Direct and nondestructive verification of PNA immobilization using click chemistry

The fluorogenic 1,3-Huisgen dipolar cycloaddition reaction was used as part of a novel immobilization strategy of PNA capture probes on a microarray. By using this click chemistry, azidocoumarin-anchored PNA probes were immobilized on phenyl acetylene-modified glass slides with the simultaneous gene...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 2008-11, Vol.376 (4), p.633-636
Hauptverfasser:	Lim, Seong Yoon, Chung, Won-young, Lee, Hyeon Kyu, Park, Myeong Soon, Park, Hyun Gyu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylene - analogs & derivatives Acetylene - chemistry Azides - chemistry Breast Neoplasms - diagnosis Breast Neoplasms - genetics Click chemistry Coumarins - chemistry DNA chip DNA Mutational Analysis - methods Fluorescent Dyes - chemistry Genes, BRCA1 Glass - chemistry Humans Immobilization Oligonucleotide Array Sequence Analysis - methods Oligonucleotide Probes - chemistry Peptide nucleic acid Peptide Nucleic Acids - chemistry Spectrometry, Fluorescence - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!