Pharmacokinetics of gastrodin and its metabolite p-hydroxybenzyl alcohol in rat blood, brain and bile by microdialysis coupled to LC–MS/MS
Gastrodin is a pharmacologically active substance isolated from Gastrodia elata Blume with sedation, anti-convulsion and anti-epilepsy activities. A rapid and sensitive liquid chromatography technique coupled to tandem mass spectrometry (LC–MS/MS) system was developed to determine gastrodin and its...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2008-11, Vol.48 (3), p.909-917 |
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| creator | Lin, Lei-Chwen Chen, Yen-Fei Lee, Wen-Chuan Wu, Yu-Tse Tsai, Tung-Hu |
| description | Gastrodin is a pharmacologically active substance isolated from
Gastrodia elata Blume with sedation, anti-convulsion and anti-epilepsy activities. A rapid and sensitive liquid chromatography technique coupled to tandem mass spectrometry (LC–MS/MS) system was developed to determine gastrodin and its metabolite
p-hydroxybenzyl alcohol (HBA) in rat blood, brain and bile collected using microdialysis technique. The analytes were separated using a reversed phase column (4.6
mm
×
150
mm, 5
μm). The mobile phase for column separation was 30% methanol with a flow rate of 0.6
mL/min. As a post-column addition, 1% ammonium hydroxide solution (in methanol) was additionally pumped via a T-connection using a chromatographic pump (BAS PM-80, USA) at a flow rate of 0.2
mL/min after the column separation. A LC–MS/MS system equipped with a negative electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor
m/
z 285.0
→
122.9 and
m/
z 123.0
→
105.0 transitions for gastrodin and HBA, respectively. The lower limit of quantification (LLoQ) for gastrodin and HBA were 0.5 and 2
ng/mL, respectively. The calibration curves were linear over the range of 0.5–5000
ng/mL and 2–1000
ng/mL for gastrodin and HBA with a coefficient of determination >0.995, respectively. This selective and sensitive method is useful for the determination of gastrodin and HBA and in the pharmacokinetic studies of these compounds. |
| doi_str_mv | 10.1016/j.jpba.2008.07.013 |
| format | Article |
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Gastrodia elata Blume with sedation, anti-convulsion and anti-epilepsy activities. A rapid and sensitive liquid chromatography technique coupled to tandem mass spectrometry (LC–MS/MS) system was developed to determine gastrodin and its metabolite
p-hydroxybenzyl alcohol (HBA) in rat blood, brain and bile collected using microdialysis technique. The analytes were separated using a reversed phase column (4.6
mm
×
150
mm, 5
μm). The mobile phase for column separation was 30% methanol with a flow rate of 0.6
mL/min. As a post-column addition, 1% ammonium hydroxide solution (in methanol) was additionally pumped via a T-connection using a chromatographic pump (BAS PM-80, USA) at a flow rate of 0.2
mL/min after the column separation. A LC–MS/MS system equipped with a negative electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor
m/
z 285.0
→
122.9 and
m/
z 123.0
→
105.0 transitions for gastrodin and HBA, respectively. The lower limit of quantification (LLoQ) for gastrodin and HBA were 0.5 and 2
ng/mL, respectively. The calibration curves were linear over the range of 0.5–5000
ng/mL and 2–1000
ng/mL for gastrodin and HBA with a coefficient of determination >0.995, respectively. This selective and sensitive method is useful for the determination of gastrodin and HBA and in the pharmacokinetic studies of these compounds.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2008.07.013</identifier><identifier>PMID: 18757149</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Benzyl Alcohols - blood ; Benzyl Alcohols - chemistry ; Benzyl Alcohols - isolation & purification ; Benzyl Alcohols - metabolism ; Benzyl Alcohols - pharmacokinetics ; Bile - metabolism ; Biological and medical sciences ; Brain - metabolism ; Calibration ; Chromatography, Liquid - methods ; Fundamental and applied biological sciences. Psychology ; Gastrodia elata ; Gastrodia elata Blume ; Gastrodin ; General pharmacology ; Glucosides - chemistry ; Glucosides - isolation & purification ; Glucosides - metabolism ; Glucosides - pharmacokinetics ; Herbal medicine ; Male ; Medical sciences ; Microdialysis ; Molecular Structure ; Pharmacokinetics ; Pharmacology. Drug treatments ; Plant Roots - chemistry ; Rats ; Rats, Sprague-Dawley ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem Mass Spectrometry - methods ; Time