Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD–MS
An HPLC-DAD–MS method was developed for simultaneous determination of the five major active constituents in Erigeron multiradiatus (Wall.) Benth, namely 6′- O-cafferylerigeroside (1), scutellarin (2), apigenin-7- O-β- d-glucuronide (3), apigenin (4) and kaempferol (5), respectively. They were identi...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2008-11, Vol.48 (3), p.980-985 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 985 |
|---|---|
| container_issue | 3 |
| container_start_page | 980 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 48 |
| creator | Zhang, Zhifeng Sun, Wenxia Luo, Pei Wu, Liping Ye, Limin Zhang, Hao |
| description | An HPLC-DAD–MS method was developed for simultaneous determination of the five major active constituents in
Erigeron multiradiatus (Wall.) Benth, namely 6′-
O-cafferylerigeroside (1), scutellarin (2), apigenin-7-
O-β-
d-glucuronide (3), apigenin (4) and kaempferol (5), respectively. They were identified by ESI-MS and comparisons with literature. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with
r
>
0.999. The precision was evaluated by intra- and inter-day assays, and relative standard deviation (R.S.D.) values were reported within 2.7%. The recovery studies for the quantified compounds were observed in the range of 95.3–102.4% with R.S.D. values less than 2.3%. The overall procedure may be suitable for the qualitative and quantitative analyses of a large number of
E. multiradiatus samples. Hierarchical clustering analysis based on the characteristics of the 5 investigated compound peaks in HPLC profiles showed that 18 samples were divided into 2 main clusters. The clusters corresponded to their content. The five constituents in
E. multiradiatus are generally regarded as an index for the quality assessment of this herb. |
| doi_str_mv | 10.1016/j.jpba.2008.06.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69690606</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708508003555</els_id><sourcerecordid>69690606</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-4fa7e5879b3c1600825f9048fde76341385a5db81008825f6c6788be8c3077f63</originalsourceid><addsrcrecordid>eNp9kM1u1DAURi0EokPhBVigbOgu4TpObEdiU01LizQIpILEznKca-RRfgbbqdQd78Ab8iS1NSPYsbKle-5nf4eQ1xQqCpS_21f7Q6-rGkBWwCug8IRsqBSsrHnz_SnZgGC0FCDbM_IihD0AtLRrnpMzKrmQTdtuiL1z0zpGPeOyhmLAiH5ys45umYvFFtbdYzFpNxfaxHw3yxyiiyvOMWTg2rsf6BOcU5zXg9MxBfUPxe2X3ba8urz68-v3p7uX5JnVY8BXp_OcfPtw_XV7W-4-33zcXu5Kw2QTy8Zqga0UXc8M5alX3doOGmkHFJw1lMlWt0MvaRrlGTeph-xRGgZCWM7OycUx9-CXnyuGqCYXDI7jsaDiHe-AQwbrI2j8EoJHqw7eTdo_KAoq21V7le2qbFcBV8luWnpzSl_7CYd_KyedCXh7AnQwerRez8aFv1xNayo6ll9_f-Qwubh36FUwDmeDg_NoohoW979_PAKb3ZkD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69690606</pqid></control><display><type>article</type><title>Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD–MS</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Zhang, Zhifeng ; Sun, Wenxia ; Luo, Pei ; Wu, Liping ; Ye, Limin ; Zhang, Hao</creator><creatorcontrib>Zhang, Zhifeng ; Sun, Wenxia ; Luo, Pei ; Wu, Liping ; Ye, Limin ; Zhang, Hao</creatorcontrib><description>An HPLC-DAD–MS method was developed for simultaneous determination of the five major active constituents in
Erigeron multiradiatus (Wall.) Benth, namely 6′-
O-cafferylerigeroside (1), scutellarin (2), apigenin-7-
O-β-
d-glucuronide (3), apigenin (4) and kaempferol (5), respectively. They were identified by ESI-MS and comparisons with literature. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with
r
>
0.999. The precision was evaluated by intra- and inter-day assays, and relative standard deviation (R.S.D.) values were reported within 2.7%. The recovery studies for the quantified compounds were observed in the range of 95.3–102.4% with R.S.D. values less than 2.3%. The overall procedure may be suitable for the qualitative and quantitative analyses of a large number of
E. multiradiatus samples. Hierarchical clustering analysis based on the characteristics of the 5 investigated compound peaks in HPLC profiles showed that 18 samples were divided into 2 main clusters. The clusters corresponded to their content. The five constituents in
