Specific, rapid, and sensitive enzymatic measurement of sphingomyelin, phosphatidylcholine and lysophosphatidylcholine in serum and lipid extracts

Human serum sphingomyelin (SM) and phosphatidylcholine (PC) play important roles in the development of atherosclerosis. However, there are no rapid and sensitive methods for SM and PC measurement. The present report describes a novel enzymatic method for measuring SM, PC and lysophosphatidylcholine...

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Veröffentlicht in:	Clinical biochemistry 2008-10, Vol.41 (14-15), p.1211-1217
Hauptverfasser:	Hidaka, Hiroya, Yamauchi, Kazuyoshi, Ohta, Hiroyashi, Akamatsu, Taiji, Honda, Takayuki, Katsuyama, Tsutomu
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry - methods Calibration Choline-containing phospholipids Enzymatic measurements Health Humans Hydrolysis Lipids - blood Lipoproteins, HDL - blood Lysophosphatidylcholine Lysophosphatidylcholines - blood Mass Spectrometry Phosphatidylcholine Phosphatidylcholines - blood Phospholipase D - metabolism Phospholipids Reproducibility of Results Sphingomyelin Sphingomyelin Phosphodiesterase - metabolism Sphingomyelins - blood Substrate Specificity Tissue Extracts
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