The CC chemokine receptor-7 ligands 6Ckine and macrophage inflammatory protein-3 beta are potent chemoattractants for in vitro- and in vivo-derived dendritic cells
Dendritic cell migration to secondary lymphoid tissues is critical for Ag presentation to T cells necessary to elicit an immune response. Despite the importance of dendritic cell trafficking in immunity, at present little is understood about the mechanisms that underlie this phenomenon. Using a nove...
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| Veröffentlicht in: | The Journal of immunology (1950) 1999-04, Vol.162 (7), p.3859-3864 |
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| container_title | The Journal of immunology (1950) |
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| creator | Kellermann, S A Hudak, S Oldham, E R Liu, Y J McEvoy, L M |
| description | Dendritic cell migration to secondary lymphoid tissues is critical for Ag presentation to T cells necessary to elicit an immune response. Despite the importance of dendritic cell trafficking in immunity, at present little is understood about the mechanisms that underlie this phenomenon. Using a novel transwell chemotaxis assay system, we demonstrate that the CC chemokine receptor-7 (CCR7) ligands 6Ckine and macrophage inflammatory protein (MIP)-3 beta are selective chemoattractants for MHC class IIhigh B7-2high bone marrow-derived dendritic cells at a potency 1000-fold higher than their known activity on naive T cells. Furthermore, these chemokines stimulate the chemotaxis of freshly isolated lymph node dendritic cells, as well as the egress of skin dendritic cells ex vivo. Because these chemokines are expressed in lymphoid organs and 6Ckine has been localized to high endothelial venules and lymphatic endothelium, we propose that they may play an important role in the homing of dendritic cells to lymphoid tissues. |
| doi_str_mv | 10.4049/jimmunol.162.7.3859 |
| format | Article |
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Hudak, S ; Oldham, E R ; Liu, Y J ; McEvoy, L M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-789e5cd17c75dc9b59c703996008639f92f9d6aad97ad27349f60e60b2f43fa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Bone Marrow Cells</topic><topic>Cells, Cultured</topic><topic>Chemokine CCL19</topic><topic>Chemokine CCL21</topic><topic>Chemokine CXCL12</topic><topic>Chemokines, CC - physiology</topic><topic>Chemokines, CXC - physiology</topic><topic>Chemotaxis - immunology</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - metabolism</topic><topic>Female</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Receptors, CCR7</topic><topic>Receptors, Chemokine - genetics</topic><topic>Receptors, Chemokine - metabolism</topic><topic>Skin - cytology</topic><topic>Skin - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kellermann, S A</creatorcontrib><creatorcontrib>Hudak, S</creatorcontrib><creatorcontrib>Oldham, E R</creatorcontrib><creatorcontrib>Liu, Y J</creatorcontrib><creatorcontrib>McEvoy, L M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kellermann, S A</au><au>Hudak, S</au><au>Oldham, E R</au><au>Liu, Y J</au><au>McEvoy, L M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The CC chemokine receptor-7 ligands 6Ckine and macrophage inflammatory protein-3 beta are potent chemoattractants for in vitro- and in vivo-derived dendritic cells</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1999-04-01</date><risdate>1999</risdate><volume>162</volume><issue>7</issue><spage>3859</spage><epage>3864</epage><pages>3859-3864</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Dendritic cell migration to secondary lymphoid tissues is critical for Ag presentation to T cells necessary to elicit an immune response. Despite the importance of dendritic cell trafficking in immunity, at present little is understood about the mechanisms that underlie this phenomenon. Using a novel transwell chemotaxis assay system, we demonstrate that the CC chemokine receptor-7 (CCR7) ligands 6Ckine and macrophage inflammatory protein (MIP)-3 beta are selective chemoattractants for MHC class IIhigh B7-2high bone marrow-derived dendritic cells at a potency 1000-fold higher than their known activity on naive T cells. Furthermore, these chemokines stimulate the chemotaxis of freshly isolated lymph node dendritic cells, as well as the egress of skin dendritic cells ex vivo. 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bone Marrow Cells Cells, Cultured Chemokine CCL19 Chemokine CCL21 Chemokine CXCL12 Chemokines, CC - physiology Chemokines, CXC - physiology Chemotaxis - immunology Dendritic Cells - cytology Dendritic Cells - immunology Dendritic Cells - metabolism Female Lymph Nodes - cytology Lymph Nodes - immunology Mice Mice, Inbred BALB C Receptors, CCR7 Receptors, Chemokine - genetics Receptors, Chemokine - metabolism Skin - cytology Skin - immunology |
| title | The CC chemokine receptor-7 ligands 6Ckine and macrophage inflammatory protein-3 beta are potent chemoattractants for in vitro- and in vivo-derived dendritic cells |
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