E. multiradiatus are generally regarded as an index for the quality assessment of this herb.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2008.06.010</identifier><identifier>PMID: 18678455</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Apigenin - analysis ; Apigenin - chemistry ; Biological and medical sciences ; Calibration ; Chromatography, High Pressure Liquid - methods ; Cluster Analysis ; Erigeron - chemistry ; Erigeron multiradiatus ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Glucuronates - analysis ; Glucuronates - chemistry ; Hot Temperature ; HPLC-DAD–MS ; Kaempferols - analysis ; Kaempferols - chemistry ; Linear Models ; Mass Spectrometry - methods ; Medical sciences ; Molecular Structure ; Pharmacology. Drug treatments ; Plant Extracts - chemistry ; Pyrones - analysis ; Pyrones - chemistry ; Quality assessment ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods ; Time Factors</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2008-11, Vol.48 (3), p.980-985</ispartof><rights>2008</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-4fa7e5879b3c1600825f9048fde76341385a5db81008825f6c6788be8c3077f63</citedby><cites>FETCH-LOGICAL-c384t-4fa7e5879b3c1600825f9048fde76341385a5db81008825f6c6788be8c3077f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2008.06.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21217936$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18678455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Zhifeng</creatorcontrib><creatorcontrib>Sun, Wenxia</creatorcontrib><creatorcontrib>Luo, Pei</creatorcontrib><creatorcontrib>Wu, Liping</creatorcontrib><creatorcontrib>Ye, Limin</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><title>Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD–MS</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>An HPLC-DAD–MS method was developed for simultaneous determination of the five major active constituents in
Erigeron multiradiatus (Wall.) Benth, namely 6′-
O-cafferylerigeroside (1), scutellarin (2), apigenin-7-
O-β-
d-glucuronide (3), apigenin (4) and kaempferol (5), respectively. They were identified by ESI-MS and comparisons with literature. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with
r
>
0.999. The precision was evaluated by intra- and inter-day assays, and relative standard deviation (R.S.D.) values were reported within 2.7%. The recovery studies for the quantified compounds were observed in the range of 95.3–102.4% with R.S.D. values less than 2.3%. The overall procedure may be suitable for the qualitative and quantitative analyses of a large number of
E. multiradiatus samples. Hierarchical clustering analysis based on the characteristics of the 5 investigated compound peaks in HPLC profiles showed that 18 samples were divided into 2 main clusters. The clusters corresponded to their content. The five constituents in
E. multiradiatus are generally regarded as an index for the quality assessment of this herb.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Apigenin - analysis</subject><subject>Apigenin - chemistry</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cluster Analysis</subject><subject>Erigeron - chemistry</subject><subject>Erigeron multiradiatus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Glucuronates - analysis</subject><subject>Glucuronates - chemistry</subject><subject>Hot Temperature</subject><subject>HPLC-DAD–MS</subject><subject>Kaempferols - analysis</subject><subject>Kaempferols - chemistry</subject><subject>Linear Models</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - chemistry</subject><subject>Pyrones - analysis</subject><subject>Pyrones - chemistry</subject><subject>Quality assessment</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Time Factors</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAURi0EokPhBVigbOgu4TpObEdiU01LizQIpILEznKca-RRfgbbqdQd78Ab8iS1NSPYsbKle-5nf4eQ1xQqCpS_21f7Q6-rGkBWwCug8IRsqBSsrHnz_SnZgGC0FCDbM_IihD0AtLRrnpMzKrmQTdtuiL1z0zpGPeOyhmLAiH5ys45umYvFFtbdYzFpNxfaxHw3yxyiiyvOMWTg2rsf6BOcU5zXg9MxBfUPxe2X3ba8urz68-v3p7uX5JnVY8BXp_OcfPtw_XV7W-4-33zcXu5Kw2QTy8Zqga0UXc8M5alX3doOGmkHFJw1lMlWt0MvaRrlGTeph-xRGgZCWM7OycUx9-CXnyuGqCYXDI7jsaDiHe-AQwbrI2j8EoJHqw7eTdo_KAoq21V7le2qbFcBV8luWnpzSl_7CYd_KyedCXh7AnQwerRez8aFv1xNayo6ll9_f-Qwubh36FUwDmeDg_NoohoW979_PAKb3ZkD</recordid><startdate>20081104</startdate><enddate>20081104</enddate><creator>Zhang, Zhifeng</creator><creator>Sun, Wenxia</creator><creator>Luo, Pei</creator><creator>Wu, Liping</creator><creator>Ye, Limin</creator><creator>Zhang, Hao</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081104</creationdate><title>Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD–MS</title><author>Zhang, Zhifeng ; Sun, Wenxia ; Luo, Pei ; Wu, Liping ; Ye, Limin ; Zhang, Hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-4fa7e5879b3c1600825f9048fde76341385a5db81008825f6c6788be8c3077f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Apigenin - analysis</topic><topic>Apigenin - chemistry</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cluster Analysis</topic><topic>Erigeron - chemistry</topic><topic>Erigeron multiradiatus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Glucuronates - analysis</topic><topic>Glucuronates - chemistry</topic><topic>Hot Temperature</topic><topic>HPLC-DAD–MS</topic><topic>Kaempferols - analysis</topic><topic>Kaempferols - chemistry</topic><topic>Linear Models</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Molecular Structure</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - chemistry</topic><topic>Pyrones - analysis</topic><topic>Pyrones - chemistry</topic><topic>Quality assessment</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhifeng</creatorcontrib><creatorcontrib>Sun, Wenxia</creatorcontrib><creatorcontrib>Luo, Pei</creatorcontrib><creatorcontrib>Wu, Liping</creatorcontrib><creatorcontrib>Ye, Limin</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhifeng</au><au>Sun, Wenxia</au><au>Luo, Pei</au><au>Wu, Liping</au><au>Ye, Limin</au><au>Zhang, Hao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD–MS</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2008-11-04</date><risdate>2008</risdate><volume>48</volume><issue>3</issue><spage>980</spage><epage>985</epage><pages>980-985</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>An HPLC-DAD–MS method was developed for simultaneous determination of the five major active constituents in
Erigeron multiradiatus (Wall.) Benth, namely 6′-
O-cafferylerigeroside (1), scutellarin (2), apigenin-7-
O-β-
d-glucuronide (3), apigenin (4) and kaempferol (5), respectively. They were identified by ESI-MS and comparisons with literature. A comprehensive validation of the method included tests of sensitivity, linearity, precision and accuracy. The linear regressions were acquired with
r
>
0.999. The precision was evaluated by intra- and inter-day assays, and relative standard deviation (R.S.D.) values were reported within 2.7%. The recovery studies for the quantified compounds were observed in the range of 95.3–102.4% with R.S.D. values less than 2.3%. The overall procedure may be suitable for the qualitative and quantitative analyses of a large number of
E. multiradiatus samples. Hierarchical clustering analysis based on the characteristics of the 5 investigated compound peaks in HPLC profiles showed that 18 samples were divided into 2 main clusters. The clusters corresponded to their content. The five constituents in
E. multiradiatus are generally regarded as an index for the quality assessment of this herb.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18678455</pmid><doi>10.1016/j.jpba.2008.06.010</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2008-11, Vol.48 (3), p.980-985 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69690606 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analysis Analytical, structural and metabolic biochemistry Apigenin - analysis Apigenin - chemistry Biological and medical sciences Calibration Chromatography, High Pressure Liquid - methods Cluster Analysis Erigeron - chemistry Erigeron multiradiatus Fundamental and applied biological sciences. Psychology General pharmacology Glucuronates - analysis Glucuronates - chemistry Hot Temperature HPLC-DAD–MS Kaempferols - analysis Kaempferols - chemistry Linear Models Mass Spectrometry - methods Medical sciences Molecular Structure Pharmacology. Drug treatments Plant Extracts - chemistry Pyrones - analysis Pyrones - chemistry Quality assessment Reference Standards Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Time Factors |
| title | Simultaneous determination of five main active constituents of Erigeron multiradiatus by HPLC-DAD–MS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A22%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20determination%20of%20five%20main%20active%20constituents%20of%20Erigeron%20multiradiatus%20by%20HPLC-DAD%E2%80%93MS&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Zhang,%20Zhifeng&rft.date=2008-11-04&rft.volume=48&rft.issue=3&rft.spage=980&rft.epage=985&rft.pages=980-985&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/j.jpba.2008.06.010&rft_dat=%3Cproquest_cross%3E69690606%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69690606&rft_id=info:pmid/18678455&rft_els_id=S0731708508003555&rfr_iscdi=true |