The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiology
Antiporters exporting Na+ and K+ in exchange for protons are conserved among yeast species. The only exception so far has been Zygosaccharomyces rouxii, an osmotolerant species closely related to Saccharomyces cerevisiae. Z. rouxii was described as possessing one plasma-membrane antiporter transport...
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| Veröffentlicht in: | Fungal genetics and biology 2008-10, Vol.45 (10), p.1439-1447 |
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| creator | Pribylova, Lenka Papouskova, Klara Sychrova, Hana |
| description | Antiporters exporting Na+ and K+ in exchange for protons are conserved among yeast species. The only exception so far has been Zygosaccharomyces rouxii, an osmotolerant species closely related to Saccharomyces cerevisiae. Z. rouxii was described as possessing one plasma-membrane antiporter transporting only Na+ (ZrSod2–22p in the CBS 732T type strain). We report the characterization of a second gene, ZrNHA1, encoding a new K+(Na+)/H+-antiporter capable of both K+ and Na+ export. Synteny analyses suggested that ZrSOD2–22 originated by single duplication of the ZrNHA1 gene. Substrate specificities and transport properties of ZrNha1p and ZrSod2–22p were compared upon heterologous expression in S. cerevisiae, and then directly in Z. rouxii. Deletion mutants and phenotype analyses revealed that ZrSod2–22 antiporter is important for Na+ detoxification, probably together with ZrEna1 ATPase; ZrNha1p is indispensable to maintain potassium homeostasis and ZrEna1p is not, in contrast to the situation in S. cerevisiae, involved in this function. |
| doi_str_mv | 10.1016/j.fgb.2008.08.001 |
| format | Article |
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The only exception so far has been Zygosaccharomyces rouxii, an osmotolerant species closely related to Saccharomyces cerevisiae. Z. rouxii was described as possessing one plasma-membrane antiporter transporting only Na+ (ZrSod2–22p in the CBS 732T type strain). We report the characterization of a second gene, ZrNHA1, encoding a new K+(Na+)/H+-antiporter capable of both K+ and Na+ export. Synteny analyses suggested that ZrSOD2–22 originated by single duplication of the ZrNHA1 gene. Substrate specificities and transport properties of ZrNha1p and ZrSod2–22p were compared upon heterologous expression in S. cerevisiae, and then directly in Z. rouxii. Deletion mutants and phenotype analyses revealed that ZrSod2–22 antiporter is important for Na+ detoxification, probably together with ZrEna1 ATPase; ZrNha1p is indispensable to maintain potassium homeostasis and ZrEna1p is not, in contrast to the situation in S. cerevisiae, involved in this function.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2008.08.001</identifier><identifier>PMID: 18761413</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alkali-metal-cation transporters ; Amino Acid Sequence ; antiporters ; cations ; Cations - metabolism ; cell physiology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; fungus physiology ; Gene Expression ; genes ; Homeostasis ; ion transport ; microbial genetics ; Molecular Sequence Data ; mutants ; Na+/H+-antiporter ; osmotolerance ; phenotype ; plasma membrane ; potassium ; Potassium homeostasis ; Salt tolerance ; Sequence Alignment ; Sequence Deletion ; sodium ; Sodium Chloride - metabolism ; Sodium-Hydrogen Exchangers - genetics ; Sodium-Hydrogen Exchangers - metabolism ; strain differences ; ZrNHA1 gene ; Zygosaccharomyces ; Zygosaccharomyces - genetics ; Zygosaccharomyces - physiology ; Zygosaccharomyces rouxii</subject><ispartof>Fungal genetics and biology, 2008-10, Vol.45 (10), p.1439-1447</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-7e5cd887a4469cb1c30ff5ccfdf1a59ec5cb4e00a52b74ce7a31d4fa6dfc84ab3</citedby><cites>FETCH-LOGICAL-c375t-7e5cd887a4469cb1c30ff5ccfdf1a59ec5cb4e00a52b74ce7a31d4fa6dfc84ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fgb.2008.08.