Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations
A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E. multilocularis coproantigens and somatic worm antigens...
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| Veröffentlicht in: | The Journal of parasitology 1999-02, Vol.85 (1), p.115-121 |
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| creator | Deplazes, P Alther, P Tanner, I Thompson, R.C.A Eckert, J |
| description | A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E. multilocularis coproantigens and somatic worm antigens, respectively. In experimentally infected dogs and cats, coproantigens were first detectable 6-17 days postinfection (PI) in samples of 8 dogs (worm burdens at necropsy: 6,330-43,200) and from 11 days PI onward in samples of 5 cats infected with 20-6,833 worms. After anthelmintic treatment of 4 dogs and 5 cats at day 20 PI, coproantigen excretion disappeared within 3-5 days. The sensitivity of the ELISA was 83.6% in 55 foxes infected with 4-60,000 E. multilocularis, but reached 93.3% in the 45 foxes harboring more than 20 worms. The EM-ELISA was used in surveys of "normal" dog and cat populations in Switzerland. Among 660 dogs and 263 cats, 5 dogs and 2 cats exhibited a positive reaction. In 2 of these dogs (0.30%) and 1 cat (0.38%), intestinal E. multilocularis infections were confirmed by necropsy, polymerase chain reaction PCR, or both. The specificities of the ELISA in these groups were found to be 99.5% and 99.6%, respectively, if positive ELISA results that could not be confirmed by other methods were classified as "false positive" reactions. |
| doi_str_mv | 10.2307/3285713 |
| format | Article |
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In experimentally infected dogs and cats, coproantigens were first detectable 6-17 days postinfection (PI) in samples of 8 dogs (worm burdens at necropsy: 6,330-43,200) and from 11 days PI onward in samples of 5 cats infected with 20-6,833 worms. After anthelmintic treatment of 4 dogs and 5 cats at day 20 PI, coproantigen excretion disappeared within 3-5 days. The sensitivity of the ELISA was 83.6% in 55 foxes infected with 4-60,000 E. multilocularis, but reached 93.3% in the 45 foxes harboring more than 20 worms. The EM-ELISA was used in surveys of "normal" dog and cat populations in Switzerland. Among 660 dogs and 263 cats, 5 dogs and 2 cats exhibited a positive reaction. In 2 of these dogs (0.30%) and 1 cat (0.38%), intestinal E. multilocularis infections were confirmed by necropsy, polymerase chain reaction PCR, or both. The specificities of the ELISA in these groups were found to be 99.5% and 99.6%, respectively, if positive ELISA results that could not be confirmed by other methods were classified as "false positive" reactions.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3285713</identifier><identifier>PMID: 10207375</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Animals, Domestic ; Animals, Wild ; antibodies ; antigen detection ; Antigens ; Antigens, Helminth - analysis ; Biological and medical sciences ; Cat Diseases - diagnosis ; Cat Diseases - parasitology ; Cats ; Coproantigens ; Cross Reactions ; digesta ; Dog Diseases - diagnosis ; Dog Diseases - parasitology ; Dogs ; echinococcosis ; Echinococcosis - diagnosis ; Echinococcosis - parasitology ; Echinococcosis - veterinary ; Echinococcus - immunology ; Echinococcus - isolation & purification ; Echinococcus multilocularis ; Eggs ; Enzyme linked immunosorbent assay ; Experimental helminthic diseases. Models ; experimental infections ; feces ; Feces - parasitology ; Female ; Foxes ; Foxes - parasitology ; Helminthic diseases ; Infections ; Infectious diseases ; Intestinal Diseases, Parasitic - diagnosis ; Intestinal Diseases, Parasitic - parasitology ; Intestinal Diseases, Parasitic - veterinary ; Male ; Medical sciences ; Memory interference ; Parasitic diseases ; Parasitology ; polyclonal antibodies ; sandwich elisa ; Sensitivity and Specificity ; serodiagnosis ; Therapeutics-Diagnostics</subject><ispartof>The Journal of parasitology, 1999-02, Vol.85 (1), p.115-121</ispartof><rights>Copyright 1999 American Society of