A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm

An immunocapture (IC) One-step RT-PCR assay was developed to improve the detection of Banana bract mosaic virus (BBrMV) in single and bulked samples of banana plants. In this paper, an atypical strain of BBrMV was described, the BBrMV “Ref” strain, and we showed that detection with available BBrMV t...

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Veröffentlicht in:	Journal of virological methods 2008-11, Vol.153 (2), p.223-231
Hauptverfasser:	Iskra-Caruana, Marie-line, Galzi, Serge, Laboureau, Nathalie
Format:	Artikel
Sprache:	eng
Schlagworte:	Banana bract mosaic virus Biological and medical sciences Bulked detection DNA Primers ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology IC One-step RT-PCR Microbiology Musa Musa - virology Musa germplasm Plant Diseases - virology Plant Leaves - virology Potyvirus - classification Potyvirus - genetics Potyvirus - isolation & purification Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis RNA, Viral - isolation & purification Sensitivity and Specificity Techniques used in virology Virology
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