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18761413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pribylova, Lenka</creatorcontrib><creatorcontrib>Papouskova, Klara</creatorcontrib><creatorcontrib>Sychrova, Hana</creatorcontrib><title>The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiology</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>Antiporters exporting Na+ and K+ in exchange for protons are conserved among yeast species. The only exception so far has been Zygosaccharomyces rouxii, an osmotolerant species closely related to Saccharomyces cerevisiae. Z. rouxii was described as possessing one plasma-membrane antiporter transporting only Na+ (ZrSod2–22p in the CBS 732T type strain). We report the characterization of a second gene, ZrNHA1, encoding a new K+(Na+)/H+-antiporter capable of both K+ and Na+ export. Synteny analyses suggested that ZrSOD2–22 originated by single duplication of the ZrNHA1 gene. Substrate specificities and transport properties of ZrNha1p and ZrSod2–22p were compared upon heterologous expression in S. cerevisiae, and then directly in Z. rouxii. Deletion mutants and phenotype analyses revealed that ZrSod2–22 antiporter is important for Na+ detoxification, probably together with ZrEna1 ATPase; ZrNha1p is indispensable to maintain potassium homeostasis and ZrEna1p is not, in contrast to the situation in S. cerevisiae, involved in this function.</description><subject>Alkali-metal-cation transporters</subject><subject>Amino Acid Sequence</subject><subject>antiporters</subject><subject>cations</subject><subject>Cations - metabolism</subject><subject>cell physiology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>fungus physiology</subject><subject>Gene Expression</subject><subject>genes</subject><subject>Homeostasis</subject><subject>ion transport</subject><subject>microbial genetics</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>Na+/H+-antiporter</subject><subject>osmotolerance</subject><subject>phenotype</subject><subject>plasma membrane</subject><subject>potassium</subject><subject>Potassium homeostasis</subject><subject>Salt tolerance</subject><subject>Sequence Alignment</subject><subject>Sequence Deletion</subject><subject>sodium</subject><subject>Sodium Chloride - metabolism</subject><subject>Sodium-Hydrogen Exchangers - genetics</subject><subject>Sodium-Hydrogen Exchangers - metabolism</subject><subject>strain differences</subject><subject>ZrNHA1 gene</subject><subject>Zygosaccharomyces</subject><subject>Zygosaccharomyces - genetics</subject><subject>Zygosaccharomyces - physiology</subject><subject>Zygosaccharomyces rouxii</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEoqXwAGzAKwSqMrXzH7FCFVCkqizabmZj3TjXiUdJHHw9QHa8A-_Dw_AkOMxI7JCuZC--c3x8TxQ9F3wjuCgudhvdNZuE82qzDhcPolPB6yLmdVo-XO9VGYsqy0-iJ0S7AIg8E4-jE1GVhchEehr9uuuREQyeeTugg8mzBYE82y6dJVCqB2fHRSExZ_ffjWGzJcJ1mP9m2TwAjRCPODZBjOwGzi-uzuPgY2brPDpir7fupgcxM5hatnW3tk1-__iZJPObVb2YqWOt0RodhsddSEHMTEyBN3ZivR3Rkgcy9FevcBjY3C9k7GC75Wn0SMNA-Ox4nkX3H97fXV7F158_frp8dx2rtMx9XGKu2qoqIcuKWjVCpVzrXCndagF5jSpXTYacQ540ZaawhFS0mYai1arKoEnPolcH39nZL3skL0dDa5bwZ7snWdRFWdclD6A4gMqFPTnUcnZmBLdIweVamtzJUJpcS5PrcBE0L47m-2bE9p_i2FIAXh4ADVZC5wzJ-9skKLnIi4LndSDeHggMS_hq0ElSBieFrXGovGyt-U-APz_Gt0Q</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Pribylova, Lenka</creator><creator>Papouskova, Klara</creator><creator>Sychrova, Hana</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiology</title><author>Pribylova, Lenka ; Papouskova, Klara ; Sychrova, Hana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-7e5cd887a4469cb1c30ff5ccfdf1a59ec5cb4e00a52b74ce7a31d4fa6dfc84ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alkali-metal-cation