Characterization of the DNA Binding Properties of the bHLH Domain of Deadpan to Single and Tandem Sites

The basic helix−loop−helix domain of the Drosophila transcription factor Deadpan (Dpn) was prepared by total chemical protein synthesis in order to characterize its DNA binding properties. Circular dichroism spectroscopy was used to correlate structural changes in Dpn with physiologically relevant m...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemistry (Easton) 1999-04, Vol.38 (16), p.5138-5146
Hauptverfasser:	Winston, Rachel L, Millar, David P, Gottesfeld, Joel M, Kent, Stephen B. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Basic Helix-Loop-Helix Transcription Factors Binding Sites Circular Dichroism DNA - metabolism DNA-Binding Proteins - chemical synthesis DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Drosophila Drosophila Proteins Fluorescence Polarization Helix-Loop-Helix Motifs Kinetics Magnesium Chloride - chemistry Molecular Sequence Data Nuclear Proteins - chemical synthesis Nuclear Proteins - chemistry Nuclear Proteins - metabolism Peptide Fragments - chemical synthesis Peptide Fragments - chemistry Peptide Fragments - metabolism Potassium Chloride - chemistry Protein Binding Repetitive Sequences, Amino Acid Spectrometry, Fluorescence Ultracentrifugation
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!