Atopy patch test reactions are associated with T lymphocyte-mediated allergen-specific immune responses in atopic dermatitis
Background Positive test reactions to epicutaneous application of aeroallergens served as a model of ‘early’ eczema in atopic dermatitis (AD) in a number of dermato‐immunological studies. However, no quantitative evaluation has been performed so far comparing specific T‐cell activation parameters in...
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| Veröffentlicht in: | Clinical and experimental allergy 1999-04, Vol.29 (4), p.513-521 |
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| container_title | Clinical and experimental allergy |
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| creator | WISTOKAT-WÜLFING, A SCHMIDT, P DARSOW, U RING, J KAPP, A WERFEL, T |
| description | Background
Positive test reactions to epicutaneous application of aeroallergens served as a model of ‘early’ eczema in atopic dermatitis (AD) in a number of dermato‐immunological studies. However, no quantitative evaluation has been performed so far comparing specific T‐cell activation parameters in the peripheral blood of AD patients with positive or negative atopy patch tests (APT).
Objective
The purpose of this study was to investigate specific immunological parameters in patients with atopic dermatitis showing positive or negative atopy patch tests reactions.
Methods
APT results (n = 96) were compared with allergen‐specific IgE, specific lymphocyte proliferation, and the expression of ‘activation’ markers on peripheral blood T‐cells upon in vitro stimulation with house dust mite, cat or grass pollen allergens.
Results
Only a subpopulation (48%) of patients sensitized to aeroallergens (i.e. specific IgE > 0.7 kU/L) developed APT‐reactions to the corresponding allergen. APT reactions were, however, significantly associated with allergen specific lymphocyte proliferation (p |
| doi_str_mv | 10.1046/j.1365-2222.1999.00510.x |
| format | Article |
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Positive test reactions to epicutaneous application of aeroallergens served as a model of ‘early’ eczema in atopic dermatitis (AD) in a number of dermato‐immunological studies. However, no quantitative evaluation has been performed so far comparing specific T‐cell activation parameters in the peripheral blood of AD patients with positive or negative atopy patch tests (APT).
Objective
The purpose of this study was to investigate specific immunological parameters in patients with atopic dermatitis showing positive or negative atopy patch tests reactions.
Methods
APT results (n = 96) were compared with allergen‐specific IgE, specific lymphocyte proliferation, and the expression of ‘activation’ markers on peripheral blood T‐cells upon in vitro stimulation with house dust mite, cat or grass pollen allergens.
Results
Only a subpopulation (48%) of patients sensitized to aeroallergens (i.e. specific IgE > 0.7 kU/L) developed APT‐reactions to the corresponding allergen. APT reactions were, however, significantly associated with allergen specific lymphocyte proliferation (p < 0.0001), and a higher number of CD54+ or CD30+ T‐cells (p < 0.05) upon in vitro stimulation.
Conclusion
The association of delayed skin reactions with allergen specific T‐cell parameters in the blood points to an immunologically mediated mechanism leading to positive reactions in the APT.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1046/j.1365-2222.1999.00510.x</identifier><identifier>PMID: 10202366</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Allergens - immunology ; Allergic diseases ; Animals ; atopic dermatitis ; atopy patch test ; Biological and medical sciences ; cat epithelia ; Dermatitis, Atopic - immunology ; Flow Cytometry ; grass pollen ; house dust mite ; Humans ; Immunopathology ; Lymphocyte Activation ; Medical sciences ; Mites - immunology ; Patch Tests ; Skin allergic diseases. Stinging insect allergies ; T-Lymphocytes - immunology</subject><ispartof>Clinical and experimental allergy, 1999-04, Vol.29 (4), p.513-521</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Apr 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4580-53402084d4653c82739ee38b81a904ecad0cc56d3c5b92122817cc2396460daa3</citedby><cites>FETCH-LOGICAL-c4580-53402084d4653c82739ee38b81a904ecad0cc56d3c5b92122817cc2396460daa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2222.1999.00510.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2222.1999.00510.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1753003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10202366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WISTOKAT-WÜLFING, A</creatorcontrib><creatorcontrib>SCHMIDT, P</creatorcontrib><creatorcontrib>DARSOW, U</creatorcontrib><creatorcontrib>RING, J</creatorcontrib><creatorcontrib>KAPP, A</creatorcontrib><creatorcontrib>WERFEL, T</creatorcontrib><title>Atopy patch test reactions are associated with T lymphocyte-mediated allergen-specific immune responses in atopic dermatitis</title><title>Clinical and experimental allergy</title><addtitle>Clinical & Experimental Allergy</addtitle><description>Background
Positive test reactions to epicutaneous application of aeroallergens served as a model of ‘early’ eczema in atopic dermatitis (AD) in a number of dermato‐immunological studies. However, no quantitative evaluation has been performed so far comparing specific T‐cell activation parameters in the peripheral blood of AD patients with positive or negative atopy patch tests (APT).
