High-resolution electron cryomicroscopy of macromolecular assemblies
Electron cryomicroscopy is a high-resolution imaging technique that is particularly appropriate for the structural determination of large macromolecular assemblies, which are difficult to study by X-ray crystallography or NMR spectroscopy. For some biological molecules that form two-dimensional crys...
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| Veröffentlicht in: | Trends in cell biology 1999-04, Vol.9 (4), p.154-159 |
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| container_title | Trends in cell biology |
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| creator | Chiu, Wah McGough, Amy Sherman, Michael B. Schmid, Michael F. |
| description | Electron cryomicroscopy is a high-resolution imaging technique that is particularly appropriate for the structural determination of large macromolecular assemblies, which are difficult to study by X-ray crystallography or NMR spectroscopy. For some biological molecules that form two-dimensional crystals, the application of electron cryomicroscopy and image reconstruction can help elucidate structures at atomic resolution. In instances where crystals cannot be formed, atomic-resolution information can be obtained by combining high-resolution structures of individual components determined by X-ray crystallography or NMR with image-derived reconstructions at moderate resolution. This can provide unique and crucial information on the mechanisms of these complexes. Finally, image reconstructions can be used to augment X-ray studies by providing initial models that facilitate phasing of crystals of large macromolecular machines such as ribosomes and viruses. |
| doi_str_mv | 10.1016/S0962-8924(99)01511-1 |
| format | Article |
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| ispartof | Trends in cell biology, 1999-04, Vol.9 (4), p.154-159 |
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| subjects | Actin Cytoskeleton - ultrastructure Animals Cryoelectron Microscopy - methods Crystallization Crystallography, X-Ray electron microscopy electron tomography Image Processing, Computer-Assisted Macromolecular Substances Magnetic Resonance Spectroscopy Male microscopy Models, Molecular Organelles - ultrastructure Ribosomes - ultrastructure Spermatozoa - ultrastructure |
| title | High-resolution electron cryomicroscopy of macromolecular assemblies |
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