Parasitologists</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-72d40e344b49597a4bac8467a33800cb33c5b524453ac48949239b95a700a99d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3285713$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3285713$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1762756$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10207375$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deplazes, P</creatorcontrib><creatorcontrib>Alther, P</creatorcontrib><creatorcontrib>Tanner, I</creatorcontrib><creatorcontrib>Thompson, R.C.A</creatorcontrib><creatorcontrib>Eckert, J</creatorcontrib><title>Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E. multilocularis coproantigens and somatic worm antigens, respectively. In experimentally infected dogs and cats, coproantigens were first detectable 6-17 days postinfection (PI) in samples of 8 dogs (worm burdens at necropsy: 6,330-43,200) and from 11 days PI onward in samples of 5 cats infected with 20-6,833 worms. After anthelmintic treatment of 4 dogs and 5 cats at day 20 PI, coproantigen excretion disappeared within 3-5 days. The sensitivity of the ELISA was 83.6% in 55 foxes infected with 4-60,000 E. multilocularis, but reached 93.3% in the 45 foxes harboring more than 20 worms. The EM-ELISA was used in surveys of "normal" dog and cat populations in Switzerland. Among 660 dogs and 263 cats, 5 dogs and 2 cats exhibited a positive reaction. In 2 of these dogs (0.30%) and 1 cat (0.38%), intestinal E. multilocularis infections were confirmed by necropsy, polymerase chain reaction PCR, or both. The specificities of the ELISA in these groups were found to be 99.5% and 99.6%, respectively, if positive ELISA results that could not be confirmed by other methods were classified as "false positive" reactions.</description><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Animals, Wild</subject><subject>antibodies</subject><subject>antigen detection</subject><subject>Antigens</subject><subject>Antigens, Helminth - analysis</subject><subject>Biological and medical sciences</subject><subject>Cat Diseases - diagnosis</subject><subject>Cat Diseases - parasitology</subject><subject>Cats</subject><subject>Coproantigens</subject><subject>Cross Reactions</subject><subject>digesta</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>echinococcosis</subject><subject>Echinococcosis - diagnosis</subject><subject>Echinococcosis - parasitology</subject><subject>Echinococcosis - veterinary</subject><subject>Echinococcus - immunology</subject><subject>Echinococcus - isolation & purification</subject><subject>Echinococcus multilocularis</subject><subject>Eggs</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Experimental helminthic diseases. Models</subject><subject>experimental infections</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Foxes</subject><subject>Foxes - parasitology</subject><subject>Helminthic diseases</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Intestinal Diseases, Parasitic - diagnosis</subject><subject>Intestinal Diseases, Parasitic - parasitology</subject><subject>Intestinal Diseases, Parasitic - veterinary</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Memory interference</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>polyclonal antibodies</subject><subject>sandwich elisa</subject><subject>Sensitivity and Specificity</subject><subject>serodiagnosis</subject><subject>Therapeutics-Diagnostics</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBmALgehSEP8AfEBwaWDiseP4WFXlQ6rEAXqOJo6zuCT2EjtSl1-PV7tSOcHB8uWZ1_I7jL2s4b1A0B9QtErX-IhtaoO6EijVY7YBEKJCNOqMPUvpDgBUOU_ZWQ0CNGq1YffX9ocP0UZr18Tndcp-inadaPGJ27hbIoXsty7wwWVns4-B93vuwu_97KrJh59u4H6e1xBTXHoXMqeUaM994GO8v-BD3F5wCgO3lPku7kr0ISQ9Z09GmpJ7cbrP2e3H6-9Xn6ubr5--XF3eVFa2JldaDBIcStlLo4wm2ZNtZaMJsQWwPaJVvRJSKqTDhDQCTW8UaQAyZsBz9vaYW77ya3Upd7NP1k0TBRfX1DWmMW2D-F9Y61J0a6DAd0dol5jS4sZut_iZln1XQ3fYRnfaRpGvTpFrP7vhL3esv4A3J0DJ0jQuFKxPD043Qqvmgd2lHJd_PPf6yEaKHW3LBrvbbwJqBGFAqlLRH5MopeA</recordid><startdate>19990201</startdate><enddate>19990201</enddate><creator>Deplazes, P</creator><creator>Alther, P</creator><creator>Tanner, I</creator><creator>Thompson, R.C.A</creator><creator>Eckert, J</creator><general>American Society of Parasitologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19990201</creationdate><title>Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations</title><author>Deplazes, P ; Alther, P ; Tanner, I ; Thompson, R.C.A ; Eckert, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-72d40e344b49597a4bac8467a33800cb33c5b524453ac48949239b95a700a99d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Animals, Wild</topic><topic>antibodies</topic><topic>antigen detection</topic><topic>Antigens</topic><topic>Antigens, Helminth - analysis</topic><topic>Biological