Objective
The purpose of this study was to investigate specific immunological parameters in patients with atopic dermatitis showing positive or negative atopy patch tests reactions.
Methods
APT results (n = 96) were compared with allergen‐specific IgE, specific lymphocyte proliferation, and the expression of ‘activation’ markers on peripheral blood T‐cells upon in vitro stimulation with house dust mite, cat or grass pollen allergens.
Results
Only a subpopulation (48%) of patients sensitized to aeroallergens (i.e. specific IgE > 0.7 kU/L) developed APT‐reactions to the corresponding allergen. APT reactions were, however, significantly associated with allergen specific lymphocyte proliferation (p < 0.0001), and a higher number of CD54+ or CD30+ T‐cells (p < 0.05) upon in vitro stimulation.
Conclusion
The association of delayed skin reactions with allergen specific T‐cell parameters in the blood points to an immunologically mediated mechanism leading to positive reactions in the APT.</description><subject>Allergens - immunology</subject><subject>Allergic diseases</subject><subject>Animals</subject><subject>atopic dermatitis</subject><subject>atopy patch test</subject><subject>Biological and medical sciences</subject><subject>cat epithelia</subject><subject>Dermatitis, Atopic - immunology</subject><subject>Flow Cytometry</subject><subject>grass pollen</subject><subject>house dust mite</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>Lymphocyte Activation</subject><subject>Medical sciences</subject><subject>Mites - immunology</subject><subject>Patch Tests</subject><subject>Skin allergic diseases. Stinging insect allergies</subject><subject>T-Lymphocytes - immunology</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV2LEzEUhoMobl39CxJEvJuaTD4mAW9qWbtCUdAVwZuQZk5t6nxtkrId8MebOmUVr8xNAu_znnNyXoQwJXNKuHy9n1MmRVHmM6da6zkhImvHB2h2LzxEM6IFLyql-QV6EuOeEMKEVo_RBSUlKZmUM_RzkfphxINNbocTxIQDWJd830VsA2AbY--8TVDjO592-AY3YzvsejcmKFqoJ8k2DYTv0BVxAOe33mHftocOcrE45FIQse-wza2yVENobfLJx6fo0dY2EZ6d70v05d3VzfK6WH9cvV8u1oXjQpFCMJ7nVbzmUjCnyoppAKY2ilpNODhbE-eErJkTG13SslS0cq5kWnJJamvZJXo11R1Cf3vInzStjw6axnbQH6KRWmrKeJXBF_-A-_4QujybyWtWSsmqzJCaIBf6GANszRB8a8NoKDGneMzenFIwpxROPm1-x2OO2fr8XP-wycv7yzjlkYGXZ8BGZ5ttsJ3z8Q9XCZZDzNibCbvzDYz_3d8srxb5ke3FZPcxwfHebsMPIytWCfP1w8pcs0_i7Wr9zXxmvwDwWbrG</recordid><startdate>199904</startdate><enddate>199904</enddate><creator>WISTOKAT-WÜLFING, A</creator><creator>SCHMIDT, P</creator><creator>DARSOW, U</creator><creator>RING, J</creator><creator>KAPP, A</creator><creator>WERFEL, T</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>199904</creationdate><title>Atopy patch test reactions are associated with T lymphocyte-mediated allergen-specific immune responses in atopic dermatitis</title><author>WISTOKAT-WÜLFING, A ; SCHMIDT, P ; DARSOW, U ; RING, J ; KAPP, A ; WERFEL, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4580-53402084d4653c82739ee38b81a904ecad0cc56d3c5b92122817cc2396460daa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Allergens - immunology</topic><topic>Allergic diseases</topic><topic>Animals</topic><topic>atopic dermatitis</topic><topic>atopy patch test</topic><topic>Biological and medical sciences</topic><topic>cat epithelia</topic><topic>Dermatitis, Atopic - immunology</topic><topic>Flow Cytometry</topic><topic>grass pollen</topic><topic>house dust