Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan
Previously, we reported that an isolate of novel type of Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an e...
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| Veröffentlicht in: | Veterinary parasitology 2008-11, Vol.158 (1), p.44-50 |
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| creator | Matsubayashi, Makoto Nagano, Saki Kita, Toshimasa Narushima, Tsuguto Kimata, Isao Iseki, Motohiro Hajiri, Tomoya Tani, Hiroyuki Sasai, Kazumi Baba, Eiichiroh |
| description | Previously, we reported that an isolate of novel type of
Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to
C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an extensive investigation of
Cryptosporidium infections in adult cattle in Japan from 2004 to 2007. Consequently,
Cryptosporidium sp. were detected in 12 of the 205 cattle examined (5.9%), and partial sequences of the
Cryptosporidium oocyst wall protein (COWP) gene in all isolates were identical to those of the previously reported data for
C. andersoni whereas two signals were observed in the sequence of the partial 18S rRNA gene in all the isolates. In transmission studies using five of the isolates, they all infected SCID mice. Modified multiplex PCR using DNA of a single oocyst isolated from the infected SCID mice revealed that the partial sequences in the 18S rRNA gene of 40–80% of 10 isolates were identical to the Type A genotype of
C. andersoni and those of other samples were identical to the Type B genotype. These results suggested that the
C. andersoni novel type is widespread in cattle throughout Japan, and have multiple copies (Types A and B) in the 18S rRNA gene. |
| doi_str_mv | 10.1016/j.vetpar.2008.08.021 |
| format | Article |
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Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to
C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an extensive investigation of
Cryptosporidium infections in adult cattle in Japan from 2004 to 2007. Consequently,
Cryptosporidium sp. were detected in 12 of the 205 cattle examined (5.9%), and partial sequences of the
Cryptosporidium oocyst wall protein (COWP) gene in all isolates were identical to those of the previously reported data for
C. andersoni whereas two signals were observed in the sequence of the partial 18S rRNA gene in all the isolates. In transmission studies using five of the isolates, they all infected SCID mice. Modified multiplex PCR using DNA of a single oocyst isolated from the infected SCID mice revealed that the partial sequences in the 18S rRNA gene of 40–80% of 10 isolates were identical to the Type A genotype of
C. andersoni and those of other samples were identical to the Type B genotype. These results suggested that the
C. andersoni novel type is widespread in cattle throughout Japan, and have multiple copies (Types A and B) in the 18S rRNA gene.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2008.08.021</identifier><identifier>PMID: 18922640</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Biological Assay - veterinary ; Cattle ; Cattle Diseases - parasitology ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium andersoni ; Cryptosporidium andersoni novel type ; disease surveillance ; feces ; Feces - parasitology ; Female ; Genotype ; Japan ; Male ; Mice ; Mice, SCID ; microbial detection ; microbial genetics ; molecular sequence data ; nucleotide sequences ; oocysts ; Oocysts - classification ; Polymerase Chain Reaction - veterinary ; RNA, Ribosomal, 18S - genetics</subject><ispartof>Veterinary parasitology, 2008-11, Vol.158 (1), p.44-50</ispartof><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-66abb85de7949833656fb33928bf0e9e2eb777705746509de8555930f0156dab3</citedby><cites>FETCH-LOGICAL-c481t-66abb85de7949833656fb33928bf0e9e2eb777705746509de8555930f0156dab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2008.08.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18922640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsubayashi, Makoto</creatorcontrib><creatorcontrib>Nagano, Saki</creatorcontrib><creatorcontrib>Kita, Toshimasa</creatorcontrib><creatorcontrib>Narushima, Tsuguto</creatorcontrib><creatorcontrib>Kimata, Isao</creatorcontrib><creatorcontrib>Iseki, Motohiro</creatorcontrib><creatorcontrib>Hajiri, Tomoya</creatorcontrib><creatorcontrib>Tani, Hiroyuki</creatorcontrib><creatorcontrib>Sasai, Kazumi</creatorcontrib><creatorcontrib>Baba, Eiichiroh</creatorcontrib><title>Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Previously, we reported that an isolate of novel type of
Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to