and medical sciences</topic><topic>Cat Diseases - diagnosis</topic><topic>Cat Diseases - parasitology</topic><topic>Cats</topic><topic>Coproantigens</topic><topic>Cross Reactions</topic><topic>digesta</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>echinococcosis</topic><topic>Echinococcosis - diagnosis</topic><topic>Echinococcosis - parasitology</topic><topic>Echinococcosis - veterinary</topic><topic>Echinococcus - immunology</topic><topic>Echinococcus - isolation & purification</topic><topic>Echinococcus multilocularis</topic><topic>Eggs</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Experimental helminthic diseases. Models</topic><topic>experimental infections</topic><topic>feces</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Foxes</topic><topic>Foxes - parasitology</topic><topic>Helminthic diseases</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Intestinal Diseases, Parasitic - diagnosis</topic><topic>Intestinal Diseases, Parasitic - parasitology</topic><topic>Intestinal Diseases, Parasitic - veterinary</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Memory interference</topic><topic>Parasitic diseases</topic><topic>Parasitology</topic><topic>polyclonal antibodies</topic><topic>sandwich elisa</topic><topic>Sensitivity and Specificity</topic><topic>serodiagnosis</topic><topic>Therapeutics-Diagnostics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deplazes, P</creatorcontrib><creatorcontrib>Alther, P</creatorcontrib><creatorcontrib>Tanner, I</creatorcontrib><creatorcontrib>Thompson, R.C.A</creatorcontrib><creatorcontrib>Eckert, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deplazes, P</au><au>Alther, P</au><au>Tanner, I</au><au>Thompson, R.C.A</au><au>Eckert, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>1999-02-01</date><risdate>1999</risdate><volume>85</volume><issue>1</issue><spage>115</spage><epage>121</epage><pages>115-121</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E. multilocularis coproantigens and somatic worm antigens, respectively. In experimentally infected dogs and cats, coproantigens were first detectable 6-17 days postinfection (PI) in samples of 8 dogs (worm burdens at necropsy: 6,330-43,200) and from 11 days PI onward in samples of 5 cats infected with 20-6,833 worms. After anthelmintic treatment of 4 dogs and 5 cats at day 20 PI, coproantigen excretion disappeared within 3-5 days. The sensitivity of the ELISA was 83.6% in 55 foxes infected with 4-60,000 E. multilocularis, but reached 93.3% in the 45 foxes harboring more than 20 worms. The EM-ELISA was used in surveys of "normal" dog and cat populations in Switzerland. Among 660 dogs and 263 cats, 5 dogs and 2 cats exhibited a positive reaction. In 2 of these dogs (0.30%) and 1 cat (0.38%), intestinal E. multilocularis infections were confirmed by necropsy, polymerase chain reaction PCR, or both. The specificities of the ELISA in these groups were found to be 99.5% and 99.6%, respectively, if positive ELISA results that could not be confirmed by other methods were classified as "false positive" reactions.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>10207375</pmid><doi>10.2307/3285713</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of parasitology, 1999-02, Vol.85 (1), p.115-121 |
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| subjects | Animals Animals, Domestic Animals, Wild antibodies antigen detection Antigens Antigens, Helminth - analysis Biological and medical sciences Cat Diseases - diagnosis Cat Diseases - parasitology Cats Coproantigens Cross Reactions digesta Dog Diseases - diagnosis Dog Diseases - parasitology Dogs echinococcosis Echinococcosis - diagnosis Echinococcosis - parasitology Echinococcosis - veterinary Echinococcus - immunology Echinococcus - isolation & purification Echinococcus multilocularis Eggs Enzyme linked immunosorbent assay Experimental helminthic diseases. Models experimental infections feces Feces - parasitology Female Foxes Foxes - parasitology Helminthic diseases Infections Infectious diseases Intestinal Diseases, Parasitic - diagnosis Intestinal Diseases, Parasitic - parasitology Intestinal Diseases, Parasitic - veterinary Male Medical sciences Memory interference Parasitic diseases Parasitology polyclonal antibodies sandwich elisa Sensitivity and Specificity serodiagnosis Therapeutics-Diagnostics |
| title | Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations |
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