Factors</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2008-11, Vol.48 (3), p.909-917</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-bc79e179c8588d99a5165b54a04ee9b5d0b945c2ccbc1c83467e76c01a857bba3</citedby><cites>FETCH-LOGICAL-c415t-bc79e179c8588d99a5165b54a04ee9b5d0b945c2ccbc1c83467e76c01a857bba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708508003907$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21217924$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18757149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Lei-Chwen</creatorcontrib><creatorcontrib>Chen, Yen-Fei</creatorcontrib><creatorcontrib>Lee, Wen-Chuan</creatorcontrib><creatorcontrib>Wu, Yu-Tse</creatorcontrib><creatorcontrib>Tsai, Tung-Hu</creatorcontrib><title>Pharmacokinetics of gastrodin and its metabolite p-hydroxybenzyl alcohol in rat blood, brain and bile by microdialysis coupled to LC–MS/MS</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>Gastrodin is a pharmacologically active substance isolated from
Gastrodia elata Blume with sedation, anti-convulsion and anti-epilepsy activities. A rapid and sensitive liquid chromatography technique coupled to tandem mass spectrometry (LC–MS/MS) system was developed to determine gastrodin and its metabolite
p-hydroxybenzyl alcohol (HBA) in rat blood, brain and bile collected using microdialysis technique. The analytes were separated using a reversed phase column (4.6
mm
×
150
mm, 5
μm). The mobile phase for column separation was 30% methanol with a flow rate of 0.6
mL/min. As a post-column addition, 1% ammonium hydroxide solution (in methanol) was additionally pumped via a T-connection using a chromatographic pump (BAS PM-80, USA) at a flow rate of 0.2
mL/min after the column separation. A LC–MS/MS system equipped with a negative electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor
m/
z 285.0
→
122.9 and
m/
z 123.0
→
105.0 transitions for gastrodin and HBA, respectively. The lower limit of quantification (LLoQ) for gastrodin and HBA were 0.5 and 2
ng/mL, respectively. The calibration curves were linear over the range of 0.5–5000
ng/mL and 2–1000
ng/mL for gastrodin and HBA with a coefficient of determination >0.995, respectively. This selective and sensitive method is useful for the determination of gastrodin and HBA and in the pharmacokinetic studies of these compounds.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Benzyl Alcohols - blood</subject><subject>Benzyl Alcohols - chemistry</subject><subject>Benzyl Alcohols - isolation & purification</subject><subject>Benzyl Alcohols - metabolism</subject><subject>Benzyl Alcohols - pharmacokinetics</subject><subject>Bile - metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Calibration</subject><subject>Chromatography, Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrodia elata</subject><subject>Gastrodia elata Blume</subject><subject>Gastrodin</subject><subject>General pharmacology</subject><subject>Glucosides - chemistry</subject><subject>Glucosides - isolation & purification</subject><subject>Glucosides - metabolism</subject><subject>Glucosides - pharmacokinetics</subject><subject>Herbal medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microdialysis</subject><subject>Molecular Structure</subject><subject>Pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Roots - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Time Factors</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbGO1DAQhi0E4paDF6BAbqAiOduJ41iiQSsOkPYE0oFEZ9nOLOvFifdsL7pQ8QB0vCFPQqKNoINqmu__NTMfQo8pKSmhzcW-3B-MLhkhbUlESWh1B61oK6qCNfWnu2hFREULQVp-hh6ktCeEcCrr--hsgrigtVyhH-93Ovbahi9ugOxswmGLP-uUY-jcgPXQYZcT7iFrE7zLgA_FbuxiuB0NDN9Gj7W3YRc8nuioMzY-hO45NlEvceM8YDPi3tm5U_sxuYRtOB48dDgHvFn_-v7z6vri6vohurfVPsGjZZ6jj5evPqzfFJt3r9-uX24KW1OeC2OFBCqkbXnbdlJqThtueK1JDSAN74iRNbfMWmOpbau6ESAaS6huuTBGV-fo2an3EMPNEVJWvUsWvNcDhGNSjWwkZYL_F2SENUzSGWQncLoxpQhbdYiu13FUlKhZltqrWZaaZSki1CRrCj1Z2o-mh-5vZLEzAU8XQCer_Tbqwbr0h2OUTV9g9cS9OHEwPe2rg6iSdTBY6FwEm1UX3L_2-A06z7Vm</recordid><startdate>20081104</startdate><enddate>20081104</enddate><creator>Lin, Lei-Chwen</creator><creator>Chen, Yen-Fei</creator><creator>Lee, Wen-Chuan</creator><creator>Wu, Yu-Tse</creator><creator>Tsai, Tung-Hu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20081104</creationdate><title>Pharmacokinetics of gastrodin and its metabolite p-hydroxybenzyl alcohol in rat blood, brain and bile by microdialysis coupled to LC–MS/MS</title><author>Lin, Lei-Chwen ; Chen, Yen-Fei ; Lee, Wen-Chuan ; Wu, Yu-Tse ; Tsai, Tung-Hu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-bc79e179c8588d99a5165b54a04ee9b5d0b945c2ccbc1c83467e76c01a857bba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Benzyl Alcohols - blood</topic><topic>Benzyl Alcohols - chemistry</topic><topic>Benzyl Alcohols - isolation & purification</topic><topic>Benzyl Alcohols - metabolism</topic><topic>Benzyl Alcohols - pharmacokinetics</topic><topic>Bile - metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Calibration</topic><topic>Chromatography, Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrodia elata</topic><topic>Gastrodia elata Blume</topic><topic>Gastrodin</topic><topic>General pharmacology</topic><topic>Glucosides - chemistry</topic><topic>Glucosides - isolation & purification</topic><topic>Glucosides - metabolism</topic><topic>Glucosides - pharmacokinetics</topic><topic>Herbal medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microdialysis</topic><topic>Molecular Structure</topic><topic>Pharmacokinetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Roots - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Lei-Chwen</creatorcontrib><creatorcontrib>Chen, Yen-Fei</creatorcontrib><creatorcontrib>Lee, Wen-Chuan</creatorcontrib><creatorcontrib>Wu, Yu-Tse</creatorcontrib><creatorcontrib>Tsai, Tung-Hu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Lei-Chwen</au><au>Chen, Yen-Fei</au><au>Lee, Wen-Chuan</au><au>Wu, Yu-Tse</au><au>Tsai, Tung-Hu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pharmacokinetics of gastrodin and its metabolite p-hydroxybenzyl alcohol in rat blood, brain and bile by microdialysis coupled to LC–MS/MS</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2008-11-04</date><risdate>2008</risdate><volume>48</volume><issue>3</issue><spage>909</spage><epage>917</epage><pages>909-917</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>Gastrodin is a pharmacologically active substance isolated from
Gastrodia elata Blume with sedation, anti-convulsion and anti-epilepsy activities. A rapid and sensitive liquid chromatography technique coupled to tandem mass spectrometry (LC–MS/MS) system was developed to determine gastrodin and its metabolite
p-hydroxybenzyl alcohol (HBA) in rat blood, brain and bile collected using microdialysis technique. The analytes were separated using a reversed phase column (4.6
mm
×
150
mm, 5
μm). The mobile phase for column separation was 30% methanol with a flow rate of 0.6
mL/min. As a post-column addition, 1% ammonium hydroxide solution (in methanol) was additionally pumped via a T-connection using a chromatographic pump (BAS PM-80, USA) at a flow rate of 0.2
mL/min after the column separation. A LC–MS/MS system equipped with a negative electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor
m/
z 285.0
→
122.9 and
m/
z 123.0
→
105.0 transitions for gastrodin and HBA, respectively. The lower limit of quantification (LLoQ) for gastrodin and HBA were 0.5 and 2
ng/mL, respectively. The calibration curves were linear over the range of 0.5–5000
ng/mL and 2–1000
ng/mL for gastrodin and HBA with a coefficient of determination >0.995, respectively. This selective and sensitive method is useful for the determination of gastrodin and HBA and in the pharmacokinetic studies of these compounds.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18757149</pmid><doi>10.1016/j.jpba.2008.07.013</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2008-11, Vol.48 (3), p.909-917 |
| issn | 0731-7085 1873-264X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis Analytical, structural and metabolic biochemistry Animals Benzyl Alcohols - blood Benzyl Alcohols - chemistry Benzyl Alcohols - isolation & purification Benzyl Alcohols - metabolism Benzyl Alcohols - pharmacokinetics Bile - metabolism Biological and medical sciences Brain - metabolism Calibration Chromatography, Liquid - methods Fundamental and applied biological sciences. Psychology Gastrodia elata Gastrodia elata Blume Gastrodin General pharmacology Glucosides - chemistry Glucosides - isolation & purification Glucosides - metabolism Glucosides - pharmacokinetics Herbal medicine Male Medical sciences Microdialysis Molecular Structure Pharmacokinetics Pharmacology. Drug treatments Plant Roots - chemistry Rats Rats, Sprague-Dawley Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - methods Time Factors |
| title | Pharmacokinetics of gastrodin and its metabolite p-hydroxybenzyl alcohol in rat blood, brain and bile by microdialysis coupled to LC–MS/MS |
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