C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an extensive investigation of
Cryptosporidium infections in adult cattle in Japan from 2004 to 2007. Consequently,
Cryptosporidium sp. were detected in 12 of the 205 cattle examined (5.9%), and partial sequences of the
Cryptosporidium oocyst wall protein (COWP) gene in all isolates were identical to those of the previously reported data for
C. andersoni whereas two signals were observed in the sequence of the partial 18S rRNA gene in all the isolates. In transmission studies using five of the isolates, they all infected SCID mice. Modified multiplex PCR using DNA of a single oocyst isolated from the infected SCID mice revealed that the partial sequences in the 18S rRNA gene of 40–80% of 10 isolates were identical to the Type A genotype of
C. andersoni and those of other samples were identical to the Type B genotype. These results suggested that the
C. andersoni novel type is widespread in cattle throughout Japan, and have multiple copies (Types A and B) in the 18S rRNA gene.</description><subject>Animals</subject><subject>Biological Assay - veterinary</subject><subject>Cattle</subject><subject>Cattle Diseases - parasitology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium andersoni</subject><subject>Cryptosporidium andersoni novel type</subject><subject>disease surveillance</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Genotype</subject><subject>Japan</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>microbial detection</subject><subject>microbial genetics</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>oocysts</subject><subject>Oocysts - classification</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>RNA, Ribosomal, 18S - genetics</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1q3DAURkVJaKZJ3qC0XnXnydWv5U0gDGnaMCSLdNZCtq-LBo_lSvLAvH1tPNBdKi5IgvN9XA4hnymsKVB1t18fMQ02rBmAXs_D6AeyorrgOZMSLsgKOIhcAC2uyKcY9wAgQBUfyRXVJWNKwIq8PGGPydW2y-IYjnjKfJv1_ohdlk4Dzr9NOA3Jx8EH17jxkNm-wRB97zLXZ7VNqcP59WwH29-Qy9Z2EW_P9zXZfX_8tfmRb1-ffm4etnktNE25UraqtGywKEWpOVdStRXnJdNVC1giw6qYDshCKAllg1pKWXJogUrV2Ipfk29L7xD8nxFjMgcXa-w626Mfo1FlAZRq9V9wkqeoFHQCxQLWwccYsDVDcAcbToaCmYWbvVmEzxlt5mFz7Mu5f6wO2PwLnQ1PwNcFaK039ndw0ezeGFA-VRZMSDYR9wuBk7Cjw2Bi7bCvsXEB62Qa797f4S_2z5vd</recordid><startdate>20081125</startdate><enddate>20081125</enddate><creator>Matsubayashi, Makoto</creator><creator>Nagano, Saki</creator><creator>Kita, Toshimasa</creator><creator>Narushima, Tsuguto</creator><creator>Kimata, Isao</creator><creator>Iseki, Motohiro</creator><creator>Hajiri, Tomoya</creator><creator>Tani, Hiroyuki</creator><creator>Sasai, Kazumi</creator><creator>Baba, Eiichiroh</creator><general>Elsevier B.V</general><general>Amsterdam; 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Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to
C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an extensive investigation of
Cryptosporidium infections in adult cattle in Japan from 2004 to 2007. Consequently,
Cryptosporidium sp. were detected in 12 of the 205 cattle examined (5.9%), and partial sequences of the
Cryptosporidium oocyst wall protein (COWP) gene in all isolates were identical to those of the previously reported data for
C. andersoni whereas two signals were observed in the sequence of the partial 18S rRNA gene in all the isolates. In transmission studies using five of the isolates, they all infected SCID mice. Modified multiplex PCR using DNA of a single oocyst isolated from the infected SCID mice revealed that the partial sequences in the 18S rRNA gene of 40–80% of 10 isolates were identical to the Type A genotype of
C. andersoni and those of other samples were identical to the Type B genotype. These results suggested that the
C. andersoni novel type is widespread in cattle throughout Japan, and have multiple copies (Types A and B) in the 18S rRNA gene.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18922640</pmid><doi>10.1016/j.vetpar.2008.08.021</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2008-11, Vol.158 (1), p.44-50 |
| issn | 0304-4017 1873-2550 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological Assay - veterinary Cattle Cattle Diseases - parasitology Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium andersoni Cryptosporidium andersoni novel type disease surveillance feces Feces - parasitology Female Genotype Japan Male Mice Mice, SCID microbial detection microbial genetics molecular sequence data nucleotide sequences oocysts Oocysts - classification Polymerase Chain Reaction - veterinary RNA, Ribosomal, 18S - genetics |
| title | Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan |
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