Characterization and purification of bacteriophages using chromatofocusing

The technique of chromatofocusing was applied to the characterization and purification of three bacteriophages that are routinely used for testing virus filters: φX174, PR772, and PP7. Chemically well-defined eluent buffers were used, instead of the more commonly used chromatofocusing polyampholyte...

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Veröffentlicht in:	Journal of Chromatography A 2008-10, Vol.1207 (1), p.110-121
Hauptverfasser:	Brorson, Kurt, Shen, Hong, Lute, Scott, Pérez, Jessica Soto, Frey, Douglas D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage Bacteriophages - isolation & purification Biological and medical sciences Chromatofocusing Chromatography, Ion Exchange - methods Fundamental and applied biological sciences. Psychology Isoelectric Focusing - methods Isoelectric Point Microbiology pH gradient elution Techniques used in virology Virology Virus characterization Virus purification
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