Are there long-term changes in the basal or evoked Fos expression in the dorsal horn of the spinal cord of the mononeuropathic rat?
The long-term changes in Fos like-immunoreactivity (Fos-LI) in the dorsal horn of the spinal cord following various peripheral nerve lesions remain controversial. This study considers such an approach with chronic constriction injury rats (CCI: loose ligations of the sciatic nerve), at 2 weeks after...
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| Veröffentlicht in: | Pain (Amsterdam) 1999-03, Vol.80 (1), p.347-357 |
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| description | The long-term changes in Fos like-immunoreactivity (Fos-LI) in the dorsal horn of the spinal cord following various peripheral nerve lesions remain controversial. This study considers such an approach with chronic constriction injury rats (CCI: loose ligations of the sciatic nerve), at 2 weeks after the surgery, when changes in spontaneous and evoked behaviour were clearly described. All rats used for Fos studies displayed allodynia to mechanical stimulation (decrease of 32% of the vocalization threshold to paw pressure). In CCI rats, which displayed `spontaneous pain-related behaviour', the number of Fos-LI neurones, in the absence of any intentional stimulation, was very low and comparable with that observed in normal and sham-operated rats ( |
| doi_str_mv | 10.1016/S0304-3959(98)00234-6 |
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μm section). Thus, in this model, the expression of Fos protein is not a reliable index of spontaneous pain. Surprisingly, despite the fact that in this model numerous anatomical studies described a dramatic loss of large and unmyelinated primary afferent fibers, we were unable to detect changes in the number and distribution of Fos-LI evoked by various modalities of peripheral noxious stimulation (noxious thermal stimuli, noxious mechanical stimuli and carrageenin induced inflammation). For example, the stimulus-response curves for the number of Fos-LI neurones evoked by a series of heat stimuli (40, 45, 48, 52, 55°C) were almost superimposable for CCI, sham-operated and normal rats. In contrast, stroking of the nerve-injured paw induced a significant expression of Fos-LI in the superficial laminae (I–II) of the dorsal horn of CCI rats (19.5±3/sections,
P=0.027) which was greater than that observed in sham-operated (6.5±3/sections) or in normal rats (3.5±2/section). These modifications may reflect mechanical allodynia observed in behavioural studies and could be related to A
β fibers, which are known to be severely affected after the constriction of the nerve. These results suggest that this approach could be useful to study, at the cellular level, in freely moving rats, some pharmacological aspects of neuropathic pain.</description><identifier>ISSN: 0304-3959</identifier><identifier>EISSN: 1872-6623</identifier><identifier>DOI: 10.1016/S0304-3959(98)00234-6</identifier><identifier>PMID: 10204748</identifier><identifier>CODEN: PAINDB</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Allodynia ; Animals ; Biological and medical sciences ; Carrageenan ; Chronic constriction injury ; Chronic Disease ; Cold Temperature ; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction ; Fos protein ; Hot Temperature ; Hyperalgesia ; Immunohistochemistry ; Ligation ; Male ; Medical sciences ; Nervous system (semeiology, syndromes) ; Neurology ; Neurons - drug effects ; Neuropathic pain ; Pain Measurement ; Peripheral Nervous System Diseases - genetics ; Peripheral Nervous System Diseases - metabolism ; Physical Stimulation ; Proto-Oncogene Proteins c-fos - metabolism ; Rats ; Rats, Sprague-Dawley ; Sciatic Nerve ; Spinal cord ; Spinal Cord - metabolism ; Spinal Cord - pathology ; Stimulation, Chemical</subject><ispartof>Pain (Amsterdam), 1999-03, Vol.80 (1), p.347-357</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-7debf1e8197e5f7b78231549dea910f2606797774ee88ea54a5375677ee4cb4e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304395998002346$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1781089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10204748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Catheline, Gwénaëlle</creatorcontrib><creatorcontrib>Le Guen, Stéphanie</creatorcontrib><creatorcontrib>Honoré, Prisca</creatorcontrib><creatorcontrib>Besson, Jean-Marie</creatorcontrib><title>Are there long-term changes in the basal or evoked Fos expression in the dorsal horn of the spinal cord of the mononeuropathic rat?