PspA can form large scaffolds in Escherichia coli
The phage shock protein A (PspA) of Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30–40 nm diameter with a scaffold-like arrangement of protein subunits at the surface. Th...
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| Veröffentlicht in: | FEBS letters 2008-10, Vol.582 (25), p.3585-3589 |
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| container_title | FEBS letters |
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| creator | Standar, Kerstin Mehner, Denise Osadnik, Hendrik Berthelmann, Felix Hause, Gerd Lünsdorf, Heinrich Brüser, Thomas |
| description | The phage shock protein A (PspA) of
Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30–40
nm diameter with a scaffold-like arrangement of protein subunits at the surface. The ‘PspA-scaffold’ is the basic structure that is common to all particles. The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area. |
| doi_str_mv | 10.1016/j.febslet.2008.09.002 |
| format | Article |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - ultrastructure Cell Membrane - chemistry Cell Membrane - metabolism EFTEM energy-filtered transmission electron microscopy Escherichia coli Escherichia coli - metabolism Escherichia coli - ultrastructure Escherichia coli Proteins - chemistry Escherichia coli Proteins - isolation & purification Escherichia coli Proteins - ultrastructure Heat-Shock Proteins - chemistry Heat-Shock Proteins - isolation & purification Heat-Shock Proteins - ultrastructure Membrane stabilization Membrane stress Phage shock protein |
| title | PspA can form large scaffolds in Escherichia coli |
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