mite</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>Lymphocyte Activation</topic><topic>Medical sciences</topic><topic>Mites - immunology</topic><topic>Patch Tests</topic><topic>Skin allergic diseases. Stinging insect allergies</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WISTOKAT-WÜLFING, A</creatorcontrib><creatorcontrib>SCHMIDT, P</creatorcontrib><creatorcontrib>DARSOW, U</creatorcontrib><creatorcontrib>RING, J</creatorcontrib><creatorcontrib>KAPP, A</creatorcontrib><creatorcontrib>WERFEL, T</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WISTOKAT-WÜLFING, A</au><au>SCHMIDT, P</au><au>DARSOW, U</au><au>RING, J</au><au>KAPP, A</au><au>WERFEL, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atopy patch test reactions are associated with T lymphocyte-mediated allergen-specific immune responses in atopic dermatitis</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clinical & Experimental Allergy</addtitle><date>1999-04</date><risdate>1999</risdate><volume>29</volume><issue>4</issue><spage>513</spage><epage>521</epage><pages>513-521</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Background
Positive test reactions to epicutaneous application of aeroallergens served as a model of ‘early’ eczema in atopic dermatitis (AD) in a number of dermato‐immunological studies. However, no quantitative evaluation has been performed so far comparing specific T‐cell activation parameters in the peripheral blood of AD patients with positive or negative atopy patch tests (APT).
Objective
The purpose of this study was to investigate specific immunological parameters in patients with atopic dermatitis showing positive or negative atopy patch tests reactions.
Methods
APT results (n = 96) were compared with allergen‐specific IgE, specific lymphocyte proliferation, and the expression of ‘activation’ markers on peripheral blood T‐cells upon in vitro stimulation with house dust mite, cat or grass pollen allergens.
Results
Only a subpopulation (48%) of patients sensitized to aeroallergens (i.e. specific IgE > 0.7 kU/L) developed APT‐reactions to the corresponding allergen. APT reactions were, however, significantly associated with allergen specific lymphocyte proliferation (p < 0.0001), and a higher number of CD54+ or CD30+ T‐cells (p < 0.05) upon in vitro stimulation.
Conclusion
The association of delayed skin reactions with allergen specific T‐cell parameters in the blood points to an immunologically mediated mechanism leading to positive reactions in the APT.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>10202366</pmid><doi>10.1046/j.1365-2222.1999.00510.x</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0954-7894 |
| ispartof | Clinical and experimental allergy, 1999-04, Vol.29 (4), p.513-521 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Allergens - immunology Allergic diseases Animals atopic dermatitis atopy patch test Biological and medical sciences cat epithelia Dermatitis, Atopic - immunology Flow Cytometry grass pollen house dust mite Humans Immunopathology Lymphocyte Activation Medical sciences Mites - immunology Patch Tests Skin allergic diseases. Stinging insect allergies T-Lymphocytes - immunology |
| title | Atopy patch test reactions are associated with T lymphocyte-mediated allergen-specific immune responses in atopic dermatitis |
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