</title><title>Pain (Amsterdam)</title><addtitle>Pain</addtitle><description>The long-term changes in Fos like-immunoreactivity (Fos-LI) in the dorsal horn of the spinal cord following various peripheral nerve lesions remain controversial. This study considers such an approach with chronic constriction injury rats (CCI: loose ligations of the sciatic nerve), at 2 weeks after the surgery, when changes in spontaneous and evoked behaviour were clearly described. All rats used for Fos studies displayed allodynia to mechanical stimulation (decrease of 32% of the vocalization threshold to paw pressure). In CCI rats, which displayed `spontaneous pain-related behaviour', the number of Fos-LI neurones, in the absence of any intentional stimulation, was very low and comparable with that observed in normal and sham-operated rats (<10 neurones/40
μm section). Thus, in this model, the expression of Fos protein is not a reliable index of spontaneous pain. Surprisingly, despite the fact that in this model numerous anatomical studies described a dramatic loss of large and unmyelinated primary afferent fibers, we were unable to detect changes in the number and distribution of Fos-LI evoked by various modalities of peripheral noxious stimulation (noxious thermal stimuli, noxious mechanical stimuli and carrageenin induced inflammation). For example, the stimulus-response curves for the number of Fos-LI neurones evoked by a series of heat stimuli (40, 45, 48, 52, 55°C) were almost superimposable for CCI, sham-operated and normal rats. In contrast, stroking of the nerve-injured paw induced a significant expression of Fos-LI in the superficial laminae (I–II) of the dorsal horn of CCI rats (19.5±3/sections,
P=0.027) which was greater than that observed in sham-operated (6.5±3/sections) or in normal rats (3.5±2/section). These modifications may reflect mechanical allodynia observed in behavioural studies and could be related to A
β fibers, which are known to be severely affected after the constriction of the nerve. These results suggest that this approach could be useful to study, at the cellular level, in freely moving rats, some pharmacological aspects of neuropathic pain.</description><subject>Allodynia</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carrageenan</subject><subject>Chronic constriction injury</subject><subject>Chronic Disease</subject><subject>Cold Temperature</subject><subject>Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction</subject><subject>Fos protein</subject><subject>Hot Temperature</subject><subject>Hyperalgesia</subject><subject>Immunohistochemistry</subject><subject>Ligation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Neurons - drug effects</subject><subject>Neuropathic pain</subject><subject>Pain Measurement</subject><subject>Peripheral Nervous System Diseases - genetics</subject><subject>Peripheral Nervous System Diseases - metabolism</subject><subject>Physical Stimulation</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sciatic Nerve</subject><subject>Spinal cord</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal Cord - pathology</subject><subject>Stimulation, Chemical</subject><issn>0304-3959</issn><issn>1872-6623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtP3TAQRq2qqFygP6GVF1UFi4AdO36sEEI8KiGxaLu2HGfCdZvYqZ2L6Lp_vL4PaHdsZqTPZ2asg9AHSk4poeLsK2GEV0w3-lirE0JqxivxBi2oknUlRM3eosULso8Ocv5BClXX-h3ap6QmXHK1QH8uEuB5CaUOMTxUM6QRu6UND5CxD-sn3NpsBxwThsf4Ezp8HTOGpylBzj6GZ6qLaY0tYwo49psoTz6UyMXUPUdjDDHAKsXJzkvvcLLz-RHa6-2Q4f2uH6Lv11ffLm-ru_ubL5cXd5VjmsyV7KDtKSiqJTS9bKWqGW247sBqSvpaECG1lJIDKAW24bZhshFSAnDXcmCH6PN275TirxXk2Yw-OxgGGyCushFaqLKTFbDZgi7FnBP0Zkp-tOm3ocSs7ZuNfbNWa7QyG_tGlLmPuwOrdoTuv6mt7gJ82gE2Ozv0yQbn8z9OKkqULtj5FoNi49FDMtl5CA46n8DNpov-lZ_8BQHNoeI</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>Catheline, Gwénaëlle</creator><creator>Le Guen, Stéphanie</creator><creator>Honoré, Prisca</creator><creator>Besson, Jean-Marie</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990301</creationdate><title>Are there long-term changes in the basal or evoked Fos expression in the dorsal horn of the spinal cord of the mononeuropathic rat?