transporters</topic><topic>Amino Acid Sequence</topic><topic>antiporters</topic><topic>cations</topic><topic>Cations - metabolism</topic><topic>cell physiology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>fungus physiology</topic><topic>Gene Expression</topic><topic>genes</topic><topic>Homeostasis</topic><topic>ion transport</topic><topic>microbial genetics</topic><topic>Molecular Sequence Data</topic><topic>mutants</topic><topic>Na+/H+-antiporter</topic><topic>osmotolerance</topic><topic>phenotype</topic><topic>plasma membrane</topic><topic>potassium</topic><topic>Potassium homeostasis</topic><topic>Salt tolerance</topic><topic>Sequence Alignment</topic><topic>Sequence Deletion</topic><topic>sodium</topic><topic>Sodium Chloride - metabolism</topic><topic>Sodium-Hydrogen Exchangers - genetics</topic><topic>Sodium-Hydrogen Exchangers - metabolism</topic><topic>strain differences</topic><topic>ZrNHA1 gene</topic><topic>Zygosaccharomyces</topic><topic>Zygosaccharomyces - genetics</topic><topic>Zygosaccharomyces - physiology</topic><topic>Zygosaccharomyces rouxii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pribylova, Lenka</creatorcontrib><creatorcontrib>Papouskova, Klara</creatorcontrib><creatorcontrib>Sychrova, Hana</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pribylova, Lenka</au><au>Papouskova, Klara</au><au>Sychrova, Hana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiology</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>45</volume><issue>10</issue><spage>1439</spage><epage>1447</epage><pages>1439-1447</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>Antiporters exporting Na+ and K+ in exchange for protons are conserved among yeast species. The only exception so far has been Zygosaccharomyces rouxii, an osmotolerant species closely related to Saccharomyces cerevisiae. Z. rouxii was described as possessing one plasma-membrane antiporter transporting only Na+ (ZrSod2–22p in the CBS 732T type strain). We report the characterization of a second gene, ZrNHA1, encoding a new K+(Na+)/H+-antiporter capable of both K+ and Na+ export. Synteny analyses suggested that ZrSOD2–22 originated by single duplication of the ZrNHA1 gene. Substrate specificities and transport properties of ZrNha1p and ZrSod2–22p were compared upon heterologous expression in S. cerevisiae, and then directly in Z. rouxii. Deletion mutants and phenotype analyses revealed that ZrSod2–22 antiporter is important for Na+ detoxification, probably together with ZrEna1 ATPase; ZrNha1p is indispensable to maintain potassium homeostasis and ZrEna1p is not, in contrast to the situation in S. cerevisiae, involved in this function.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18761413</pmid><doi>10.1016/j.fgb.2008.08.001</doi><tpages>9</tpages></addata></record> |
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| ispartof | Fungal genetics and biology, 2008-10, Vol.45 (10), p.1439-1447 |
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| subjects | Alkali-metal-cation transporters Amino Acid Sequence antiporters cations Cations - metabolism cell physiology Fungal Proteins - genetics Fungal Proteins - metabolism fungus physiology Gene Expression genes Homeostasis ion transport microbial genetics Molecular Sequence Data mutants Na+/H+-antiporter osmotolerance phenotype plasma membrane potassium Potassium homeostasis Salt tolerance Sequence Alignment Sequence Deletion sodium Sodium Chloride - metabolism Sodium-Hydrogen Exchangers - genetics Sodium-Hydrogen Exchangers - metabolism strain differences ZrNHA1 gene Zygosaccharomyces Zygosaccharomyces - genetics Zygosaccharomyces - physiology Zygosaccharomyces rouxii |
| title | The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiology |
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