BNIP1-b and BNIP1-c (previously: Nip1s1, Nip1s2 and Nip1s3)</topic><topic>CAT, chloramphenicol acetyltransferase</topic><topic>DNA, Complementary</topic><topic>EST, expressed sequence tag</topic><topic>GST, glutathione S-transferase</topic><topic>Humans</topic><topic>Interaction</topic><topic>Molecular Sequence Data</topic><topic>PAGE, polyacrylamide gel electrophoresis</topic><topic>PCR, polymerase chain reaction</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>STS, sequence tagged site</topic><topic>Variant</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hong</creatorcontrib><creatorcontrib>Heim, Jutta</creatorcontrib><creatorcontrib>Meyhack, Bernd</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hong</au><au>Heim, Jutta</au><au>Meyhack, Bernd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel BNIP1 variants and their interaction with BCL2 family members</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-04-02</date><risdate>1999</risdate><volume>448</volume><issue>1</issue><spage>23</spage><epage>27</epage><pages>23-27</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1-b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl-xL). These interactions are BH3-independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3-only members.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10217402</pmid><doi>10.1016/S0014-5793(99)00335-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1999-04, Vol.448 (1), p.23-27 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Alternative Splicing Amino Acid Sequence Base Sequence BAX bcl-2-Associated X Protein bcl-X Protein BCL2 BCL2A1, BCL2-related protein A1 (previously Bfl-1) BCL2L1 BCL2L1, BCL2-like 1 (previously Bcl-xL) BH3, BCL2 homology region 3 BNIP1 BNIP1, BCL2/adenovirus E1B 19 kDa interacting protein 1 (previously Nip1) BNIP1vs, BNIP1 splice variants, including BNIP1-a, BNIP1-b and BNIP1-c (previously: Nip1s1, Nip1s2 and Nip1s3) CAT, chloramphenicol acetyltransferase DNA, Complementary EST, expressed sequence tag GST, glutathione S-transferase Humans Interaction Molecular Sequence Data PAGE, polyacrylamide gel electrophoresis PCR, polymerase chain reaction Proteins - genetics Proteins - metabolism Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism STS, sequence tagged site Variant |
| title | Novel BNIP1 variants and their interaction with BCL2 family members |
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