</title><author>Catheline, Gwénaëlle ; Le Guen, Stéphanie ; Honoré, Prisca ; Besson, Jean-Marie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-7debf1e8197e5f7b78231549dea910f2606797774ee88ea54a5375677ee4cb4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Allodynia</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carrageenan</topic><topic>Chronic constriction injury</topic><topic>Chronic Disease</topic><topic>Cold Temperature</topic><topic>Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction</topic><topic>Fos protein</topic><topic>Hot Temperature</topic><topic>Hyperalgesia</topic><topic>Immunohistochemistry</topic><topic>Ligation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Neurons - drug effects</topic><topic>Neuropathic pain</topic><topic>Pain Measurement</topic><topic>Peripheral Nervous System Diseases - genetics</topic><topic>Peripheral Nervous System Diseases - metabolism</topic><topic>Physical Stimulation</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sciatic Nerve</topic><topic>Spinal cord</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal Cord - pathology</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Catheline, Gwénaëlle</creatorcontrib><creatorcontrib>Le Guen, Stéphanie</creatorcontrib><creatorcontrib>Honoré, Prisca</creatorcontrib><creatorcontrib>Besson, Jean-Marie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pain (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Catheline, Gwénaëlle</au><au>Le Guen, Stéphanie</au><au>Honoré, Prisca</au><au>Besson, Jean-Marie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Are there long-term changes in the basal or evoked Fos expression in the dorsal horn of the spinal cord of the mononeuropathic rat?</atitle><jtitle>Pain (Amsterdam)</jtitle><addtitle>Pain</addtitle><date>1999-03-01</date><risdate>1999</risdate><volume>80</volume><issue>1</issue><spage>347</spage><epage>357</epage><pages>347-357</pages><issn>0304-3959</issn><eissn>1872-6623</eissn><coden>PAINDB</coden><abstract>The long-term changes in Fos like-immunoreactivity (Fos-LI) in the dorsal horn of the spinal cord following various peripheral nerve lesions remain controversial. This study considers such an approach with chronic constriction injury rats (CCI: loose ligations of the sciatic nerve), at 2 weeks after the surgery, when changes in spontaneous and evoked behaviour were clearly described. All rats used for Fos studies displayed allodynia to mechanical stimulation (decrease of 32% of the vocalization threshold to paw pressure). In CCI rats, which displayed `spontaneous pain-related behaviour', the number of Fos-LI neurones, in the absence of any intentional stimulation, was very low and comparable with that observed in normal and sham-operated rats (<10 neurones/40
μm section). Thus, in this model, the expression of Fos protein is not a reliable index of spontaneous pain. Surprisingly, despite the fact that in this model numerous anatomical studies described a dramatic loss of large and unmyelinated primary afferent fibers, we were unable to detect changes in the number and distribution of Fos-LI evoked by various modalities of peripheral noxious stimulation (noxious thermal stimuli, noxious mechanical stimuli and carrageenin induced inflammation). For example, the stimulus-response curves for the number of Fos-LI neurones evoked by a series of heat stimuli (40, 45, 48, 52, 55°C) were almost superimposable for CCI, sham-operated and normal rats. In contrast, stroking of the nerve-injured paw induced a significant expression of Fos-LI in the superficial laminae (I–II) of the dorsal horn of CCI rats (19.5±3/sections,
P=0.027) which was greater than that observed in sham-operated (6.5±3/sections) or in normal rats (3.5±2/section). These modifications may reflect mechanical allodynia observed in behavioural studies and could be related to A
β fibers, which are known to be severely affected after the constriction of the nerve. These results suggest that this approach could be useful to study, at the cellular level, in freely moving rats, some pharmacological aspects of neuropathic pain.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10204748</pmid><doi>10.1016/S0304-3959(98)00234-6</doi><tpages>11</tpages></addata></record> |
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| ispartof | Pain (Amsterdam), 1999-03, Vol.80 (1), p.347-357 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Allodynia Animals Biological and medical sciences Carrageenan Chronic constriction injury Chronic Disease Cold Temperature Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction Fos protein Hot Temperature Hyperalgesia Immunohistochemistry Ligation Male Medical sciences Nervous system (semeiology, syndromes) Neurology Neurons - drug effects Neuropathic pain Pain Measurement Peripheral Nervous System Diseases - genetics Peripheral Nervous System Diseases - metabolism Physical Stimulation Proto-Oncogene Proteins c-fos - metabolism Rats Rats, Sprague-Dawley Sciatic Nerve Spinal cord Spinal Cord - metabolism Spinal Cord - pathology Stimulation, Chemical |
| title | Are there long-term changes in the basal or evoked Fos expression in the dorsal horn of the spinal cord of the mononeuropathic rat? |
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