Endostatin Induces Endothelial Cell Apoptosis
Endostatin, a carboxyl-terminal fragment of collagen XVIII, has been shown to regress tumors in mice. In this study, we have analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary artery endothelial cells caused apoptosis, as...
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| Veröffentlicht in: | The Journal of biological chemistry 1999-04, Vol.274 (17), p.11721-11726 |
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| container_end_page | 11726 |
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| container_issue | 17 |
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| container_title | The Journal of biological chemistry |
| container_volume | 274 |
| creator | Dhanabal, M Ramchandran, R Waterman, M J Lu, H Knebelmann, B Segal, M Sukhatme, V P |
| description | Endostatin, a carboxyl-terminal fragment of collagen XVIII, has been shown to regress tumors in mice. In this study, we have
analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary
artery endothelial cells caused apoptosis, as demonstrated by three methods, annexin V-fluorescein isothiocyanate staining,
caspase 3, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay. Moreover, addition of endostatin
led to a marked reduction of the Bcl-2 and Bcl-X L anti-apoptotic protein, whereas Bax protein levels were unaffected. These effects were not seen in several nonendothelial
cells. Collectively, these findings provide important mechanistic insight into endostatin action. |
| doi_str_mv | 10.1074/jbc.274.17.11721 |
| format | Article |
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analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary
artery endothelial cells caused apoptosis, as demonstrated by three methods, annexin V-fluorescein isothiocyanate staining,
caspase 3, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay. Moreover, addition of endostatin
led to a marked reduction of the Bcl-2 and Bcl-X L anti-apoptotic protein, whereas Bax protein levels were unaffected. These effects were not seen in several nonendothelial
cells. Collectively, these findings provide important mechanistic insight into endostatin action.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.274.17.11721</identifier><identifier>PMID: 10206987</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Apoptosis - drug effects ; Biological Transport ; Cattle ; Cell Membrane - metabolism ; Cell Nucleus - drug effects ; Cell Nucleus - metabolism ; Collagen - pharmacology ; Collagen Type XVIII ; Endostatins ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; In Situ Nick-End Labeling ; Mice ; Peptide Fragments - pharmacology ; Phosphatidylserines - metabolism</subject><ispartof>The Journal of biological chemistry, 1999-04, Vol.274 (17), p.11721-11726</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-db27988dbd40b721eb4135a66ae416e2a1565d8d9a743156953f0e197e64ac803</citedby><cites>FETCH-LOGICAL-c366t-db27988dbd40b721eb4135a66ae416e2a1565d8d9a743156953f0e197e64ac803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10206987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dhanabal, M</creatorcontrib><creatorcontrib>Ramchandran, R</creatorcontrib><creatorcontrib>Waterman, M J</creatorcontrib><creatorcontrib>Lu, H</creatorcontrib><creatorcontrib>Knebelmann, B</creatorcontrib><creatorcontrib>Segal, M</creatorcontrib><creatorcontrib>Sukhatme, V P</creatorcontrib><title>Endostatin Induces Endothelial Cell Apoptosis</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Endostatin, a carboxyl-terminal fragment of collagen XVIII, has been shown to regress tumors in mice. In this study, we have
analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary
artery endothelial cells caused apoptosis, as demonstrated by three methods, annexin V-fluorescein isothiocyanate staining,
caspase 3, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay. Moreover, addition of endostatin
led to a marked reduction of the Bcl-2 and Bcl-X L anti-apoptotic protein, whereas Bax protein levels were unaffected. These effects were not seen in several nonendothelial
cells. Collectively, these findings provide important mechanistic insight into endostatin action.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Biological Transport</subject><subject>Cattle</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Collagen - pharmacology</subject><subject>Collagen Type XVIII</subject><subject>Endostatins</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>In Situ Nick-End Labeling</subject><subject>Mice</subject><subject>Peptide Fragments - pharmacology</subject><subject>Phosphatidylserines - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkL1PwzAQxS0EoqWwM6EMiC3Blzh2PFZVgUqVWEBis5z4SlzlizgR4r-vSzrALXc6vff09CPkFmgEVLDHfV5EsWARiAhAxHBG5kCzJExS-Dgnc0pjCGWcZjNy5dye-mESLskMaEy5zMSchOvGtG7Qg22CTWPGAl1wfA0lVlZXwQqrKlh2bTe0zrprcrHTlcOb016Q96f12-ol3L4-b1bLbVgknA-hyWMhs8zkhtHc18KcQZJqzjUy4BhrSHlqMiO1YIm_ZZrsKIIUyJkuMposyMOU2_Xt14huULV1ha-iG2xHp7jkUjDOvJBOwqJvnetxp7re1rr_UUDVEZHyiJRHpECoX0TecnfKHvMazR_DxMQL7idBaT_Lb9ujym1blFj_zzkA6ndsQw</recordid><startdate>19990423</startdate><enddate>19990423</enddate><creator>Dhanabal, M</creator><creator>Ramchandran, R</creator><creator>Waterman, M J</creator><creator>Lu, H</creator><creator>Knebelmann, B</creator><creator>Segal, M</creator><creator>Sukhatme, V P</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990423</creationdate><title>Endostatin Induces Endothelial Cell Apoptosis</title><author>Dhanabal, M ; Ramchandran, R ; Waterman, M J ; Lu, H ; Knebelmann, B ; Segal, M ; Sukhatme, V P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-db27988dbd40b721eb4135a66ae416e2a1565d8d9a743156953f0e197e64ac803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Biological Transport</topic><topic>Cattle</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Collagen - pharmacology</topic><topic>Collagen Type XVIII</topic><topic>Endostatins</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>In Situ Nick-End Labeling</topic><topic>Mice</topic><topic>Peptide Fragments - pharmacology</topic><topic>Phosphatidylserines - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dhanabal, M</creatorcontrib><creatorcontrib>Ramchandran, R</creatorcontrib><creatorcontrib>Waterman, M J</creatorcontrib><creatorcontrib>Lu, H</creatorcontrib><creatorcontrib>Knebelmann, B</creatorcontrib><creatorcontrib>Segal, M</creatorcontrib><creatorcontrib>Sukhatme, V P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dhanabal, M</au><au>Ramchandran, R</au><au>Waterman, M J</au><au>Lu, H</au><au>Knebelmann, B</au><au>Segal, M</au><au>Sukhatme, V P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endostatin Induces Endothelial Cell Apoptosis</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1999-04-23</date><risdate>1999</risdate><volume>274</volume><issue>17</issue><spage>11721</spage><epage>11726</epage><pages>11721-11726</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Endostatin, a carboxyl-terminal fragment of collagen XVIII, has been shown to regress tumors in mice. In this study, we have
analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary
artery endothelial cells caused apoptosis, as demonstrated by three methods, annexin V-fluorescein isothiocyanate staining,
caspase 3, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay. Moreover, addition of endostatin
led to a marked reduction of the Bcl-2 and Bcl-X L anti-apoptotic protein, whereas Bax protein levels were unaffected. These effects were not seen in several nonendothelial
cells. Collectively, these findings provide important mechanistic insight into endostatin action.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>10206987</pmid><doi>10.1074/jbc.274.17.11721</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Apoptosis - drug effects Biological Transport Cattle Cell Membrane - metabolism Cell Nucleus - drug effects Cell Nucleus - metabolism Collagen - pharmacology Collagen Type XVIII Endostatins Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism In Situ Nick-End Labeling Mice Peptide Fragments - pharmacology Phosphatidylserines - metabolism |
| title | Endostatin Induces Endothelial Cell Apoptosis |
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