Potential Threat of a New Pathotype of Papaya leaf distortion mosaic virus Infecting Transgenic Papaya Resistant to Papaya ringspot virus
A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mo...
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| Veröffentlicht in: | Phytopathology 2008-07, Vol.98 (7), p.848-856 |
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| creator | Bau, H.J Kung, Y.J Raja, J.A.J Chan, S.J Chen, K.C Chen, Y.K Wu, H.W Yeh, S.D |
| description | A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other 18 plant species tested. Virions studied under electron microscope exhibited morphology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' noncoding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere. |
| doi_str_mv | 10.1094/PHYTO-98-7-0848 |
| format | Article |
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The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other 18 plant species tested. Virions studied under electron microscope exhibited morphology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' noncoding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere.</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/PHYTO-98-7-0848</identifier><identifier>PMID: 18943262</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>Biological and medical sciences ; Capsid Proteins - genetics ; Carica - genetics ; Carica - virology ; Carica papaya ; coat proteins ; disease resistance ; disease severity ; Fundamental and applied biological sciences. Psychology ; genetic resistance ; host plants ; host range ; Immunity, Innate ; Japan ; microbial genetics ; Microscopy, Electron, Transmission ; molecular sequence data ; nucleotide sequences ; Papaya leaf distortion mosaic virus ; Papaya ringspot virus ; papayas ; pathogen identification ; pathotypes ; Phylogeny ; Phytopathology. Animal pests. Plant and forest protection ; Plant Diseases - virology ; plant diseases and disorders ; Plant viruses and viroids ; Plants, Genetically Modified - virology ; Potyvirus ; Potyvirus - classification ; Potyvirus - genetics ; Potyvirus - isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction ; signs and symptoms (plants) ; Taiwan ; transgenic plants ; Virion - isolation & purification ; Virion - ultrastructure</subject><ispartof>Phytopathology, 2008-07, Vol.98 (7), p.848-856</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-379a09d1bf53ac805f29612d8d5399d16cd0357a2850cd8cc091ead2c3b39e663</citedby><cites>FETCH-LOGICAL-c380t-379a09d1bf53ac805f29612d8d5399d16cd0357a2850cd8cc091ead2c3b39e663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3711,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20449174$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18943262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bau, H.J</creatorcontrib><creatorcontrib>Kung, Y.J</creatorcontrib><creatorcontrib>Raja, J.A.J</creatorcontrib><creatorcontrib>Chan, S.J</creatorcontrib><creatorcontrib>Chen, K.C</creatorcontrib><creatorcontrib>Chen, Y.K</creatorcontrib><creatorcontrib>Wu, H.W</creatorcontrib><creatorcontrib>Yeh, S.D</creatorcontrib><title>Potential Threat of a New Pathotype of Papaya leaf distortion mosaic virus Infecting Transgenic Papaya Resistant to Papaya ringspot virus</title><title>Phytopathology</title><addtitle>Phytopathology</addtitle><description>A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other 18 plant species tested. Virions studied under electron microscope exhibited morphology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' noncoding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere.</description><subject>Biological and medical sciences</subject><subject>Capsid Proteins - genetics</subject><subject>Carica - genetics</subject><subject>Carica - virology</subject><subject>Carica papaya</subject><subject>coat proteins</subject><subject>disease resistance</subject><subject>disease severity</subject><subject>Fundamental and applied biological sciences. 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Plant and forest protection</subject><subject>Plant Diseases - virology</subject><subject>plant diseases and disorders</subject><subject>Plant viruses and viroids</subject><subject>Plants, Genetically Modified - virology</subject><subject>Potyvirus</subject><subject>Potyvirus - classification</subject><subject>Potyvirus - genetics</subject><subject>Potyvirus - isolation & purification</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>signs and symptoms (plants)</subject><subject>Taiwan</subject><subject>transgenic plants</subject><subject>Virion - isolation & purification</subject><subject>Virion - ultrastructure</subject><issn>0031-949X</issn><issn>1943-7684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFu1DAQBmALgehSOHMDX-AWOraT2D6iCtpKFV3BVoKTNevY26BsnNpeqn0E3hovG-DIydL4--cwPyEvGbxjoOuz5eW31U2lVSUrULV6RBZM16KSraofkwWAYJWu9dcT8iyl7wAgVdM-JSdMFcVbviA_lyG7Mfc40NVddJhp8BTpJ_dAl5jvQt5P7jBa4oR7pINDT7s-5RBzH0a6DQl7S3_0cZfo1eidzf24oauIY9q4sXzNwc8ulRSOmebwZxYLTVPIx_hz8sTjkNyL-T0ltx8_rM4vq-ubi6vz99eVFQpyJaRG0B1b-0agVdB4rlvGO9U1Qpd5azsQjUSuGrCdshY0c9hxK9ZCu7YVp-Ttce8Uw_3OpWy2fbJuGHB0YZdMqyWAkuy_kOlGCGBNgWdHaGNIKTpvpthvMe4NA3OoyfyuyWhlpDnUVBKv5tW79dZ1__zcSwFvZoDJ4uDLPW2f_joOda2ZrIt7fXQeg8FNLOb2CwcmADTXgkvxC0-apjg</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Bau, H.J</creator><creator>Kung, Y.J</creator><creator>Raja, J.A.J</creator><creator>Chan, S.J</creator><creator>Chen, K.C</creator><creator>Chen, Y.K</creator><creator>Wu, H.W</creator><creator>Yeh, S.D</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080701</creationdate><title>Potential Threat of a New Pathotype of Papaya leaf distortion mosaic virus Infecting Transgenic Papaya Resistant to Papaya ringspot virus</title><author>Bau, H.J ; Kung, Y.J ; Raja, J.A.J ; Chan, S.J ; Chen, K.C ; Chen, Y.K ; Wu, H.W ; Yeh, S.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-379a09d1bf53ac805f29612d8d5399d16cd0357a2850cd8cc091ead2c3b39e663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Capsid Proteins - genetics</topic><topic>Carica - genetics</topic><topic>Carica - virology</topic><topic>Carica papaya</topic><topic>coat proteins</topic><topic>disease resistance</topic><topic>disease severity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic resistance</topic><topic>host plants</topic><topic>host range</topic><topic>Immunity, Innate</topic><topic>Japan</topic><topic>microbial genetics</topic><topic>Microscopy, Electron, Transmission</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>Papaya leaf distortion mosaic virus</topic><topic>Papaya ringspot virus</topic><topic>papayas</topic><topic>pathogen identification</topic><topic>pathotypes</topic><topic>Phylogeny</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Diseases - virology</topic><topic>plant diseases and disorders</topic><topic>Plant viruses and viroids</topic><topic>Plants, Genetically Modified - virology</topic><topic>Potyvirus</topic><topic>Potyvirus - classification</topic><topic>Potyvirus - genetics</topic><topic>Potyvirus - isolation & purification</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>signs and symptoms (plants)</topic><topic>Taiwan</topic><topic>transgenic plants</topic><topic>Virion - isolation & purification</topic><topic>Virion - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bau, H.J</creatorcontrib><creatorcontrib>Kung, Y.J</creatorcontrib><creatorcontrib>Raja, J.A.J</creatorcontrib><creatorcontrib>Chan, S.J</creatorcontrib><creatorcontrib>Chen, K.C</creatorcontrib><creatorcontrib>Chen, Y.K</creatorcontrib><creatorcontrib>Wu, H.W</creatorcontrib><creatorcontrib>Yeh, S.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bau, H.J</au><au>Kung, Y.J</au><au>Raja, J.A.J</au><au>Chan, S.J</au><au>Chen, K.C</au><au>Chen, Y.K</au><au>Wu, H.W</au><au>Yeh, S.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential Threat of a New Pathotype of Papaya leaf distortion mosaic virus Infecting Transgenic Papaya Resistant to Papaya ringspot virus</atitle><jtitle>Phytopathology</jtitle><addtitle>Phytopathology</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>98</volume><issue>7</issue><spage>848</spage><epage>856</epage><pages>848-856</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><coden>PHYTAJ</coden><abstract>A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other 18 plant species tested. Virions studied under electron microscope exhibited morphology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' noncoding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere.</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><pmid>18943262</pmid><doi>10.1094/PHYTO-98-7-0848</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | Biological and medical sciences Capsid Proteins - genetics Carica - genetics Carica - virology Carica papaya coat proteins disease resistance disease severity Fundamental and applied biological sciences. Psychology genetic resistance host plants host range Immunity, Innate Japan microbial genetics Microscopy, Electron, Transmission molecular sequence data nucleotide sequences Papaya leaf distortion mosaic virus Papaya ringspot virus papayas pathogen identification pathotypes Phylogeny Phytopathology. Animal pests. Plant and forest protection Plant Diseases - virology plant diseases and disorders Plant viruses and viroids Plants, Genetically Modified - virology Potyvirus Potyvirus - classification Potyvirus - genetics Potyvirus - isolation & purification Reverse Transcriptase Polymerase Chain Reaction signs and symptoms (plants) Taiwan transgenic plants Virion - isolation & purification Virion - ultrastructure |
| title | Potential Threat of a New Pathotype of Papaya leaf distortion mosaic virus Infecting Transgenic Papaya Resistant to Papaya ringspot virus |
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